A5100371367- RNA modifications and cancer
- Peroxisome Proliferator-Activated Receptors
- Cancer, Lipids, and Metabolism
- Eicosanoids and Hypertension Pharmacology
- interferon and immune responses
- Immune Response and Inflammation
- Immunotherapy and Immune Responses
- Cancer-related molecular mechanisms research
- Epigenetics and DNA Methylation
- RNA Interference and Gene Delivery
- Ubiquitin and proteasome pathways
- Cancer Immunotherapy and Biomarkers
- Cancer, Hypoxia, and Metabolism
- Circular RNAs in diseases
- Immune Cell Function and Interaction
- Advanced biosensing and bioanalysis techniques
- Peptidase Inhibition and Analysis
- MicroRNA in disease regulation
- Genetic factors in colorectal cancer
- Legume Nitrogen Fixing Symbiosis
- Genomics and Chromatin Dynamics
- Angiogenesis and VEGF in Cancer
- Cancer Genomics and Diagnostics
- Inflammasome and immune disorders
- COVID-19 Impact on Reproduction
University of Chicago
2022-2025
Fujian Medical University
2021-2025
Capital Medical University
2016-2024
Southern Medical University
2024
Nanfang Hospital
2024
Sun Yat-sen University
2019-2024
Xi'an Jiaotong University
2024
George Washington University
2020-2024
The Wistar Institute
2021-2024
University of Iowa
2022-2024
Abstract Diabetic cardiomyopathy (DCM) is characterized by lipid accumulation, mitochondrial dysfunction, and aseptic inflammatory activation. Mitochondria-derived cytosolic DNA has been reported to induce inflammation activating cyclic GMP-AMP synthase (cGAS)/the stimulator of interferon genes (STING) pathway in the adipose, liver, kidney tissues. However, role mtDNA progression DCM unclear. In this study, with an obesity-related mouse model established feeding db/db mice a high-fat diet...
Abstract The Hi-C method is widely used to study the functional roles of three-dimensional (3D) architecture genomes. Here, we integrate Hi-C, whole-genome sequencing (WGS) and RNA-seq 3D genome multiple myeloma (MM) how it associates with genomic variation gene expression. Our results show that interaction matrices are biased by copy number variations (CNVs) can be detect CNVs. Also, combining WGS data improve detection translocations. We find CNV breakpoints significantly overlap...
Abstract As the largest substructures in nucleus, nucleoli are sites of ribosome biogenesis. Increasing evidence indicates that play a key role organization 3D genome architecture, but systematic studies nucleolus-associated chromatin interactions lacking. Here, we developed nucleolus Hi-C (nHi-C) experimental technique to enrich interactions. Using nHi-C experiment, identify 264 high-confidence domains (hNADs) form strong heterochromatin associated with and consist 24% whole HeLa cells....
Abstract Background Cervical cancer is a common malignant tumor in the female. Interleukin (IL)-17A proinflammatory factor and exerts vital function inflammatory diseases cancers. M2 macrophage has been confirmed to promote development. Nevertheless, it not yet known whether IL-17A facilitates cervical development by inducing polarization. Therefore, this study was conducted investigate regulatory effect of on polarization underlying mechanism Methods RT-qPCR utilized for testing expression...
RNA interference (RNAi) has been used widely as a tool for examining gene function and method that allows its use with plant-parasitic nematodes recently described. Here, we modified to analyze the of secreted β-1,4, endoglucanases potato cyst nematode Globodera rostochiensis, first in vivo functional analysis pathogenicity protein nematode. Knockout reduced ability invade roots. We also RNAi show gr-ams-1, main sense organs (the amphids), is essential host location.
Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) binds to death receptors 4 and 5 (DR4, DR5) transduce apoptotic signals. Conatumumab (AMG 655) is an investigational, fully human monoclonal agonist antibody (IgG1) DR5, which induces apoptosis via caspase activation. In this study, we demonstrate that conatumumab activating intracellular caspases in vitro the presence of a cross-linker. We also show has activity vivo inhibits tumor growth colon (Colo205 HCT-15), lung (H2122),...
It has been widely accepted that lymph nodes (LNs) are critical targets of cancer vaccines because antigen presentation and initiation T-cell-mediated immune responses occur primarily at these locations. In this study, amphiphilic diblock copolymer poly(2-ethyl-2-oxazoline)-poly(d,l-lactide) (PEOz-PLA) combined with carboxylterminated-Pluronic F127 was used to construct mixed micelles [carboxylated-nanoparticles (NPs)] for codelivery ovalbumin (OVA) Toll-like receptor-7 agonist CL264...
