A5100406500- Receptor Mechanisms and Signaling
- Cardiac Fibrosis and Remodeling
- Total Knee Arthroplasty Outcomes
- Orthopaedic implants and arthroplasty
- HIV Research and Treatment
- Analytical Chemistry and Chromatography
- Pharmacological Effects and Assays
- Orthopedic Infections and Treatments
- Neuropeptides and Animal Physiology
- Cardiovascular Effects of Exercise
- Cardiovascular Function and Risk Factors
- Monoclonal and Polyclonal Antibodies Research
- Immunotherapy and Immune Responses
- Ubiquitin and proteasome pathways
- Protein Kinase Regulation and GTPase Signaling
- 14-3-3 protein interactions
- Protein Interaction Studies and Fluorescence Analysis
- Microtubule and mitosis dynamics
- Mass Spectrometry Techniques and Applications
- Cell Adhesion Molecules Research
- Immune Cell Function and Interaction
- Cardiomyopathy and Myosin Studies
- Nitric Oxide and Endothelin Effects
- Signaling Pathways in Disease
- Glycosylation and Glycoproteins Research
Peking University
2016-2025
Peking University Third Hospital
2016-2025
China University of Geosciences (Beijing)
2024
National Health and Family Planning Commission
2024
Shanghai Institute of Organic Chemistry
2024
Shanghai Jiao Tong University
2024
Chinese Academy of Medical Sciences & Peking Union Medical College
2023-2024
Beijing University of Chinese Medicine
2024
Soochow University
2024
PLA Academy of Military Science
2024
Rapid over-activation of β-adrenergic receptor (β-AR) upon stress leads to cardiac inflammation, a prevailing factor that underlies heart injury. However, mechanisms by which acute β-AR stimulation induce inflammation still remain unknown. Here, we set out identify the crucial role inflammasome/interleukin (IL)-18 in initiating and maintaining inflammatory cascades insult.Male C57BL/6 mice were injected with single dose agonist, isoproterenol (ISO, 5 mg/kg body weight) or saline...
Aims To evaluate the performance of chat generative pretrained transformer (ChatGPT) in key domains clinical pharmacy practice, including prescription review, patient medication education, adverse drug reaction (ADR) recognition, ADR causality assessment and counselling. Methods Questions pharmacist's answers were collected from real cases pharmacist competency assessment. ChatGPT's responses generated by inputting same question into ‘New Chat’ box ChatGPT Mar 23 Version. Five licensed...
Currently, various calculation methods for evaluating blood-loss in patients with total knee arthroplasty (TKA) are applied clinical practice. However, different may yield results. The purpose of this study was to determine the most reliable method calculating after primary TKA. We compared 245 who underwent unilateral TKA from February 2010 August 2011. calculated using four methods: Gross equation, hemoglobin (Hb) balance, Orthopedic Surgery Transfusion Hemoglobin European Overview...
Abstract Membrane nanotubes (MNTs) act as “highways” between cells to facilitate the transfer of multiple signals and play an important role in many diseases. Our previous work reported on mitochondria via MNTs cardiomyocytes (CMs) cardiac myofibroblasts (MFs); however, elucidation underlying mechanism pathophysiological significance this requires additional study. In study, we determined that mean movement velocity CMs MFs was approximately 17.5 ± 2.1 nm/s. Meanwhile, treatment with...
Heart failure (HF), which is the terminal stage of many cardiovascular diseases, associated with low survival rates and a severe financial burden. The mechanisms, especially molecular mechanism combined new theories, underlying pathogenesis HF remain elusive. We demonstrate that phosphorylation-regulated dynamic liquid-liquid phase separation HIP-55 (hematopoietic progenitor kinase 1-interacting protein 55 kDa) protects against HF. Fluorescence recovery after photobleaching assay,...
The action of β-adrenergic receptors (β-ARs) induces cardiac fibroblast (CF) proliferation and collagen synthesis is a major source the fibrosis caused by various diseases. Recently, microRNA-214 (miR-214) was found to play an important role in pathogenesis remodelling. In present study, we examined underlying mechanism miR-214 isoproterenol (ISO, β-AR agonist)-induced CF synthesis. expression increased both ISO-mediated fibrotic heart tissue fibroblasts. Downregulation antagonists...
