A5062952612- Immunotherapy and Immune Responses
- Peroxisome Proliferator-Activated Receptors
- Inflammasome and immune disorders
- Cervical Cancer and HPV Research
- Monoclonal and Polyclonal Antibodies Research
- Pancreatic function and diabetes
- Hippo pathway signaling and YAP/TAZ
- Virus-based gene therapy research
- Liver Disease Diagnosis and Treatment
- Glycosylation and Glycoproteins Research
- Immune cells in cancer
- Escherichia coli research studies
- Angiogenesis and VEGF in Cancer
- Galectins and Cancer Biology
- Bacteriophages and microbial interactions
- Biomarkers in Disease Mechanisms
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Cardiac Fibrosis and Remodeling
- Blood groups and transfusion
- Chromatography in Natural Products
- Viral Infectious Diseases and Gene Expression in Insects
- Eicosanoids and Hypertension Pharmacology
- Liver physiology and pathology
- Lipid metabolism and biosynthesis
- Metabolism, Diabetes, and Cancer
Xi'an Jiaotong University
2016-2025
Baogang Group (China)
2018
Dalian Medical University
2017
Cleveland Clinic
2017
Shantou University
2007
Shantou University Medical College
2007
Background and Purpose Nuciferine, an alkaloid found in Nelumbo nucifera leaves, alleviates dyslipidemia vivo . However, whether it improves liver injury diabetic conditions the underlying mechanism is unclear. The present study aimed to investigate effects of nuciferine on lipid glucose metabolism a murine model Type 2 diabetes mellitus (T2DM) determine mechanisms these effects. Experimental Approach A T2DM was induced by high‐fat diet (HFD) feeding combined with streptozocin (STZ)...
Peroxisome proliferator–activated receptor γ (PPARγ) is a member of the nuclear superfamily and polarizes macrophages into an anti-inflammatory M2 state. Integrins are transmembrane receptors that drive various cellular functions, including monocyte adhesion foam cell formation. In this study, we first reported expression integrins αV β5 was up-regulated by PPARγ activation in RAW264.7 cells human peripheral blood monocytes. Luciferase reporter ChIP assay revealed directly bound to potential...
Excessive activation of the NLRP3 inflammasome is implicated in cardiovascular diseases. Statins exert an anti-inflammatory effect independent their cholesterol-lowering effect. This study investigated potential role statins endothelial cells (ECs). Western blotting and quantitative reverse-transcription polymerase chain reaction showed that oxidized low-density lipoprotein (ox-LDL) or tumor necrosis factor α (TNF<i>α</i>) activated ECs. Simvastatin mevastatin significantly suppressed...
Procyanidins, a subclass of flavonoids found in commonly consumed foods, possess potential anti-inflammatory activity. Manipulation M1/M2 macrophage homeostasis is an effective strategy for the treatment metabolic inflammatory diseases. The objective this study was to determine effect procyanidins on polarization. Procyanidin B2 (PCB2), most widely distributed natural procyanidins, enhanced expressions M2 markers (Arg1, Ym1 and Fizz1). PCB2 activated peroxisome proliferator-activated...
Background & AimsNOTCH signaling in liver sinusoidal endothelial cells (LSECs) regulates fibrosis, a pathological feature of chronic diseases. POFUT1 is an essential regulator NOTCH signaling. Here, we investigated the role LSECs-expressed fibrosis.MethodsEndothelial-specific Pofut1 knockout mice were generated and subjected to experimental fibrosis by carbon tetrachloride exposure or common bile duct ligation. Liver samples assessed ELISA, histology, electron microscopy, immunostaining RNA...
Background and Purpose Nuciferine, a constituent of lotus leaf, is an aromatic ring‐containing alkaloid, with antioxidative properties. We hypothesize nuciferine might affect vascular reactivity. This study aimed at determining the effects on vasomotor tone underlying mechanism Experimental Approach Nuciferine‐induced relaxations in rings rat main mesenteric arteries were measured by wire myographs. Endothelial NOS ( eNOS ) was determined immunoblotting. Intracellular NO production HUVECs Ca...
The endothelial-to-mesenchymal transition (EndoMT) is a cellular process featuring decreased expression of endothelial marker genes but increased mesenchymal genes. EndoMT involved in dysfunction and the pathogenesis atherosclerosis. To investigate dynamic vascular cells under atheroprotective pulsatile shear stress (PS) atheroprone oscillatory (OS), we analyzed RNA sequencing data from multitimepoint shear-stress experiments. This unbiased analysis involving next-generation confirmed that...
Background Cardiac fibrosis is a core pathological process associated with heart failure. The recruitment and differentiation of primitive fibroblast precursor cells bone marrow origin play critical role in interstitial cardiac fibrosis. KCa3.1 channels are expressed both ventricular fibroblasts circulating mononuclear rats upregulated by angiotensin II . We hypothesized that mediate the inflammatory microenvironment heart, promoting infiltrated marrow-derived to differentiate into...