We develop a method called open chromatin enrichment and network Hi-C (OCEAN-C) for antibody-independent mapping of global interactions. By integrating FAIRE-seq Hi-C, OCEAN-C detects interactions enriched by active cis-regulatory elements. identify more than 10,000 hubs (HOCIs) in human cells, which are mainly promoters enhancers bound many DNA-binding proteins form interaction networks crucial gene transcription. In addition to identifying large-scale topological structures, including...
This study sought to investigate the shift of leading pacemaker locations in healthy and failing mammalian hearts over entire range physiological heart rates (HRs), molecularly characterize spatial regions spontaneous activity.A normal heartbeat originates as an action potential a group cells known sinoatrial node (SAN), located near superior vena cava. HRs anatomical site origin activity adult are dynamically change response various inputs, yet mechanism this is not well understood.Optical...
With increasing attention paid to the concept of microbiota-gut-brain axis, mounting evidence reveals that gut microbiota is involved in a variety neurological and psychiatric diseases. However, changes major depressive disorder (MDD) patients their association with disease mechanisms remain undefined. Fifty MDD sixty healthy controls were recruited from Shanghai Healthy Mental Center, China. Fecal samples collected, compositional characteristics intestinal flora determined by MiSeq...
Trained immunity is characterized by epigenetic and metabolic reprogramming in response to specific stimuli. This rewiring can result increased cytokine effector responses pathogenic challenges, providing nonspecific protection against disease. It may also improve immune established immunotherapeutics vaccines. Despite its promise for next-generation therapeutic design, most current understanding experimentation conducted with complex heterogeneous biologically derived molecules, such as...
The tal-1 proto-oncogene encodes a helix-loop-helix DNA-binding protein that has been implicated in the formation of T cell acute lymphoblastic leukemia (T-ALL). Patients with T-ALL harbor structural rearrangements result from either local DNA deletion or t(1;14)(p34;q11) chromosome translocation. By analyzing chromosomes series patients, we have now identified discrete region wherein most translocation breakpoints occur. Moreover, mapping genomic revealed coding exons are situated on both...
Intrauterine adhesions (IUA) are a significant cause of menstrual disturbance and infertility, but their pathogenesis still remains unclear. Here, we investigated the expression TGF-β CCN2 in IUA endometrial tissue by immunohistochemistry, western blotting qRT-PCR assays, found was significantly increased compared to normal endometrium uterine septum (P<0.01), suggesting that may play an important role formation IUA. Moreover, activity NF-κB signaling pathway also enhanced (P<0.01)...
Schizophrenia is a serious mental illness affecting approximately 20 million individuals globally. Both genetic and environmental factors contribute to the illness. If left undiagnosed untreated, schizophrenia results in impaired social function, repeated hospital admissions, reduced quality of life decreased expectancy. Clinical diagnosis largely relies on subjective evidence, including self-reported experiences, reported behavioural abnormalities followed by psychiatric evaluation. In...
IDN2/RDM12 has been previously identified as a component of the RNA-directed DNA methylation (RdDM) machinery in Arabidopsis thaliana, but how it functions RdDM remains unknown. By affinity purification IDN2, we co-purified two IDN2 paralogs IDP1 and IDP2 (IDN2 PARALOG 1 2). The coiled-coil domain between XS XH domains is essential for homodimerization, whereas C-terminal not required interaction with IDP2. introducing wild-type sequence its mutated derivatives into idn2 mutant...
The chromodomain helicase DNA-binding family of ATP-dependent chromatin remodeling factors play essential roles during eukaryote growth and development. They are recruited by specific transcription regulate the expression developmentally important genes. Here, we describe an unexpected role in non-coding RNA-directed DNA methylation Arabidopsis thaliana. Through forward genetic screens identified PKL, a gene required for developmental regulation plants, as factor promoting transcriptional...
Gastric cancer (GC) has been classified as the fourth leading cause of cancer-related deaths worldwide. Due to their ability suppress expression target genes, microRNAs (miRNAs) are listed one key elements involved in formation and development tumors. This study was therefore conducted investigate effects microRNA-135b (miR-135b) on cisplatin [ds-diamminedichloroplatinum (CDDP)] resistance GC cells through MAPK signaling pathway by targeting mammalian ste20-like kinase 1 (MST1). A...