Regular exercise can protect the heart against external stimuli, but mechanism is not well understood. We determined role of adenosine monophosphate-activated protein kinase (AMPK) in regulating swimming exercise-mediated cardiac protection β-adrenergic receptor overstimulation with isoproterenol (ISO) mice. Ten-week-old AMPKα2+/+ and AMPKα2-knockout (AMPKα2-/-) littermates were subjected to 4 weeks training (50 min daily, 6 days a week) or housed under sedentary conditions. The mice...
Abstract Ischemic heart disease is a leading cause of death worldwide. Myocardial infarction (MI) results in cardiac damage due to cell and insufficient cardiomyocyte self-renewal. Ferroptosis, novel type death, has recently been shown as key after MI. However, the complicated regulation mechanisms involved ferroptosis, especially how ferroptosis integrated into classical survival/death pathways, are still unclear. Here, we discovered that HIP-55, adaptor protein, acts hub protein for...
Rheumatoid arthritis (RA) is a chronic inflammatory disease characterized by synovial fibroblast hyperplasia and bone erosion. Fibroblast-like synoviocytes (FLS) play pivotal role in RA pathogenesis through aggressive migration matrix invasion, certain proinflammatory cytokines may affect synoviocyte invasion. Whether interleukin (IL)-21 influences this process remains controversial. Here, we evaluated the potential regulatory effect of IL-21 on migration, invasion metalloproteinase (MMP)...
Danshensu, the effective ingredient of plant Salvia miltiorrhiza (Danshen), has been widely used for treatment cardiovascular diseases. Cardiac fibrosis is an important process in pathological cardiac remodeling and leads to heart failure. We investigated effect Danshensu on β-adrenergic receptor (β-AR)-mediated involved signaling transduction. inhibited cardiofibroblast proliferation collagen I synthesis induced by isoproterenol (ISO), a selective β-AR agonist. Phosphorylation p38...
Cardiac fibrosis is a common feature of advanced coronary heart disease and characteristic disease. However, currently available drugs against cardiac are still very limited. Here, we have assessed the role isopropyl 3-(3,4-dihydroxyphenyl)-2-hydroxylpropanoate (IDHP), new metabolite Danshen Dripping Pills, in mediated by β-adrenoceptor agonist, isoprenaline, its underlying mechanisms.Identification IDHP was identified mass spectrometry, proton carbon nuclear magnetic resonance spectra....
Structures of prostaglandin receptor EP2 revealed its agonists’ selectivity and G protein coupling mechanism.
Cardiac aging constitutes a significant risk factor for cardiovascular diseases prevalent among the elderly population. Urgent attention is required to prioritize preventive and management strategies age-related conditions safeguard well-being of individuals. In response this critical challenge, Aging Biomarker Consortium (ABC) China has formulated an expert consensus on cardiac biomarkers. This draws upon latest scientific literature clinical expertise provide comprehensive assessment...
A growing body of evidence has demonstrated that p38 mitogen-activated protein kinase (MAPK) a crucial role in various physiological and pathological processes mediated by β<sub>2</sub>-adrenergic receptors (β<sub>2</sub>-ARs). However, the detailed mechanism β<sub>2</sub>-ARs-induced MAPK activation not yet been fully defined. The present study demonstrates novel kinetic model induced β<sub>2</sub>-ARs human embryonic kidney 293A cells. β<sub>2</sub>-AR agonist isoproterenol time-dependent...
BACKGROUND AND PURPOSE IL-6 plays crucial roles in cardiac hypertrophy, fibrosis and heart failure. Activation of β-adrenoceptors induced production neonatal mouse fibroblasts (NMCFs) through a G(s) /adenylate cyclase/cAMP/p38 MAPK pathway but independent PKA. However, how cAMP activates p38 is still not defined. In this study, we have assessed the role exchange protein directly activated by (Epac) PKCδ activation stimulated β-adrenoceptor agonist isoprenaline NMCFs. EXPERIMENTAL APPROACH...
Early identification for heart failure (HF) may be useful disease modifying treatment in order to reduce progression or even reverse it. In our previous studies, we have revealed a group of heat shock proteins (HSPs) which might related neonatal rat cardiomyocyte hypertrophy by proteomic approach. Here, confirm that HSPs, including HSP27 and HSP70, altered the early stage cardiac remodeling vivo animal model. Furthermore, plasma concentrations those HSPs their potential screening value were...