A5091188597- Peroxisome Proliferator-Activated Receptors
- Eicosanoids and Hypertension Pharmacology
- Cholesterol and Lipid Metabolism
- Adipokines, Inflammation, and Metabolic Diseases
- Neurological Disorders and Treatments
- Cell Adhesion Molecules Research
- Adipose Tissue and Metabolism
- Metabolism, Diabetes, and Cancer
- Nitric Oxide and Endothelin Effects
- Kruppel-like factors research
- Inflammasome and immune disorders
- Drug Transport and Resistance Mechanisms
- Natural Antidiabetic Agents Studies
- interferon and immune responses
- Cancer-related gene regulation
- Pulmonary Hypertension Research and Treatments
- Cancer, Lipids, and Metabolism
- Inflammatory mediators and NSAID effects
- MicroRNA in disease regulation
- Lipid metabolism and biosynthesis
- Cancer-related molecular mechanisms research
- Hormonal Regulation and Hypertension
- Angiogenesis and VEGF in Cancer
- Virus-based gene therapy research
- Electrospun Nanofibers in Biomedical Applications
East China Normal University
2022-2025
Affiliated Hospital of North Sichuan Medical College
2024
Dalian Medical University
2013-2024
Anhui Normal University
2024
North Sichuan Medical University
2024
Chongqing Medical University
2024
Second Affiliated Hospital of Chongqing Medical University
2024
Peking University
2012-2021
China Fishery Machinery and Instrument Research Institute
2012-2020
Rockefeller University
2020
Endothelial cells (ECs) respond to changes in mechanical forces, leading the modulation of signaling networks and cell function; an example is inhibition EC proliferation by steady laminar flow. MicroRNAs (miRs) are short noncoding 20–22 nucleotide RNAs that negatively regulate expression target genes at posttranscriptional level. This study demonstrates miRs involved flow regulation gene ECs. With use microRNA chip array, we found shear stress (12 dyn/cm 2 , 12 h) regulated many miRs,...
Peroxisome proliferator-activated receptor-γ (PPAR-γ) is a ligand-activated nuclear receptor that has an essential role in adipogenesis and glucose homeostasis. PPAR-γ expressed vascular tissues including endothelial cells (ECs). activity can be regulated by many pathophysiological pharmacological agonists. However, the of activation ECs remains unclear. In this study, we examined effect constitutive on phenotypic modulation ECs. Adenovirus-mediated expression constitutively active mutant...
The migration of vascular smooth muscle cells (VSMCs) plays an essential role during the development atherosclerosis and restenosis. Extensive studies have implicated importance extracellular matrix (ECM)-degrading proteinases in VSMC migration. A recently described family proteinases, a disintegrin metalloproteinase with thrombospondin motifs (ADAMTs), is capable degrading ECM proteins. Here, we sought to determine whether ADAMTS-7 involved neointima formation response injury. protein...
5' Adenosine monophosphate-activated protein kinase (AMPK) interacts with peroxisome proliferator-activated receptor δ (PPARδ) to induce gene expression synergistically, whereas the activation of AMPK inhibits endoplasmic reticulum (ER) stress. Whether vascular benefits antidiabetic drug metformin (AMPK activator) in diabetes mellitus and obesity is mediated by PPARδ remains unknown. We aim investigate whether crucial for ameliorating ER stress endothelial dysfunction induced high-fat...
MicroRNAs (miRs) can regulate many cellular functions, but their roles in regulating responses of vascular endothelial cells (ECs) to mechanical stimuli remain unexplored. We hypothesize that the physiological ECs are regulated by not only mRNA and protein signaling networks, also expression corresponding miRs. EC growth arrest induced pulsatile shear (PS) flow is an important feature for regulation ECs. miR profiling showed 21 miRs differentially expressed (8 up- 13 downregulated) response...
Objective— Endothelial activation is implicated in atherogenesis and diabetes. The role of peroxisome proliferator-activated receptor-δ (PPAR-δ) endothelial remains poorly understood. In this study, we investigated the anti-inflammatory effect PPAR-δ mechanism involved. Methods Results— human umbilical vein cells (HUVECs), synthetic ligands GW0742 GW501516 significantly inhibited tumor necrosis factor (TNF)-α–induced expression vascular cell adhesion molecule-1 E-selectin (assayed by...
