A5067022444- Receptor Mechanisms and Signaling
- Mass Spectrometry Techniques and Applications
- Neuropeptides and Animal Physiology
- Protein Structure and Dynamics
- RNA and protein synthesis mechanisms
- Enzyme Structure and Function
- Sepsis Diagnosis and Treatment
- Renin-Angiotensin System Studies
- Monoclonal and Polyclonal Antibodies Research
- Nitric Oxide and Endothelin Effects
- Biochemical and Molecular Research
- Inflammation biomarkers and pathways
- Ion Transport and Channel Regulation
- Inflammatory mediators and NSAID effects
- Green IT and Sustainability
- Neuroscience and Neuropharmacology Research
- Lipid Membrane Structure and Behavior
- Parallel Computing and Optimization Techniques
- Chemokine receptors and signaling
- Amino Acid Enzymes and Metabolism
- Immune Response and Inflammation
- Embedded Systems Design Techniques
- Biochemical Acid Research Studies
- Neurotransmitter Receptor Influence on Behavior
Shandong University
2018-2023
Cangzhou Central Hospital
2022
Peking University
2019
Individual free fatty acids (FAs) play important roles in metabolic homeostasis, many through engagement with more than 40G protein-coupled receptors. Searching for receptors to sense beneficial omega-3 FAs of fish oil enabled the identification GPR120, which is involved a spectrum diseases. Here, we report six cryo-electron microscopy structures GPR120 complex FA hormones or TUG891 and Gi Giq trimers. Aromatic residues inside ligand pocket were responsible recognizing different double-bond...
Compelling evidence has revealed that biased activation of G protein-coupled receptor (GPCR) signaling, including angiotensin II (AngII) type 1 (AT1) plays pivotal roles in vascular homeostasis and injury, but whether a clinically relevant endogenous antagonism AT1 signaling exists under physiological pathophysiological conditions not been clearly elucidated. Here, we show an extracellular matrix protein, cartilage oligomeric protein (COMP), acts as allosteric modulator the its deficiency is...
Abstract Characterization of the dynamic conformational changes in membrane protein signaling complexes by nuclear magnetic resonance (NMR) spectroscopy remains challenging. Here we report site-specific incorporation 4-trimethylsilyl phenylalanine (TMSiPhe) into proteins, through genetic code expansion. Crystallographic analysis revealed structural that reshaped TMSiPhe-specific amino-acyl tRNA synthetase active site to selectively accommodate trimethylsilyl (TMSi) group. The unique up-field...
The adhesion GPCR ADGRG2, also known as GPR64, is a critical regulator of male fertility that maintains ion/pH homeostasis and CFTR coupling. molecular basis ADGRG2 function poorly understood, in part because no endogenous ligands for have been reported, thus limiting the tools available to interrogate activity. It has shown can be activated by peptide, termed p15, derived from its own N-terminal region Stachel sequence. However, low affinity p15 limits utility characterization. In current...
To accomplish concerted physiological reactions, nature has diversified functions of a single hormone at least two primary levels: 1) Different receptors recognize the same hormone, and 2) different cellular effectors couple to hormone–receptor pair [R.P. Xiao, Sci STKE 2001 , re15 (2001); L. Hein, J. D. Altman, B.K. Kobilka, Nature 402 181–184 (1999); Y. Daaka, M. Luttrell, R. Lefkowitz, 390 88–91 (1997)]. Not only these questions lie in heart actions receptor signaling but also dissecting...
Abstracts Protein Phosphatase Mg 2+ /Mn ‐Dependent 1K ( PPM 1K),also named as PP 2Cm or branched‐chain α‐ketoacid dehydrogenase complex phosphatase, is a member of the metal‐dependent phosphatase family and an important metabolic regulator. Single nucleotide polymorphisms SNP s) in contributing to protein functional defects have been found be associated with numerous human diseases, such cardiovascular disease, maple syrup urine type 2 diabetes, neurological disease. N94K identified missense...
Objective. To assess the clinical efficacy of oxymatrine plus antiviral therapy in treatment sepsis and its effects on levels endotoxin inflammatory factors. Methodology. 90 patients with were selected for retrospective analysis assigned to receive either conventional (control group) or (study group). The endpoint was efficacy. Results. There no significant differences baseline patient profile between two groups ( <a:math xmlns:a="http://www.w3.org/1998/Math/MathML" id="M1"> <a:mi>P</a:mi>...
Traditional power management enables CPU to switch among different consumption modes such as running, idle and standby save energy a certain degree. In battery-powered embedded Linux systems, plays an even more important role than in desktops. This paper proposes practical solution dynamic (DPM) based on frequency scaling (DFS). According the system workload, it switches working several predefined settings. presents design implementation of system. Experimental results Intel PXA250-based...
Abstract While one dimensional 1 H nuclear magnetic resonance (1D H-NMR) spectroscopy is of the most important and convenient method for measuring conformation change in biomacromolecules, characterization protein dynamics large membrane complexes by 1D H-NMR remains challenging, due to difficulty spectra assignment, low signal-to-noise ratio (S/N) need amount protein. Here we report site-specific incorporation 4-trimethylsilyl phenylalanine (TMSiPhe) into proteins, through genetic code...