A5102987446- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- Chromosomal and Genetic Variations
- Epigenetics and DNA Methylation
- Phagocytosis and Immune Regulation
- PI3K/AKT/mTOR signaling in cancer
- Erythrocyte Function and Pathophysiology
- Mast cells and histamine
- RNA Interference and Gene Delivery
- Immune Cell Function and Interaction
- RNA modifications and cancer
- T-cell and B-cell Immunology
- Animal Genetics and Reproduction
- Renal and related cancers
- Pancreatic function and diabetes
- RNA regulation and disease
- Cancer-related gene regulation
- Cancer and Skin Lesions
- Cell death mechanisms and regulation
- Cell Adhesion Molecules Research
- Polyamine Metabolism and Applications
- Reproductive Biology and Fertility
- Zebrafish Biomedical Research Applications
- Advanced biosensing and bioanalysis techniques
- Genetic Syndromes and Imprinting
Osaka University
2016-2025
Ube Frontier University
2007-2024
Kitakyushu Municipal Medical Center
2018-2024
Japan Agency for Medical Research and Development
2018-2019
Graduate School USA
2019
Japan Science and Technology Agency
2013-2018
Futaba (Japan)
2000-2016
Shionogi (Japan)
2001-2016
Osaka Medical and Pharmaceutical University
2010
Frontier Science Foundation
2007-2008
Silencing of transposable elements occurs during fetal gametogenesis in males via de novo DNA methylation their regulatory regions. The loss MILI (miwi-like) and MIWI2 (mouse piwi 2), two mouse homologs Drosophila Piwi, activates retrotransposon gene expression by impairing the regions retrotransposons. However, as it is unclear whether defective mutants due to impairment methylation, we analyze Piwi-interacting small RNA (piRNA) wild-type, MILI-null, MIWI2-null male germ cells. We reveal...
An efficient system was developed that induced the differentiation of embryonic stem (ES) cells into blood erythroid, myeloid, and B cell lineages by coculture with stromal line OP9. This does not express functional macrophage colony-stimulating factor (M-CSF). The presence M-CSF had inhibitory effects on ES to other than macrophages. Embryoid body formation or addition exogenous growth factors required, highly reproducible even after selection antibiotic G418. Combined ability genetically...
The piwi family genes, which are defined by conserved PAZ and Piwi domains, play important roles in stem cell self-renewal, RNA silencing, translational regulation various organisms. To reveal the function of mammalian homolog piwi, we produced analyzed mice with targeted mutations Mili gene, is one three mouse homologs piwi. Spermatogenesis MILI-null was blocked completely at early prophase first meiosis, from zygotene to pachytene, were sterile. However, primordial germ development female...
A method was developed to clone, without the use of specific functional assays, complementary DNAs (cDNAs) that carry amino-terminal signal sequences, such as those encoding intercellular signal-transducing molecules and receptors. The vector used in this system directed cell surface expression interleukin-2 receptor fusion proteins when inserts with sequences were cloned in-frame correct orientation. An cDNA library constructed from a bone marrow stromal line, which contained 5′...
PTEN is a tumor suppressor gene mutated in many human cancers, and its expression reduced or absent almost half of hepatoma patients. We used the Cre-loxP system to generate hepatocyte-specific null mutation Pten mice (AlbCrePtenflox/flox mice). AlbCrePtenflox/flox showed massive hepatomegaly steatohepatitis with triglyceride accumulation, phenotype similar nonalcoholic steatohepatitis. Adipocyte-specific genes were induced mutant hepatocytes, implying adipogenic-like transformation these...
ABSTRACT The Notch pathway functions in multiple cell fate determination processes invertebrate embryos, including the decision between neuroblast and epidermoblast lineages Drosophila. In mouse, targeted mutation of genes Notch1 RBP-Jk has demonstrated a role for these somite segmentation, but function neurogenesis decisions not been shown. Here we show that mutations lead to altered expression signalling homologues Hes-5, Mash-1 Dll1, resulting enhanced neurogenesis. Precocious neuronal...
Axl, Sky, and Mer, members of an Axl/Sky receptor tyrosine kinase subfamily, are typified by the cell adhesion molecule-related extracellular domain. The product growth arrest-specific gene 6 (Gas6), structurally homologous to anticoagulant protein S, was recently identified as ligand for Axl but Mer remained unknown. We have now obtained evidence that Gas6 can also function a Mer. Co-precipitation analysis, using soluble receptors (Axl-Fc, Sky-Fc, Mer-Fc) composed domain fused Fc...
Both connective tissue mast cells and grown in vitro are derived from multipotential hematopoietic stem cells, but these two cell populations exhibit many differences morphology, biochemistry, function. We investigated whether the phenotype of cultured or their progeny was altered when were transferred into different locations vivo. Cultured immature by ultrastructure, stained with alcian blue neither safranin berberine sulfate, a fluorescent dye that binds to heparin granules. By contrast,...
ABSTRACT The RBP-Jк protein is a transcription factor that recog-nizes the sequence C(T)GTGGGGA. gene highly conserved in wide variety of species and Drosophila homologue has been shown to be identical Suppressor Hairless [Su(H)] which plays important roles development peripheral nervous system. To explore function mouse embryo-genesis, mutation was introduced into functional embryonic stem (ES) cells by homologous recombination. Null mutant ES survived but null mice showed lethality before...
Genomic imprinting causes parental origin-specific monoallelic gene expression through differential DNA methylation established in the germ line. However, mechanisms underlying how specific sequences are selectively methylated not fully understood. We have found that components of PIWI-interacting RNA (piRNA) pathway required for de novo differentially region (DMR) imprinted mouse Rasgrf1 locus, but other paternally loci. A retrotransposon sequence within a noncoding spanning DMR was...
PTEN is an important tumor suppressor gene. Hereditary mutation of causes tumor-susceptibility diseases such as Cowden disease. We used the Cre-loxP system to generate endothelial cell-specific Pten ( Tie2CrePten ) in mice. flox/+ mice displayed enhanced tumorigenesis due increase angiogenesis driven by vascular growth factors. This effect was partially dependent on PI3K subunits p85 α and p110 γ. In vitro, cells showed proliferation/migration. flox/flox died before embryonic day 11.5...
Spermatogonial stem cells have unique properties to self-renew and support spermatogenesis throughout their lifespan. Although glial cell line-derived neurotrophic factor (GDNF) has recently been identified as a self-renewal for spermatogonial cells, the molecular mechanism of remains unclear. In present study, we assessed role phosphoinositide-3 kinase (PI3K)-Akt pathway using germline (GS) culture system that allows in vitro expansion cells. Akt was rapidly phosphorylated when GDNF added...