A5044947111- Receptor Mechanisms and Signaling
- Neuroscience and Neuropharmacology Research
- Neuropeptides and Animal Physiology
- Biochemical Analysis and Sensing Techniques
- Protein Kinase Regulation and GTPase Signaling
- Monoclonal and Polyclonal Antibodies Research
- Protein Tyrosine Phosphatases
- Galectins and Cancer Biology
- Diabetes and associated disorders
- Computational Drug Discovery Methods
- Epigenetics and DNA Methylation
- Microbial Metabolic Engineering and Bioproduction
- Protein Structure and Dynamics
- Neuroinflammation and Neurodegeneration Mechanisms
- Advanced Numerical Methods in Computational Mathematics
- Plant Pathogenic Bacteria Studies
- Glycosylation and Glycoproteins Research
- Adipose Tissue and Metabolism
- Regulation of Appetite and Obesity
- Pancreatic function and diabetes
- Mass Spectrometry Techniques and Applications
- Neurobiology and Insect Physiology Research
- RNA and protein synthesis mechanisms
- Birth, Development, and Health
- CRISPR and Genetic Engineering
Shandong University
2016-2025
First Affiliated Hospital Zhejiang University
2023-2025
Peking University
2013-2025
Qilu Hospital of Shandong University
2023-2025
Center for Life Sciences
2024
Chinese Academy of Sciences
2015-2024
University of Science and Technology of China
2015-2024
The First Affiliated Hospital, Sun Yat-sen University
2023-2024
Sun Yat-sen University
2013-2024
Zhejiang University
2018-2024
Although the innate immune response to induce postischemic inflammation is considered as an essential step in progression of cerebral ischemia injury, role immunity mediator NLRP3 pathogenesis ischemic stroke unknown. In this study, focal was induced by middle artery occlusion −/− , NOX2 or wild-type (WT) mice. By magnetic resonance imaging (MRI), Evans blue permeability, and electron microscopic analyses, we found that deficiency ameliorated injury mice after reducing infarcts blood–brain...
β-Arrestins (βarrs) interact with G protein-coupled receptors (GPCRs) to desensitize protein signaling, initiate signaling on their own, and mediate receptor endocytosis. Prior structural studies have revealed two unique conformations of GPCR-βarr complexes: the "tail" conformation, βarr primarily coupled phosphorylated GPCR C-terminal tail, "core" where, in addition is further engaged transmembrane core. However, relationship these distinct various functions βarrs unknown. Here, we created...
Abstract Specific arrestin conformations are coupled to distinct downstream effectors, which underlie the functions of many G-protein-coupled receptors (GPCRs). Here, using unnatural amino acid incorporation and fluorine-19 nuclear magnetic resonance ( 19 F-NMR) spectroscopy, we demonstrate that receptor phospho-barcodes translated specific β-arrestin-1 direct selective signalling. With its phosphate-binding concave surface, ‘reads’ message in phospho-C-tails phospho-interaction patterns...
Abstract Acute hormone secretion triggered by G protein-coupled receptor (GPCR) activation underlies many fundamental physiological processes. GPCR signalling is negatively regulated β-arrestins, adaptor molecules that also activate different intracellular pathways. Here we reveal TRV120027, a β-arrestin-1-biased agonist of the angiotensin II type 1 (AT1R), stimulates acute catecholamine through coupling with transient potential cation channel subfamily C 3 (TRPC3). We show TRV120027...
The tyrosine phosphorylation barcode encoded in C-terminus of HER2 and its ubiquitination regulate diverse functions. PTPN18 was reported as a phosphatase; however, the exact mechanism by which it defines signaling is not fully understood. Here, we demonstrate that regulates HER2-mediated cellular functions through defining both barcodes. Enzymologic characterization three crystal structures complex with phospho-peptides revealed molecular basis for recognition between specific sites,...
Abstract Direct optimization of the metabolic pathways on chromosome requires tools that can fine tune overexpression a desired gene or optimize combination multiple genes. Although plasmid-dependent has been used for this task, fundamental issues concerning its genetic stability and operational repeatability have not addressed. Here, we describe rapid reliable strategy chromosomal integration gene(s) with copies (CIGMC), which uses flippase from yeast 2-μm plasmid. Using green fluorescence...
Arrestins were initially identified for their role in homologous desensitization and internalization of G protein-coupled receptors. Receptor-bound arrestins also initiate signaling by interacting with other proteins. scaffold MAPK cascades, kinase (MAP3K), (MAP2K), MAPK. In particular, facilitate ERK1/2 activation scaffolding (MAPK), MEK1 Raf (MAPK3). However, the structural mechanism underlying this remains unknown. Here, we investigated arrestin-2 cRaf, MEK1, ERK2 using hydrogen/deuterium...
GPR126 is a member of the adhesion G protein-coupled receptors (aGPCRs) that essential for normal development diverse tissues, and its mutations are implicated in various pathological processes. Here, through screening 34 steroid hormones their derivatives cAMP production, we found progesterone (P4) 17-hydroxyprogesterone (17OHP) could specifically activate trigger downstream Gi signaling by binding to ligand pocket seven-transmembrane domain C-terminal fragment GPR126. A detailed...