A5045315434- Developmental Biology and Gene Regulation
- Glycosylation and Glycoproteins Research
- Click Chemistry and Applications
- Monoclonal and Polyclonal Antibodies Research
- Bacterial Infections and Vaccines
- Angiogenesis and VEGF in Cancer
- Immune Cell Function and Interaction
- Epigenetics and DNA Methylation
- T-cell and B-cell Immunology
- MicroRNA in disease regulation
- Immune cells in cancer
- Plant biochemistry and biosynthesis
- Circular RNAs in diseases
- Wnt/β-catenin signaling in development and cancer
- Immune Response and Inflammation
- Cancer-related molecular mechanisms research
- Invertebrate Immune Response Mechanisms
- Infectious Encephalopathies and Encephalitis
- Metabolism and Genetic Disorders
- RNA modifications and cancer
- Atherosclerosis and Cardiovascular Diseases
- Spinal Cord Injury Research
- Advanced biosensing and bioanalysis techniques
- Spinal Dysraphism and Malformations
- Liver physiology and pathology
Yunnan Agricultural University
2022-2025
Tianjin Medical University General Hospital
2025
China International Science and Technology Cooperation
2025
Air Force Medical University
2008-2024
Shandong Center for Disease Control and Prevention
2014-2024
Guizhou University
2024
University of Chinese Academy of Sciences
2022
Shanghai Institute of Materia Medica
2020-2022
Chinese Academy of Sciences
2015-2022
Shandong University
2014-2021
Macrophages are important tumor-infiltrating cells and play pivotal roles in tumor growth metastasis. participate immune responses to tumors a polarized manner: classic M1 macrophages produce interleukin (IL) 12 promote tumoricidal responses, whereas M2 IL10 help progression. The mechanisms governing macrophage polarization unclear. Here, we show that the M2-like tumor-associated (TAM) have lower level of Notch pathway activation mouse models. Forced signaling increased which IL12, no matter...
Raising the bar: The efficacy of bioorthogonal reactions for bioconjugation has been thoroughly evaluated in four different biological settings. Powered by development new biocompatible ligands, copper-catalyzed azide–alkyne cycloaddition (see picture) brought about unsurpassed efficiency, and thus it holds great promise as a highly potent adaptive tool broader spectrum applications. Detailed facts importance to specialist readers are published ”Supporting Information”. Such documents...
Abstract The advent of click chemistry has had a profound impact on many fields and fueled need for reliable reactions to expand the toolkit. However, developing new systems fulfill criteria remains highly desirable yet challenging. Here, we report development light-induced primary amines o-nitrobenzyl alcohols cyclization (PANAC) as photoclick reaction via direct handle, rapid modular functionalization diverse small molecules native biomolecules. With intrinsic advantages temporal control,...
To monitor the kinetics of biological processes that take place within minute time scale, simple and fast analytical methods are required. In this article, we present our discovery an azide with internal Cu(I)-chelating motif enabled development fastest protocol for Cu(I)-catalyzed azide–alkyne cycloaddition (CuAAC) to date, its application toward following dynamic process glycan biosynthesis. We discovered electron-donating picolyl boosted efficiency ligand-accelerated CuAAC 20–38-fold in...
Background Accumulating evidence has shown that tumor-associated macrophages (TAMs) play a critical role in tumor progression. Targeting TAMs is potential strategy for immunotherapy. However, the mechanism underlying TAM phenotype and function needs to be resolved. Our previous studies have demonstrated miR-125a can reverse toward antitumor. Meanwhile, we found miR-99b cluster first intron of same host gene, are transcribed simultaneously bone marrow-derived (BMDMs) following LPS+IFNγ...
Knee osteoarthritis (KOA) is a major cause of leg disability in the elderly population. Recently, expression levels circulating microRNA (miRNA) let‑7e have been reported to be significantly reduced KOA. The aims present study were assess feasibility as serum marker for detecting KOA and explore underlying mechanisms its involvement. Based on previous studies bioinformatics analysis, may regulate apoptosis autophagy articular chondrocytes. A total 10 patients with trauma without recruited...