Epoxyeicosatrienoic acids (EETs), as metabolites of arachidonic acid, may function antihypertensive and antiatherosclerotic mediators for vasculature. EETs are degraded by soluble epoxide hydrolase (sEH). Pharmacological inhibition genetic ablation sEH have been shown to increase the level EETs, treating angiotensin II (Ang II)-infused hypertension rats with sEH-selective inhibitors increased levels attendant decrease in systolic blood pressure. To elucidate mechanisms which Ang regulates...
Background and Purpose Nuciferine, an alkaloid found in Nelumbo nucifera leaves, alleviates dyslipidemia vivo . However, whether it improves liver injury diabetic conditions the underlying mechanism is unclear. The present study aimed to investigate effects of nuciferine on lipid glucose metabolism a murine model Type 2 diabetes mellitus (T2DM) determine mechanisms these effects. Experimental Approach A T2DM was induced by high‐fat diet (HFD) feeding combined with streptozocin (STZ)...
Primary nonalcoholic fatty liver disease is one of the most common forms chronic diseases and associated with insulin-resistant states such as diabetes obesity. Recent work has revealed potential implications peroxisome proliferator-activated receptor-δ (PPARδ) in lipid homeostasis insulin resistance. In this study, we examined effect PPARδ on sterol regulatory element-binding protein-1 (SREBP-1), a pivotal transcription factor controlling lipogenesis hepatocytes. Treatment GW0742, agonist,...
The peroxisome proliferator-activated receptors (PPARs) are ligand-activated transcription factors of the nuclear receptor superfamily. Upon ligand binding, PPARs activate target gene and regulate a variety important physiological processes such as lipid metabolism, inflammation, wound healing. Here, we describe first database PPAR genes, PPARgene. Among 225 experimentally verified 83 for PPARα, PPARβ/δ, 104 PPARγ. Detailed information including tissue types, species, reference PubMed IDs...
Prostaglandin E2 (PGE2) plays an important role in vascular homeostasis. Its receptor, E-prostanoid receptor 4 (EP4) is essential for physiological remodeling of the ductus arteriosus (DA). However, EP4 pathological remains largely unknown. We found that chronic angiotensin II (AngII) infusion mice with smooth muscle cell (VSMC)-specific gene knockout (VSMC-EP4-/-) frequently developed aortic dissection (AD) severe elastic fiber degradation and VSMC dedifferentiation. AngII-infused...
Using non-selective and selective protein kinase C (PKC) epsilon delta isoform inhibitors, we tested the hypothesis that cardioprotective phenotype invoked by postconditioning (postcon) is dependent on PKC signalling. Furthermore, determined whether alters pPKCepsilon and/or pPKCdelta in cytosolic mitochondrial fractions.Male Sprague-Dawley rats underwent 30 min left coronary artery (LCA) occlusion followed 3 h of reperfusion. Rats were randomised to following groups: Untreated, no...
Vascular endothelial cells (ECs) confer atheroprotection at locations of the arterial tree where pulsatile laminar flow (PS) exists with a high shear stress and large net forward direction. We investigated whether PS-induced expression transcription factor Krüppel-Like Factor 2 (KLF2) in cultured ECs its mouse aorta is regulated by AMP-activated protein kinase (AMPK).AMPK inhibition Compound C or siRNA had significant blocking effect on KLF2 expression. The induction PS led to increase eNOS...
Homocysteine (Hcy) is epidemiologically related to insulin resistance, which has been speculated be a low-grade systemic inflammatory condition. Resistin acts as critical mediator of resistance associated with conditions. We aimed determine whether Hcy can induce by directly regulating the expression and secretion resistin from adipose tissue.The effect on was investigated using primary rat adipocytes mice hyperhomocysteinemia (HHcy).Hcy impaired glucose transport and, particularly,...
Rationale : Phenotypic modulation of smooth muscle cells (SMCs), which are located in close proximity to endothelial (ECs), is critical regulating vascular function. The role flow-induced shear stress the SMC phenotype has not been well defined. Objective objective was elucidate on ECs modulating and its underlying mechanism. Methods Results Application (12 dyn/cm 2 ) cocultured with SMCs modulated from synthetic contractile state, upregulation markers, downregulation proinflammatory genes,...
Ghrelin, a gastric hormone, provides hunger signal to the central nervous system stimulate food intake. Mammalian target of rapamycin (mTOR) is an intracellular fuel sensor critical for cellular energy homeostasis. Here we showed reciprocal relationship mTOR signaling and ghrelin during changes in status. activity was down-regulated, whereas preproghrelin circulating were increased by fasting. In db/db mice, enhanced, decreased. Inhibition stimulated expression mRNA plasma both wild-type...