A5100336051- Congenital heart defects research
- Tissue Engineering and Regenerative Medicine
- Cardiac Fibrosis and Remodeling
- RNA modifications and cancer
- Single-cell and spatial transcriptomics
- Cardiac Ischemia and Reperfusion
- Hippo pathway signaling and YAP/TAZ
- Mitochondrial Function and Pathology
- Immune cells in cancer
- RNA Research and Splicing
- Genomics and Chromatin Dynamics
- MicroRNA in disease regulation
- Cancer Immunotherapy and Biomarkers
- Electrospun Nanofibers in Biomedical Applications
- Bladder and Urothelial Cancer Treatments
- Cardiomyopathy and Myosin Studies
- Chromatin Remodeling and Cancer
- Pluripotent Stem Cells Research
- RNA and protein synthesis mechanisms
- Cell death mechanisms and regulation
- Non-Invasive Vital Sign Monitoring
- Neuroscience and Neural Engineering
- Gene Regulatory Network Analysis
- Hemodynamic Monitoring and Therapy
- Cancer-related molecular mechanisms research
Chinese Academy of Medical Sciences & Peking Union Medical College
2016-2025
Cangzhou Central Hospital
2025
Gansu Provincial Hospital
2024
Icahn School of Medicine at Mount Sinai
2024
Academy of Medical Sciences
2023
Fu Wai Hospital
2023
State Key Laboratory of Cardiovascular Disease
2023
Academia Sinica
2023
Beihang University
2023
Capital Medical University
2022
Enhancer RNA (eRNA) is a type of noncoding transcribed from the enhancer. Although critical roles eRNA in gene transcription control have been increasingly realized, systemic landscape and potential function eRNAs cancer remains largely unexplored. Here, we report integration multi-omics pharmacogenomics data across large-scale patient samples cell lines. We observe cancer-/lineage-specificity eRNAs, which may be driven by tissue-specific TFs. are involved multiple signaling pathways through...
Human cancer cell lines are fundamental models for research and therapeutic strategy development. However, there is no characterization of circular RNAs (circRNAs) in a large number lines.Here, we apply four circRNA identification algorithms to heuristically characterize the expression landscape circRNAs across ~ 1000 human from CCLE polyA-enriched RNA-seq data. By using integrative analysis experimental approaches, explore landscape, biogenesis, functional consequences, drug response...
In metastatic urothelial cancer (mUC), cisplatin versus carboplatin leads to durable disease control in a subset of patients. The IMvigor130 trial reveals more favorable effects with atezolizumab combined gemcitabine and (GemCis) (GemCarbo). This study investigates the immunomodulatory as potential explanation for these observations. Our findings indicate that improved outcomes GemCis GemCarbo are primarily observed patients pretreatment tumors exhibiting features restrained adaptive...
A comprehensive understanding of the evolution immune landscape in humans across entire lifespan at single-cell transcriptional and protein levels, during development, maturation senescence is currently lacking. We recruited a total 220 healthy volunteers from Shanghai Pudong Cohort (NCT05206643), spanning 13 age groups 0 to over 90 years, profiled their peripheral cells through RNA-sequencing coupled with single T cell B receptor sequencing, high-throughput mass cytometry, bulk flow...
Lactate produced during ischemia-reperfusion injury is known to promote lactylation of proteins, which play controversial roles. By analyzing the lactylomes and proteomes mouse myocardium using mass spectrometry, we show that both Serpina3k protein expression its at lysine 351 are increased upon reperfusion. Both human homolog, SERPINA3, abundantly expressed in cardiac fibroblasts, but not cardiomyocytes. Biochemically, enhances stability. Using knockout mice overexpressing...
Abstract Cardiac maturation lays the foundation for postnatal heart development and disease, yet little is known about contributions of microenvironment to cardiomyocyte maturation. By integrating single-cell RNA-sequencing data mouse hearts at multiple stages, we construct cellular interactomes regulatory signaling networks. Here report switching fibroblast subtypes from a neonatal adult state this drives Molecular functional cardiomyocytes human embryonic stem cell-derived are considerably...
Superenhancers (SEs) are large genomic regions with a high density of enhancer marks. In cancer, SEs found near oncogenes and dictate cancer gene expression. However, how oncogenic regulated remains poorly understood. Here, we show that INO80, chromatin remodeling complex, is required for SE-mediated transcription tumor growth in melanoma. The expression Ino80, the SWI/SNF ATPase, elevated melanoma cells patient melanomas compared normal melanocytes benign nevi. Furthermore, Ino80 silencing...
Cell sheet engineering has been proven to be a promising strategy for cardiac remodeling post-myocardial infarction. However, insufficient mechanical strength and low cell retention lead limited therapeutic efficiency. The thickness area of artificial patches also affect their Cardiac prepared by combining sheets with electrospun nanofibers, which can transplanted sutured the surface infarcted heart, promise solve this problem. Here, we fabricated novel patch stacking brown adipose-derived...
