Abstract Critical‐sized bone defects, especially for irregular shapes, remain a significant challenge in orthopedics. Although various biomaterials are developed regeneration, their application repair of defects is limited by the complicated preparation procedures involved, and lack shape‐adaptive capacity, physiological adhesion, potent osteogenic bioactivity. In present study, simple strategy precipitation introducing tannic acid (TA) with abundant phenolic hydroxyl groups Fe 3 O 4...

Due to the limited self-repairing capacity after peripheral nerve injuries (PNI), artificial conduits are widely applied facilitate neural regeneration. Exogenous electrical stimulation (ES) that is carried out by conductive conduit regulates biological behavior of Schwann cells (SCs). Meanwhile, a longitudinal surface structure counts guide axonal growth accelerate end-to-end connection. Currently, there no equipped with both conduction and axon-guiding structure. Herein, biodegradable,...

Osteosarcoma (OS), a highly aggressive bone tumor, presents significant challenges in terms of effective treatment. We identified that cellular autophagy was impaired within OS by comparing clinical samples through bioinformatic analyses and further validated the inhibition mitochondrial at transcriptomic level. Based on this finding, we investigated therapeutic potential dual functional metal nanoplatform (MnSx) to facilitate transition from protective effect low-level killing high-level...

The epidemiology and cause of ossification the spinal ligaments (OSL) remains obscure. To date, there is no study that comprehensively evaluates prevalence, distribution, concomitance each type OSL by CT among general Chinese population. We therefore aimed to investigate epidemiological characteristics using whole spine in population examine factors correlate with presence OSL.Ossification posterior longitudinal ligament (OPLL), ligamentum flavum (OLF), anterior (OALL), nuchal (ONL), diffuse...

Injectable materials show their special merits in regeneration of damaged/degenerated bones minimally-invasive approach. calcium phosphate bone cement (CPC) has attracted broad attention for its bioactivity, as compared to non-degradable polymethyl methacrylate cement. However, brittleness, poor anti-washout property and uncontrollable biodegradability are the main challenges limit further clinical application mainly because stone-like dense structure fragile inorganic-salt weakness. Herein,...

Abstract Peripheral nerve injury (PNI) remains a challenging area in regenerative medicine. Nerve guide conduit (NGC) transplantation is common treatment for PNI, but the prognosis of NGC unsatisfactory due to 1) neuromechanical unmatching and 2) intra‐conduit inflammatory microenvironment (IME) resulting from Schwann cell pyroptosis inflammatory‐polarized macrophages. A neuromechanically matched composed regenerated silk fibroin (RSF) loaded with poly(3,4‐ethylenedioxythiophene):...

Abstract Osteoporosis (OP) can result in slower bone regeneration than the normal condition due to abnormal oxidative stress and high levels of reactive oxygen species (ROS), a detrimental for formation, making OP‐related healing significant clinical challenge. As osteogenic differentiation ability marrow mesenchymal stem cells (BMSCs) is closely related regeneration; currently, this study assessed effects Picein on BMSCs vitro osteoporotic defect vivo. Cell viability was determined by CCK‐8...

The treatment of osteoporotic bone defect remains a big clinical challenge because osteoporosis (OP) is associated with oxidative stress and high levels reactive oxygen species (ROS), condition detrimental for formation. Anti-oxidative nanomaterials such as selenium nanoparticles (SeNPs) have positive effect on osteogenesis owing to their pleiotropic pharmacological activity which can exert anti-oxidative functions prevent loss facilitate regeneration in OP. In the current study strategy...

Ferroptosis is a novel form of cell death, which unique modality death and closely associated with iron concentrations, generation reactive oxygen species (ROS), accumulation the lipid species. In present study, anti-ferroptosis effects Engeletin was studied in erastin-induced bone marrow mesenchymal stem cells (BMSCs). After treatment Engeletin, viability determined by CCK-8 assay. The production ROS, malonaldehyde (MDA), Superoxide dismutase (SOD) activities glutathione peroxidase (GSH)...

Oxidative stress is one of the most important factors in changing bone homeostasis. Redox homeostasis plays a key role osteogenic differentiation mesenchymal stem cells (BMSCs) and angiogenesis ability human umbilical vein endothelial (HUVECs) for regeneration. Currently, this study assessed effects punicalagin (PUN) on BMSCs HUVECs. Cell viability was determined by CCK-8 assay. A flow cytometry analysis adopted to detect macrophage polarization. The production reactive oxygen (ROS),...

Osteoporosis (OP) can result in slower bone regeneration than the normal condition due to imbalance between osteogenesis and osteoclastogenesis, making osteoporotic defects healing a significant clinical challenge. Calcium phosphate cement (CPC) is promising substitute material its good osteoinductive activity, however, drawbacks such as fragility, slow degradation rate incapability control loss restrict application treatment. Currently, we developed PLGA electrospun nanofiber sheets carry...

