A5013977675- Developmental Biology and Gene Regulation
- MicroRNA in disease regulation
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Angiogenesis and VEGF in Cancer
- Epigenetics and DNA Methylation
- RNA Research and Splicing
- Immune cells in cancer
- Cancer-related gene regulation
- Liver physiology and pathology
- Neurogenesis and neuroplasticity mechanisms
- Cancer-related molecular mechanisms research
- Cancer, Hypoxia, and Metabolism
- Phagocytosis and Immune Regulation
- Immunotherapy and Immune Responses
- RNA modifications and cancer
- Cancer Cells and Metastasis
- Organ Transplantation Techniques and Outcomes
- Plant tissue culture and regeneration
- Mechanisms of cancer metastasis
- Genomics and Chromatin Dynamics
- Infectious Diseases and Mycology
- Circular RNAs in diseases
- Immune Response and Inflammation
- Axon Guidance and Neuronal Signaling
Lanzhou University
2024-2025
Lanzhou University Second Hospital
2024-2025
Shanxi University
2025
Heilongjiang University of Chinese Medicine
2025
Air Force Medical University
2015-2024
Xijing Hospital
2006-2024
Yangzhou University
2024
University of Chinese Academy of Sciences
2024
Chinese Academy of Sciences
2014-2024
Hebei University of Engineering
2024
The transcription factor recombination signal binding protein-J (RBP-J) functions immediately downstream of the cell surface receptor Notch and mediates transcriptional activation by intracellular domain all four kinds receptors. To investigate function RBP-J, we introduced loxP sites on both sides RBP-J exons encoding its DNA domain. Mice bearing loxP-flanked alleles, RBP-J(f/f), were mated with Mx-Cre transgenic mice deletional mutation gene in adult was induced injection IFN-alpha inducer...
Macrophages are important tumor-infiltrating cells and play pivotal roles in tumor growth metastasis. participate immune responses to tumors a polarized manner: classic M1 macrophages produce interleukin (IL) 12 promote tumoricidal responses, whereas M2 IL10 help progression. The mechanisms governing macrophage polarization unclear. Here, we show that the M2-like tumor-associated (TAM) have lower level of Notch pathway activation mouse models. Forced signaling increased which IL12, no matter...
Vascular integrity helps maintain brain microenvironment homeostasis, which is critical for the normal development and function of central nervous system. It known that neural cells can regulate vascular integrity. However, due to high complexity neurovascular interactions involved, understanding regulation still rudimentary. Using intact zebrafish larvae cultured rodent cells, we find neurons transfer miR-132, a highly conserved neuron-enriched microRNA, via secreting exosomes endothelial...
Abstract Tumor-associated macrophages (TAMs) are a major component of tumor microenvironment (TME) and play pivotal roles in the progression hepatocellular carcinoma (HCC). Wnt signaling is evolutionarily conserved participates liver tumorigenesis. Several studies have shown that macrophage-derived ligands can activate cells. However, whether secreted by cells trigger still elusive. In this study, we first verified canonical Wnt/β-catenin was activated during monocyte-to-macrophage...
Background The effectiveness of immune checkpoint inhibitors in colorectal cancer (CRC) is limited due to the low tumor neoantigen load and infiltration most microsatellite-stable (MSS) tumors. This study aimed develop a mitochondria-targeted photodynamic therapy (PDT) approach provoke host antitumor immunity MSS-CRC elucidate underlying molecular mechanisms. Methods role mechanism PDT inhibiting CRC progression inducing pyroptosis were evaluated both vitro vivo. effects sensitization on...
Melanoblasts (Mbs) are thought to be strictly regulated by cell–cell interactions with epidermal keratinocytes, although the precise molecular mechanism of regulation has been elusive. Notch signaling, whose activation is mediated interactions, implicated in a broad range developmental processes. We demonstrate vital role signaling maintenance Mbs, as well melanocyte stem cells (MSCs). Conditional ablation lineage leads severe defect hair pigmentation, followed intensive graying. The caused...
