A5037288788- Receptor Mechanisms and Signaling
- Mass Spectrometry Techniques and Applications
- Protein Structure and Dynamics
- Neuropeptides and Animal Physiology
- Enzyme Structure and Function
- Computational Drug Discovery Methods
- RNA and protein synthesis mechanisms
- Click Chemistry and Applications
- Acupuncture Treatment Research Studies
- Viral Infectious Diseases and Gene Expression in Insects
- Lipid Membrane Structure and Behavior
- Nitric Oxide and Endothelin Effects
- Biochemical Acid Research Studies
- Renin-Angiotensin System Studies
- Botulinum Toxin and Related Neurological Disorders
- Melanoma and MAPK Pathways
- Neuroscience and Neuropharmacology Research
- Stroke Rehabilitation and Recovery
- Ion Transport and Channel Regulation
- Amino Acid Enzymes and Metabolism
- Gene Regulatory Network Analysis
- Protein Kinase Regulation and GTPase Signaling
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Monoclonal and Polyclonal Antibodies Research
- Biochemical and Molecular Research
Shandong University
2018-2025
Qilu Hospital of Shandong University
2023
Institute of Biophysics
2019-2021
Chinese Academy of Sciences
2019-2021
Peking University
2019-2021
Individual free fatty acids (FAs) play important roles in metabolic homeostasis, many through engagement with more than 40G protein-coupled receptors. Searching for receptors to sense beneficial omega-3 FAs of fish oil enabled the identification GPR120, which is involved a spectrum diseases. Here, we report six cryo-electron microscopy structures GPR120 complex FA hormones or TUG891 and Gi Giq trimers. Aromatic residues inside ligand pocket were responsible recognizing different double-bond...
Arrestins were initially identified for their role in homologous desensitization and internalization of G protein-coupled receptors. Receptor-bound arrestins also initiate signaling by interacting with other proteins. scaffold MAPK cascades, kinase (MAP3K), (MAP2K), MAPK. In particular, facilitate ERK1/2 activation scaffolding (MAPK), MEK1 Raf (MAPK3). However, the structural mechanism underlying this remains unknown. Here, we investigated arrestin-2 cRaf, MEK1, ERK2 using hydrogen/deuterium...
Abstract Characterization of the dynamic conformational changes in membrane protein signaling complexes by nuclear magnetic resonance (NMR) spectroscopy remains challenging. Here we report site-specific incorporation 4-trimethylsilyl phenylalanine (TMSiPhe) into proteins, through genetic code expansion. Crystallographic analysis revealed structural that reshaped TMSiPhe-specific amino-acyl tRNA synthetase active site to selectively accommodate trimethylsilyl (TMSi) group. The unique up-field...
To accomplish concerted physiological reactions, nature has diversified functions of a single hormone at least two primary levels: 1) Different receptors recognize the same hormone, and 2) different cellular effectors couple to hormone–receptor pair [R.P. Xiao, Sci STKE 2001 , re15 (2001); L. Hein, J. D. Altman, B.K. Kobilka, Nature 402 181–184 (1999); Y. Daaka, M. Luttrell, R. Lefkowitz, 390 88–91 (1997)]. Not only these questions lie in heart actions receptor signaling but also dissecting...
Single-molecule Förster resonance energy transfer (smFRET) is a powerful tool for investigating the dynamic properties of biomacromolecules. However, success protein smFRET relies on precise and efficient labeling two or more fluorophores interest (POI), which has remained highly challenging, particularly large membrane complexes. Here, we demonstrate site-selective incorporation novel unnatural amino acid (2-amino-3-(4-hydroselenophenyl) propanoic acid, SeF) through genetic expansion...
Förster resonance energy transfer (FRET) is a well-established method for studying macromolecular interactions and conformational changes within proteins. Such normally uses fluorescent proteins or chemical-labeling methods which are often only accessible to surface-exposed residues risk-disturbing target protein structures. Here, we demonstrate that the genetic incorporation of synthetic amino acid, L-(7-hydroxycoumarin-4-yl) ethylglycine (Cou) natural endogenous fluorophore Tryptophan...
Abstracts Protein Phosphatase Mg 2+ /Mn ‐Dependent 1K ( PPM 1K),also named as PP 2Cm or branched‐chain α‐ketoacid dehydrogenase complex phosphatase, is a member of the metal‐dependent phosphatase family and an important metabolic regulator. Single nucleotide polymorphisms SNP s) in contributing to protein functional defects have been found be associated with numerous human diseases, such cardiovascular disease, maple syrup urine type 2 diabetes, neurological disease. N94K identified missense...
Abstract While one dimensional 1 H nuclear magnetic resonance (1D H-NMR) spectroscopy is of the most important and convenient method for measuring conformation change in biomacromolecules, characterization protein dynamics large membrane complexes by 1D H-NMR remains challenging, due to difficulty spectra assignment, low signal-to-noise ratio (S/N) need amount protein. Here we report site-specific incorporation 4-trimethylsilyl phenylalanine (TMSiPhe) into proteins, through genetic code...
Correction for ‘Single-molecule FRET and conformational analysis of beta-arrestin-1 through genetic code expansion a Se-click reaction’ by Ming-Jie Han <italic>et al.</italic>, <italic>Chem. Sci.</italic>, 2021, DOI: 10.1039/D1SC02653D.