Currently, sonodynamic therapy (SDT) has limited therapeutic outcomes and immune responses, highlighting the urgent need for enhanced strategies that can stimulate robust long-lasting antitumor effects. Microcystis, a notorious microalga, reveals possibility of mediating SDT owing to presence gas vesicles (GVs) phycocyanin (PC). Herein, nontoxic strain Microcystis elabens (labeled Me) is developed as novel agent because it generates O

Abstract Background Peripheral nerve injury (PNI) is commonly observed in clinical practice, yet the underlying mechanisms remain unclear. This study investigated correlation between expression of a Ras-related protein Rab32 and pyroptosis rats following PNI, potential have been explored by which may influence Schwann cells ultimately peripheral regeneration (PNR) through regulation Reactive oxygen species (ROS) levels. Methods The authors induction cell elevated rat model PNI. In vitro...

Low responsiveness to anti-programmed death-1/programmed death-ligand 1 (anti-PD-1/PD-L1) for solid tumors indicates the presence of other immunosuppressive pathways. Siglec15, a newly discovered immune checkpoint, has been reported repress responses in tumor microenvironment (TME) and regulate osteoclast differentiation. However, role Siglec15 treatment bone metastasis remains unclear. Herein, shows significantly higher expression lung adenocarcinoma spinal (LUAD-SM) than para-cancerous...

Abstract Bone repair in patients with osteoporosis is a major challenge. In this study, multi‐enhanced bioactive microsphere scaffolds (abbr. GMA MSs) composed of gelatin framework alendronate (ALN) and magnesium (Mg) spheres encapsulants are developed for repairing osteoporotic bone defects. The MSs prepared via the reverse microemulsion method photocuring process have uniform morphology. Hydrogen (H 2 ) generated from scavenged reactive oxygen species (ROS), regulated macrophage phenotype,...

Objective To investigate the relationship between hypoxia inducible factor-1α (HIF-1α) and autophagic response in osteoarthritic chondrocytes (OA), under inflammatory insult as represented by vitro OA model. Methods Human chondrocyte cell line C28/I2 was cultured both normoxic hypoxic conditions treated with interleukin-1β (IL1β) to emulate vitro. Cellular HIF-1α expression silenced using siRNA transfection cellular (P62/LC3II) damage (COL2A1/MMP13) related proteins were examined western...

Abstract Large segmental bone defects are commonly operated with autologous grafting, which has limited sources and poses additional surgical risks. In this study, we fabricated poly(lactide- co -glycolic acid) (PLGA)/β-tricalcium phosphate (β-TCP) composite membranes by electrostatic spinning further promoted osteogenesis regulating the release of β-TCP in hope replacing grafts clinical practice. The addition improved mechanical strength PLGA 2.55 times. Moreover, could accelerate...

Osteomyelitis with high mortality and disability rates is a common clinical disease caused by bacterial infection that difficult to cure. Considering the stubborn nature depth of tissue infection, rapid effective treatments for osteomyelitis remain an enormous challenge. Calcium hydride (CaH

Abstract Recent research on ferroptosis and cuproptosis has underscored the crucial role of trace element regulation in osteoarthritis (OA) treatment. However, systematically addressing alterations nutrient elements OA cartilage is lacking. This study initiated using clinical specimens to quantify metal concentrations both damaged intact identify deficient within inflammatory senescent microenvironments OA. Based preliminary findings selenium (Se) gallium (Ga) deficiencies cartilage,...

Perioperative pain management in children with Gartland Type III supracondylar humerus fractures (SHF) is crucial but often inadequately addressed, leading to significant experiences. This study aimed evaluate the efficacy and safety of coracobrachialis plane musculocutaneous nerve block (Cora-MNB) compared supraclavicular brachial plexus (SC-BPB) for analgesia pediatric SHF patients. A prospective pilot enrolled 105 patients was performed. Primary outcome postoperative FLACC scale measured...

Background: Non-small cell lung cancer (NSCLC) frequently metastasizes to bone, leading poor prognosis. Siglec15 has been identified as a newly discovered immune checkpoint and exists in variety of tumors. However, the expression function NSCLC bone metastasis remains largely unclear. Methods: correlation between clinicopathological factors patients with were analyzed using The Cancer Genome Atlas (TCGA) dataset. Correlation analysis metastasis-related genes was based on Molecular Signatures...

Recent studies have indicated that graphene and its derivative oxide (GO) engage in a wide range of antibacterial activities with limited toxicity to human cells. Here, we systematically evaluate the dependence GO on size nanoparticles used treatments: compare cytotoxic effects quantum dots (GQDs, <15 nm), small GOs (SGOs, 50–200 large (LGOs, 0.5–3 μ m). We synthesize results bacterial colony count assays SEM-based observations morphological changes assess properties these bring into...