Hypertension is a high-risk factor for developing coronary heart disease and stroke. Endothelial dysfunction arterial remodeling can lead to increased vascular wall thickness stiffness. Previous studies showed that microRNA-483 (miR-483) enhances endothelial cell (EC) function. Here, we investigated the protective role of miR-483 in hypertension. Data collected from two patient cohorts serum miR-483-3p level was associated with progression hypertension positively correlated In cultured ECs,...
Abstract Mammalian sexual reproduction critically relies on the generation of haploid gametes following a specialized cell division process known as meiosis. Here, we demonstrate that N-6 Adenine-Specific DNA methyltransferase 1 (N6AMT1) plays crucial role in progression meiosis during spermatogenesis, follows. N6AMT1 was expressed germ cells throughout entire with peak mRNA levels spermatocytes at prophase I stage Germ cell-specific deletion N6amt1 mice resulted male subfertility well...
Hyperhomocysteinemia (HHcy) is an independent risk factor for cardiovascular diseases (CVDs). Stachydrine (STA) active component in Chinese motherwort Leonurus heterophyllus sweet, which has been widely used gynecological and disorders. This study aimed to examine the effects of STA on homocysteine (Hcy)-induced endothelial dysfunction.The vascular relaxation rat thoracic aortas (TA), mesenteric arteries (MA) renal (RA) were measured by using Multi Myograph System. The levels nitric oxide...
Heart failure is associated with sympatho-βAR (β-adrenoceptor) activation and cardiac fibrosis. Gal-3 (galectin-3) KCa3.1 channels that are upregulated in diverse cells of diseased heart implicated mediating myocardial inflammation It remains unclear whether interacts leading to fibrosis the setting βAR activation. We tested effect blocker TRAM-34 on cardiac-restricted β2-TG (β2AR overexpressed transgenic) mice determined expression β2-TG×Gal-3-/- mouse hearts. Mechanisms induced fibroblast...
Procyanidin B2 (PCB2), a natural flavonoid, has been demonstrated to exert anti-oxidation and anti-inflammatory effects on hepatic diseases. Increasing evidence shows the hepatoxicity of nicotine. However, whether PCB2 protects against nicotine-induced underlying mechanisms remains uncharacterized. Here, we reported that nicotine promoted hepatocyte pyroptosis, as evidenced by elevation propidium iodide (PI)-positive cells, activation Caspase-1 gasdermin D (GSDMD), enhanced expression...
Hyperlipidemia with high blood levels of free fatty acids (FFA) is the leading cause non-alcoholic steatohepatitis. CCN1 a secreted matricellular protein that drives various cellular functions, including proliferation, migration, and differentiation. However, its role in mediating FFA-induced pro-inflammatory cell death underlying molecular mechanisms have not been characterized. In this study, we demonstrated was upregulated livers obese mice. The increase evaluated vitro by treating...
Protein tyrosine phosphatase, non-receptor type 11 (PTPN11) is a multifunctional phosphatase and has significant part in many types of tumors. As yet, neither the expression profile PTPN11 nor its significance pan-cancer diagnosis been clarified. With assistance The Cancer Genome Atlas (TCGA) Gene Expression Omnibus (GEO), we have comprehensively mapped profiles, prognostic significance, genetic alteration, phosphorylation status, infiltration immune cells, functional properties 33 human...
The E3 ubiquitin ligase neural precursor cell-expressed developmentally down-regulated protein 4 (NEDD4) plays a crucial role in governing number of signaling pathways, including insulin and autophagy signaling. However, the molecular mechanism by which NEDD4 gene is transcriptionally regulated has not been fully elucidated. Here, we reported that mRNA levels were increased peroxisome proliferator-activated receptor-γ (PPARγ) HepG2 hepatocytes. PPARγ antagonist GW9662 abolished...
Pathological angiogenesis plays a major role in many disease processes, including cancer and diabetic retinopathy. Antiangiogenic therapy is potential management for pathologic angiogenesis. The novel synthetic compound 221S-1a, derived from captopril, tanshinol borneol, may have antiangiogenic properties. On the basis of MS, NMR HPLC analysis, structure 221S-1a was identified. cellular uptake metabolism this also observed. Next, properties were evaluated tumor-xenograft OIR models vivo....
Drug-induced liver injury (DILI), especially acetaminophen overdose, is the leading cause of acute failure. Pregnane X receptor (PXR) a nuclear and master regulator drug metabolism. Aberrant activation PXR plays pathogenic role in hepatotoxicity. Here, we aimed to examine S-nitrosylation (SNO) response acetaminophen. We found that was S-nitrosylated hepatocytes mouse livers after exposure or S-nitrosoglutathione (GSNO). Mass-spectrometry site-directed mutagenesis identified cysteine 307 as...