The most severe form of brain glioma, glioblastoma (GBM), is highly malignant and usually resistant to chemotherapy. Therefore, discovery new targets for gene therapy important. Using subtraction cloning, we identified the human N-Myc downstream-regulated 2 (hNDRG2), located at chromosome 14q11.2, as a that significantly suppressed in GBM tissues. Semiquantitative RT-PCR showed hNDRG2 transcript expressed normal tissue low-grade gliomas but present low levels 15 27 (56%) tissues all 6 cell...
Liver sinusoidal endothelial cells (LSECs) critically regulate liver homeostasis and diseases through angiocrine factors. Notch is critical in (ECs). In the current study, signaling was activated by inducible EC‐specific expression of intracellular domain (NIC). We found that activation damaged homeostasis. resulted decreased fenestration increased basement membrane, a gene profile with LSEC‐associated genes continuous EC‐associated genes, suggesting LSEC dedifferentiation. Consistently,...
The Notch pathway plays critical roles in the differentiation and polarized activation of macrophages; however, downstream molecular mechanisms underlying activity macrophages remain elusive. Our previous study has identified a group microRNAs that mediate signaling to regulate macrophage tumor-associated (TAMs). In this study, we demonstrated miR-148a-3p functions as novel molecule promote monocytes into presence granulocyte colony-stimulating factor (GM-CSF). Meanwhile, promoted M1...
Abstract Tumor-associated macrophages (TAM) contribute greatly to hallmarks of cancer. Notch blockade was shown arrest TAM differentiation, but the precise role and underlying mechanisms require elucidation. In this study, we employed a transgenic mouse model in which Notch1 intracellular domain (NIC) is activated conditionally define effects active signaling macrophages. NIC overexpression had no effect on it abrogated function, leading repressed growth transplanted tumors. Macrophage miRNA...
Abstract Tumor-associated macrophages (TAM) play pivotal roles in tumor progression and metastasis, but the contribution regulation of different macrophage populations remain unclear. Here we show that Notch signaling plays distinct regulating TAM subsets hepatocellular carcinoma (HCC). Myeloid-specific NOTCH blockade by conditional disruption recombination signal binding protein Jκ (RBPj cKO) significantly delayed growth subcutaneously inoculated Lewis lung (LLC), accelerated orthotopically...
Transforming growth factor-β (TGF-β) has received much attention as a major inducer of epithelial-mesenchymal transition (EMT) during cancer progression, mainly by activating set pleiotropic transcription factors including SNAI2/Slug.However, the involvement long non-coding RNAs (lncRNAs) in TGF-β-induced Slug activation and EMT remains largely unknown.Methods: In this study, we used microarray analysis to compare lncRNA expression profiles between TGF-β treated untreated breast cells.Then,...
The Notch pathway plays critical roles in the development and functional modulation of myeloid cells. Previous studies have demonstrated that activation promotes M1 polarization phagocytosis macrophages; however, downstream molecular mechanisms mediating signal remain elusive. In an attempt to identify targets bone marrow-derived macrophages (BMDMs) using mass spectrometry, regulatory protein α (SIRPα) appeared respond knockout recombination signal-binding Jk (RBP-J), transcription factor...
The mechanisms involved in liver regeneration after partial hepatectomy (pHx) are complicated. Cellular senescence, once linked to aging, plays a pivotal role wound repair. However, the regulatory effects of cellular senescence on have not been fully elucidated.Mice subjected pHx were analyzed 14 days surgery. incomplete remodeling sinusoids affected shear stress-induced endothelial nitric oxide synthase (eNOS) signaling day 14, resulting accumulation senescent LSECs. Removing macrophages...
Myeloid-derived suppressor cells (MDSCs) and tumor-associated macrophages (TAMs) play critical roles in tumorigenesis. However, the mechanisms underlying MDSC TAM development function remain unclear. In this study, we find that myeloid-specific activation of Notch/RBP-J signaling downregulates lactate transporter MCT2 transcription via its downstream molecule Hes1, leading to reduced intracellular levels, blunted granulocytic (G-MDSC) differentiation, enhanced maturation. We identify c-Jun...