A5100426916- Cancer-related molecular mechanisms research
- Epigenetics and DNA Methylation
- MicroRNA in disease regulation
- Developmental Biology and Gene Regulation
- Angiogenesis and VEGF in Cancer
- Immune cells in cancer
- RNA modifications and cancer
- Cancer, Hypoxia, and Metabolism
- Cancer Cells and Metastasis
- Pluripotent Stem Cells Research
- Cancer-related gene regulation
- Histone Deacetylase Inhibitors Research
- Hydrogels: synthesis, properties, applications
- Ubiquitin and proteasome pathways
- Hematopoietic Stem Cell Transplantation
- Extracellular vesicles in disease
- Metabolomics and Mass Spectrometry Studies
- Bacteriophages and microbial interactions
- Immune Cell Function and Interaction
- Biomedical and Engineering Education
- Antibiotic Resistance in Bacteria
- Bladder and Urothelial Cancer Treatments
- Circular RNAs in diseases
- interferon and immune responses
- T-cell and B-cell Immunology
Guangdong Medical College
2023-2025
First Affiliated Hospital of Xi'an Jiaotong University
2012-2024
The People's Hospital of Guangxi Zhuang Autonomous Region
2024
Jiangsu Normal University
2024
Hebei Provincial Center for Disease Control and Prevention
2012-2024
Air Force Medical University
2014-2023
BeiGene (China)
2023
Zhejiang University
2008-2023
Jiangsu University
2023
Affiliated Hospital of Jiangsu University
2023
Macrophages are important tumor-infiltrating cells and play pivotal roles in tumor growth metastasis. participate immune responses to tumors a polarized manner: classic M1 macrophages produce interleukin (IL) 12 promote tumoricidal responses, whereas M2 IL10 help progression. The mechanisms governing macrophage polarization unclear. Here, we show that the M2-like tumor-associated (TAM) have lower level of Notch pathway activation mouse models. Forced signaling increased which IL12, no matter...
Liver sinusoidal endothelial cells (LSECs) critically regulate liver homeostasis and diseases through angiocrine factors. Notch is critical in (ECs). In the current study, signaling was activated by inducible EC‐specific expression of intracellular domain (NIC). We found that activation damaged homeostasis. resulted decreased fenestration increased basement membrane, a gene profile with LSEC‐associated genes continuous EC‐associated genes, suggesting LSEC dedifferentiation. Consistently,...
Mutations in the inositol 5-phosphatase OCRL cause Lowe syndrome and Dent's disease. Although OCRL, a direct clathrin interactor, is recruited to late-stage clathrin-coated pits, clinical manifestations have been primarily attributed intracellular sorting defects. Here we show that loss patient fibroblasts impacts clathrin-mediated endocytosis results an endocytic defect. These cells exhibit accumulation of vesicles increase U-shaped which may result from sequestration coat components on...
// Jiang-Hui Zeng 1, * , Liang 2, Rong-Quan He 3 Rui-Xue Tang 1 Xiao-Yong Cai 2 Jun-Qiang Chen 4 Dian-Zhong Luo Gang Department of Pathology, First Affiliated Hospital Guangxi Medical University, Nanning, Zhuang Autonomous Region, P. R. China General Surgery, University (West Branch), Oncology, Gastrointestinal These authors contributed equally to this work Correspondence to: Luo, email: 13878802796@163.com Chen, chen_gang_triones@163.com Keywords: lncRNA, COAD, READ, prognostic biomarker,...
In response to DNA damage, bacterial RecA protein forms filaments with the assistance of DinI protein. The stimulate autocleavage LexA, repressor more than 50 SOS genes, and activate response. During late phase response, UmuD λ CI, leading mutagenic repair lytic cycle, respectively. Here, we determined cryo-electron microscopy structures Escherichia coli in complex DinI, UmuD, λCI by helical reconstruction. reveal that LexA dimers bind filament groove cleave an intramolecular intermolecular...
Hepatic ischemia/reperfusion (I/R) injury is initiated by reactive oxygen species (ROS) accumulated during the early reperfusion phase after ischemia, but cellular mechanisms controlling ROS production and scavenging have not been fully understood. In this study, we show that blocking Notch signal knockout of transcription factor RBP-J or a pharmacological inhibitor led to aggravated hepatic I/R injury, as manifested deteriorated liver function increased apoptosis, necrosis, inflammation,...
Abstract Background Cancer stem cells (CSCs) play an important role in the development and recurrence of malignant tumors including glioma. Notch signaling, evolutionarily conserved pathway mediating direct cell-cell interaction, has been shown to regulate neural (NSCs) glioma (GSCs) normal neurogenesis pathological carcinogenesis, respectively. However, how signaling regulates proliferation differentiation GSCs not well elucidated. Methods We isolated cultivate human from patient specimens....
// Lei Li 1,2,* , Qiang Dang Hongjun Xie 1,2 Zhao Yang 1 Dalin He Liang Wenbing Song Shuyuan Yeh 2 Chawnshang Chang 2,3 Department of Urology, Sex Hormone Research Center, The First Affiliated Hospital, Xi’an Jiaotong University, Xi’an, China Departments Pathology and George Whipple Lab for Cancer Research, Wilmot University Rochester Medical Rochester, New York, USA 3 University/Hospital, Taichung, Taiwan * These authors have contributed equally to this work Correspondence to:...
