A5100439612- Advanced Proteomics Techniques and Applications
- Advanced biosensing and bioanalysis techniques
- Nitric Oxide and Endothelin Effects
- Mass Spectrometry Techniques and Applications
- Molecular Sensors and Ion Detection
- Sulfur Compounds in Biology
- Peptidase Inhibition and Analysis
- Luminescence and Fluorescent Materials
- Glycosylation and Glycoproteins Research
- Metabolomics and Mass Spectrometry Studies
- COVID-19 epidemiological studies
- Genomics and Phylogenetic Studies
- Monoclonal and Polyclonal Antibodies Research
- Hand Gesture Recognition Systems
- Analytical Chemistry and Chromatography
- Electrochemical sensors and biosensors
- Analytical chemistry methods development
- Polysaccharides and Plant Cell Walls
- Photosynthetic Processes and Mechanisms
- Muscle activation and electromyography studies
- Gestational Trophoblastic Disease Studies
- Metabolism, Diabetes, and Cancer
- Hydrocarbon exploration and reservoir analysis
- Nerve injury and regeneration
- Seaweed-derived Bioactive Compounds
Shanghai Jiao Tong University
2024-2025
Beijing University of Technology
2025
Sanya University
2025
Henan University
2025
Shandong University of Traditional Chinese Medicine
2025
The Scarborough Hospital
2025
University of Toronto
2025
Fudan University
2014-2024
Zhongshan Hospital
2009-2024
The First Affiliated Hospital, Sun Yat-sen University
2009-2024
Individual free fatty acids (FAs) play important roles in metabolic homeostasis, many through engagement with more than 40G protein-coupled receptors. Searching for receptors to sense beneficial omega-3 FAs of fish oil enabled the identification GPR120, which is involved a spectrum diseases. Here, we report six cryo-electron microscopy structures GPR120 complex FA hormones or TUG891 and Gi Giq trimers. Aromatic residues inside ligand pocket were responsible recognizing different double-bond...
Biological and pharmaceutical samples represent formidable challenges in sample preparation that hold important consequences for bioanalysis genotoxic impurity quantification. This Feature will emphasize significant advances toward the development of rapid, sensitive, selective methods.
Data-independent acquisition (DIA) mass spectrometry-based proteomics generates reproducible proteome data. The complex processing of the DIA data has led to development multiple analysis tools. In this study, we assessed performance five tools (OpenSWATH, EncyclopeDIA, Skyline, DIA-NN, and Spectronaut) using six datasets obtained from TripleTOF, Orbitrap, TimsTOF Pro instruments. By comparing identification quantification metrics examining shared unique cross-tool identifications, evaluated...
The treatment of osteoporotic bone defect remains a big clinical challenge because osteoporosis (OP) is associated with oxidative stress and high levels reactive oxygen species (ROS), condition detrimental for formation. Anti-oxidative nanomaterials such as selenium nanoparticles (SeNPs) have positive effect on osteogenesis owing to their pleiotropic pharmacological activity which can exert anti-oxidative functions prevent loss facilitate regeneration in OP. In the current study strategy...
For the highly efficient extraction of N-glycoproteome, a novel solid-phase method based on oxime click chemistry has been developed. With use newly synthesized aminooxy-functionalized magnetic nanoparticle, oxidized glycan chains glycopeptides readily react with aminooxy groups through chemistry, resulting in selective glycopeptides. Compared to traditional hydrazide chemistry-based method, which takes 12–16 h coupling time, this new renders excellent enrichment performance within 1 h....
Abstract Background Metabolism is increasingly recognized as a key regulator of the function and phenotype primary cellular constituents atherosclerotic vascular wall, including endothelial cells, smooth muscle inflammatory cells. However, comprehensive analysis metabolic changes associated with transition plaque from stable to hemorrhaged lacking. Methods In this study, we integrated two large mRNA expression protein abundance datasets (BIKE, n = 126; MaasHPS, 43) human carotid artery...
Magnetic yolk–shell MSP@ZrO2 microspheres consisting of a movable magnetic supraparticle (MSP) core and crystalline ZrO2 shell were synthesized via two-step controlled "sol–gel" approach for the first time. First, large amount generated hydrolyzate Zr(OH)4 was firmly fixed onto surface cross-linked polymethylacrylic acid matrix strong hydrogen-bonding interaction between carboxyl groups. Then calcination process adopted to convert into continuous simultaneously make crystallized. At same...
Abstract Two polysaccharides were obtained from dandelion roots (DPR) and leaves (DPL) via water extraction ethanol precipitation. Both DPR DPL an acid heteropolysaccharide, with a molecular weight of 5.89 × 10 4 28.2 Da, respectively. Monosaccharide composition results showed that both contained Man, Glc, Rha, GalA, Gal, Ara molar ratios 2.98:1.00:3.67:6.41:2.98:16.70:2.48 1.23:1.00:1.06:5.74:2.88:8.59:1.84, Methylation NMR analysis DPL, triple-helix conformations, mainly composed 4-ɑ-Gal p...
For the first time, mesoporous material has been introduced into <italic>N</italic>-glycoproteome extraction based on a reductive amination reaction, which greatly enhanced enrichment efficiency and deglycosylation efficiency.
The mouse is a valuable model organism for biomedical research. Here, we established comprehensive spectral library and the data-independent acquisition-based quantitative proteome maps 41 organs, including some rarely reported organs such as cornea, retina, nine paired organs. contained 178,304 peptides from 12,320 proteins, 1678 proteins not in previous libraries. Our data suggested that nervous system immune expressed most distinct compared with other We also found characteristic protein...
Abstract Single nanoparticle analysis is crucial for various applications in biology, materials, and energy. However, precisely profiling monitoring weakly scattering nanoparticles remains challenging. Here, it demonstrated that deep learning‐empowered plasmonic microscopy (Deep‐SM) enables precise sizing collision detection of functional chemical biological nanoparticles. Image sequences are recorded by the state‐of‐the‐art during single onto sensor surface. Deep‐SM can enhance signal...
Gliomas, including both glioblastoma multiforme (GBM) and lower-grade glioma (LGG), present a substantial challenge in neuro-oncology because of genetic heterogeneity unsatisfactory prognosis. This study aimed to conduct comprehensive multi-omics analysis gliomas using various bioinformatics approaches identify potential therapeutic targets prognostic markers. A was conducted on 1327 sequencing data samples alongside their relevant clinical information sourced from The Cancer Genome Atlas...
Convolutional neural networks are widely used in gesture recognition research, which employs surface electromyography. However, when processing electromyography data, current deep learning models still face challenges, such as insufficient effective feature extraction, poor performance multi-gesture recognition, and low accuracy recognizing sparse To address these issues, this study proposed a multi-stream adaptive convolutional with residual modules (MSACNN-RM) for integrates multiple...
Although artificial intelligence (AI) has achieved high levels of accuracy in the diagnosis various diseases, its impact on physicians' decision-making performance clinical practice is uncertain. This study aims to assess AI diagnostic physicians with differing self-efficacy under working conditions involving different time pressures.A 2 (independent vs AI-assisted diagnosis) × (no pressure 2-minute limit) randomized controlled experiment multicenter was conducted. Participants diagnosed 10...