Neue Maßstäbe: Die Wirksamkeit bioorthogonaler Reaktionen für die Biokonjugation wurde an vier biologischen Systemen getestet. Mit der Einführung neuer biokompatibler Liganden sind Kupfer-katalysierte Azid-Alkin-Cycloadditionen (siehe Schema) mit bisher unerreichter Biokonjugationseffizienz möglich, was Klick-Reaktion zu einer hoch wirksamen Methode ein breites Spektrum biologischer Anwendungen macht.
Insufficient endometrial receptivity is a major factor leading to implantation failure (IF), and the traditional way of morphological observation endometrium cannot determine condition sufficiently. Considering that long-noncoding RNAs (lncRNAs) regulate competing endogenous RNA (ceRNA) mechanism works in plenty biological processes, ceRNA likely function pathology IF. In present study, we aim construct an related lncRNA-mRNA network (IFLMN), identify key lncRNAs as candidates for predicting...
Tartaric acid (TA) is the primary organic present in grapes and a fundamental constituent of wine, responsible for shaping its taste, aroma, overall quality. This review presents comprehensive overview advances made previous investigations on grape tartaric acid. It elucidates structural properties, distribution characteristics, biosynthesis, catabolism, transcriptional regulation acid, also speculates regulatory mechanism based modulation ascorbic acid-related transcription factors....
To analyze the epidemiological characteristics and clinical features of pertussis cases reported in Shandong Province China.
Abstract Centella asiatica is renowned for its medicinal properties, particularly due to triterpenoid saponins, such as asiaticoside and madecassoside, which are in excess demand the cosmetic industry. However, comprehensive genomic resources this species lacking, impedes understanding of biosynthetic pathways. Here, we report a telomere-to-telomere (T2T) C. genome. The genome size 438.12 Mb with contig N50 length 54.12 Mb. comprises 258.87 repetitive sequences 25,200 protein-coding genes....
Macrophages play multidimensional roles in hepatic fibrosis, but their control has not been fully understood. The Notch pathway mediated by recombination signal binding protein Jκ (RBP‐J), the transcription factor transactivated signals from four mammalian receptors, is implicated macrophage activation and plasticity. In this study, using mouse fibrosis models, we show that myeloid‐specific disruption of RBP‐J resulted attenuated fibrosis. stellate cells production profibrotic factors...
Various mechanisms have been identified by which viruses target host small RNA biogenesis pathways to achieve optimal infection outcomes. Herpes simplex virus 1 (HSV-1) is a ubiquitous human pathogen whose successful persistence in the entails both productive (“lytic”) and latent infection. Although many HSV-1 miRNAs discovered some are thought help control lytic/latent switch, little known about regulation of their biogenesis. By characterizing expression pre-miRNAs mature under various...
To develop a safe and reproducible method for harvesting viable vascular endothelium to analyze gene expression at sites of lesions.Coaxial curved stainless-steel guide wires were used obtain samples endothelial cells from large arteries veins in 29 patients undergoing routine endovascular procedures. Three immunocytochemical markers identify as endothelial. Cellular viability was evaluated terms cell membrane integrity, energy-dependent uptake acetylated low-density lipoprotein, cellular...
Yolk sac–derived microglia and peripheral monocyte–derived macrophages play a key role during Parkinson’s disease (PD) progression. However, the regulatory mechanism of microglia/macrophage activation function in PD pathogenesis remains unclear. Recombination signal–binding protein Jκ (RBP-J)–mediated Notch signaling regulates macrophage development activation. In this study, with an 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) hydrochloride-induced acute murine model, we found that...
Introduction of a nitrogen containing group as hydrogen bond acceptor is useful strategy for the structure modification.
The DNA‐binding protein recombination signal‐binding protein‐Jk (RBP‐J) plays a key role in transcriptional regulation by targeting the intracellular domain of Notch (NIC) and Epstein–Barr virus nuclear antigen 2 (EBNA2) to specific promoters. In absence signaling, RBP‐J acts as suppressor through recruiting co‐suppressors such histone deacetylase (HDAC). KyoT2 is LIM that suppresses RBP‐J‐mediated activation. current study, we show polycomb group (PcG) HPC2, which functions suppressor,...