KLF5 (Krüppel-like factor 5) plays critical roles in normal and cancer cell proliferation through modulating cycle progression. In this study, we demonstrated that curcumin targeted by promoting its proteasome degradation, but not inhibiting transcription bladder cells. We also lentivirus-based knockdown of inhibited growth, while over-expression a Flag-tagged could partially reverse the effects on growth cyclin D1 expression. Furthermore, found down-regulate expression Hippo pathway...
Abstract Extracellular vesicles (EVs) including exosomes can serve as mediators of cell–cell communication under physiological and pathological conditions. However, cargo molecules carried by EVs to exert their functions, well mechanisms for regulated release intake, have been poorly understood. In this study, we examined the effects endothelial cells-derived on neurons suffering from oxygen-glucose deprivation (OGD), which mimics neuronal ischemia-reperfusion injury in human diseases. a...
This paper reports a thermosensitive polymer−clay nanocomposite with enhanced temperature response based on organically modified clay−poly(N-isopropylacrylamide) (PNIPAAm) nanocomposites. Compared previously reported nanocomposites, the interfacial chemistry between clay minerals and polymer in new is through coupling agent to favor more efficient thermal transition. material exhibits an unusually large volumetric change faster transition kinetics as compared conventional PNIPAAm gels. These...
Temperature-sensitive surfaces were prepared by grafting poly(N-isopropylacrylamide) (PNIPAAm) hydrogel on the surface of silicone wafers. The wafer substrates modified organosilane (vinyltriethoxylsilane). They further reacted with N-isopropylacrylamide (NIPAAm), using N,N'-methylenebisacrylamide as cross-linking agent, to generate cross-linked PNIPAAm layer substrate. characterized scanning electron microscopy, X-ray photoelectron spectroscopy, and attenuated total reflectance−Fourier...
Because extracellular matrix metalloproteinase inducer (EMMPRIN), a tumor cell-derived protein, induces metalloproteinases (MMPs) in fibroblasts and because MMPs are important atheroma formation, we investigated if EMMPRIN was expressed granulocyte/macrophage-colony stimulating factor (GM-CSF)-differentiated human peripheral blood monocytes (HPBM) macrophage foam cells. In addition, studied for its expression atheroma.After 10 days of GM-CSF-induced monocyte differentiation, mRNA increased...
Background Endothelial cells ( EC s) form blood vessels through angiogenesis that is regulated by coordination of vascular endothelial growth factor VEGF ), Notch, transforming β, and other signals, but the detailed molecular mechanisms remain unclear. Methods Results Small RNA sequencing initially identified miR‐342‐5p as a novel downstream molecule Notch signaling in s. Reporter assay, quantitative reverse transcription polymerase chain reaction Western blot analysis indicated targeted...
Ocular neovascularization is a comprehensive process involved in retinal vascular development and several blinding diseases such as age-related macular degeneration retinopathy of prematurity, with endothelial growth factor (VEGF) regarded the master regulator. However, qualified effect anti-VEGF therapy reveals that underlying mechanisms are still not clearly identified. To initialize angiogenesis, cells undergo phenotype switching to generate highly migratory invasive cells. This shares...
Abnormal metabolism serves a critical role in the development and progression of different types malignancies including glioblastoma (GBM), may therefore serve as promising target for treatment cancer. Preclinical studies have indicated that ketogenic diet (KD) exhibit beneficial effects patients with GBM; however, underlying mechanisms remain incompletely understood. The aim present study was to evaluate KD on glioma stem‑like cells (GSCs), by culturing patient‑derived primary GSCs well GSC...
Abstract Background Glioma stem cells (GSCs) are glioma with stemness and responsible for a variety of malignant behaviors glioma. Evidence has shown that signals from tumor microenvironment (TME) enhance cells. However, identification the signaling molecules underlying mechanisms not been completely elucidated. Methods Human samples cell lines were cultured in vitro to determine effects adenovirus (ADV) infection by sphere formation, RT-qPCR, western blotting, FACS immunofluorescence. For...
Melatonin helps to maintain circadian rhythm, exerts anticancer activity, and plays key roles in regulation of glucose homeostasis energy metabolism. Glycosylation, a form metabolic flux from or other monosaccharides, is common post-translational modification. Dysregulated glycosylation, particularly O-GlcNAcylation, often biomarker cancer cells. In this study, elevated O-GlcNAc level bladder was inhibited by melatonin treatment. treatment proliferation migration enhanced apoptosis Proteomic...
Ferroptosis is involved in the occurrence and development of inflammatory arthritis. RND1 has been reported to possess pro-ferroptosis activity. This study was designed explore role molecular mechanism rheumatoid arthritis (RA). DBA/1 mice were exposed type II collagen immunization. The pathological damage knee joints observed with H&E staining expression synovial tissues detected using Western blot. In vitro, blot used measure RND1, ferroptosis-, migration- inflammation-related proteins....
Formation of glioma stem cells (GSCs) is considered as one the main reasons temozolomide (TMZ) resistance in patients. Recent studies have shown that tumor microenvironment-derived signals could promote GSCs formation. But critical molecule and underlying mechanism for formation after TMZ treatment not entirely identified. Our study showed promoted by cells; biopsy-derived glioblastoma multiforme upregulated HMGB1; HMGB1 altered gene expression profile with respect to mRNA, lncRNA miRNA....