Reperfusion therapy is critical to myocardial salvage in the event of a infarction but complicated by ischemia-reperfusion injury (IRI). Limited understanding spatial organization cardiac cells, which governs cellular interaction and function, has hindered search for targeted interventions minimizing deleterious effects IRI.We used imaging mass cytometry characterize distribution dynamics cell phenotypes communities mouse left ventricle following IRI. Heart sections were collected from 12...
Thoracic aortic aneurysms (TAAs) develop asymptomatically and are characterized by dilatation of the aorta. This is considered a life-threating vascular disease due to risk rupture without effective treatments. The current understanding pathogenesis TAA still limited, especially for sporadic TAAs known genetic mutation. Sirtuin 6 (SIRT6) expression was significantly decreased in tunica media human tissues. Genetic knockout Sirt6 mouse smooth muscle cells accelerated formation rupture,...
Rationale: While cell-cell interaction plays a critical role in physiology and disease, comprehensive understanding of its dynamics vascular homeostasis diseases is yet absent. Methods: Here, by use single-cell RNA-sequencing multi-color staining, we delineate the cellular composition spatial characterization human aorta with or without aortic dissection (AD). Results: Scrutinization cell subtype alterations revealed significantly changed fibroblast (FB)-smooth muscle (SMC) interactions AD....
Immune checkpoint blockade (ICB) therapies exhibit substantial clinical benefit in different cancers, but relatively low response rates the majority of patients highlight need to understand mutual relationships among immune features. Here, we reveal overall positive correlations checkpoints and cell populations. Clinically, benefiting from ICB exhibited increases for both stimulatory inhibitory features after initiation therapy, suggesting that activation microenvironment might serve as...
Abstract Cardiovascular diseases are the most common cause of death globally. Accurately modeling cardiac homeostasis, dysfunction, and drug response lies at heart research. Adult human primary cardiomyocytes (hPCMs) a promising cellular model, but unstable isolation efficiency quality, rapid cell in culture, unknown to cryopreservation prevent them from becoming reliable flexible vitro model. Combing use reversible inhibitor myosin II ATPase, (-)-blebbistatin (Bleb), multiple optimization...
Fulminant myocarditis (FM) is a life-threatening inflammatory disease. However, the mechanisms underlying its acute onset are unknown. By dynamic cardiac function measurement, we discovered that initiation of sudden hemodynamic collapse was on day 4 in mouse model FM. Single-cell RNA-sequencing study revealed healthy cardiomyocytes (CMs) lost their contractile and metabolic differentiated into pro-angiogenic pro-inflammatory CMs. Meanwhile, neutrophils, most expanded immune cells, exhibited...
ABSTRACT Background: Atrial fibrillation (AF) patients undergoing radiofrequency ablation require diligent postoperative care for optimal outcomes. This study investigated the impact of combining mind mapping with behavior rating scales on medical compliance in this population. Methods: A total 108 AF post‐radiofrequency were randomly assigned to a control group ( n = 54) or an intervention 54). Both groups received standard education, while additionally education using and scales....
Cardiac differentiation from human pluripotent stem cells provides a unique opportunity to study heart development in vitro and offers potential cell source for cardiac regeneration. Compared the large body of studies investigating maturation cardiomyocyte subtype-specific induction, molecular events underlying lineage commitment at early stage remain poorly characterized.In order uncover key regulators controlling state during differentiation, we performed single-cell RNA-Seq sequencing...
Rationale: Timely inhibition of inflammation and initiation resolution are important to repair injured tissues. MST1/2 (mammalian STE20-like protein kinase 1/2) acts as a regulator macrophage-associated immune responses bacterial infections. However, the role in regulating macrophage phenotype function myocardial infarction (MI) remains unclear. Objective: To determine underlying mechanism cardiac post-MI. Methods Results: Using LysMCre -mediated Mst1/2 -deficient mice, we found that MST1...
Serine/threonine-protein kinases 3 and 4 (STK3 STK4, respectively) are key components of the Hippo signaling pathway, which regulates cell proliferation death provides a potential therapeutic target for acute myeloid leukemia (AML). Herein, we report structure-based design series pyrrolopyrimidine derivatives as STK3 STK4 inhibitors. In an initial screen, compounds exhibited low nanomolar potency against both STK4. Crystallization compound 6 with revealed two-point hinge binding in...
Cardiac aging constitutes a significant risk factor for cardiovascular diseases prevalent among the elderly population. Urgent attention is required to prioritize preventive and management strategies age-related conditions safeguard well-being of individuals. In response this critical challenge, Aging Biomarker Consortium (ABC) China has formulated an expert consensus on cardiac biomarkers. This draws upon latest scientific literature clinical expertise provide comprehensive assessment...