A5014338515- Herpesvirus Infections and Treatments
- RNA regulation and disease
- interferon and immune responses
- Cytomegalovirus and herpesvirus research
- Photodynamic Therapy Research Studies
- Porphyrin and Phthalocyanine Chemistry
- Animal Virus Infections Studies
- MicroRNA in disease regulation
- Toxoplasma gondii Research Studies
- Helicobacter pylori-related gastroenterology studies
- Eosinophilic Esophagitis
- Viral Infections and Immunology Research
- Nanoplatforms for cancer theranostics
- Oral microbiology and periodontitis research
- Neuroinflammation and Neurodegeneration Mechanisms
- Alzheimer's disease research and treatments
- Circular RNAs in diseases
- Virus-based gene therapy research
- RNA Interference and Gene Delivery
- FOXO transcription factor regulation
- Porphyrin Metabolism and Disorders
- SARS-CoV-2 and COVID-19 Research
- Viral-associated cancers and disorders
- Medical and Biological Ozone Research
- Cancer-related molecular mechanisms research
University of Rijeka
2017-2025
Harvard University
2008-2019
Robert Koch Institute
2006-2010
Universität Hamburg
2010
Leibniz Institute of Virology (LIV)
2010
University of Würzburg
2006
Rudjer Boskovic Institute
2001
Abstract Since the original publication of sRNAtoolbox in 2015, small RNA research experienced notable advances different directions. New protocols for sequencing have become available to address important issues such as adapter ligation bias, PCR amplification artefacts or include internal controls spike-in sequences. microRNA reference databases were developed with foci, either prioritizing accuracy (low number false positives) completeness negatives). Additionally, other molecules well...
Certain viruses use microRNAs (miRNAs) to regulate the expression of their own genes, host or both. Previous studies have identified a limited number miRNAs expressed by herpes simplex 1 and 2 (HSV-1 -2), some which are conserved between these two viruses. To more comprehensively analyze HSV-1 HSV-2 during productive latent infection, we applied massively parallel sequencing approach. We were able identify 16 17 HSV-2, respectively, including all previously known species, unidentified...
ABSTRACT Intrinsic immunity is a first-line intracellular defense against virus infection, and viruses have evolved mechanisms to counteract it. During herpes simplex (HSV) nuclear domain 10 (ND10) components localize adjacent incoming viral genomes generate repressive environment for gene expression. Here, we found that the ND10 component, alpha-thalassemia/mental retardation syndrome X-linked (ATRX) protein, predicted be target of HSV-1 miR-H1 HSV-2 miR-H6. These microRNAs (miRNAs) share...
Many of the potential immune therapeutic targets are similarly affected in adult-onset neurodegenerative diseases such as Alzheimer’s (AD) disease, Parkinson’s disease (PD), amyotrophic lateral sclerosis (ALS), and frontotemporal dementia (FTD), but also a seemingly distinct Niemann-Pick type C with primarily juvenile-onset. This strongly argues for an overlap pathogenic mechanisms. The commonly researched include various cell subsets microglia, peripheral macrophages, or regulatory T cells...
Adenosine deaminase acting on dsRNA 1 (ADAR1) catalyzes the deamination of adenosines to inosines in double-stranded RNAs (dsRNA) and regulates innate immunity by preventing hyperactivation cytosolic sensors such as MDA5, PKR or ZBP1. ADAR1 has been shown exert pro- antiviral, editing-dependent editing-independent functions viral infections, but little is known about its function herpesvirus replication. We now demonstrate that herpes simplex virus (HSV-1) hyperactivates absence ADAR1,...
Nested polymerase chain reaction (PCR) was performed to detect the presence of Helicobacter pylori in tongue mucosa 268 patients divided into four groups according their diagnosis: 87 with atrophic glossitis, 37 benign migratory glossitis and 144 burning mouth syndrome (BMS). The latter group subdivided anatomic site sensation: subgroup A (54 patients) complaints limited B (90 sensations other parts oral mucosa. H. found 43 samples (16%). Bacteria were significantly less present affected...
Various mechanisms have been identified by which viruses target host small RNA biogenesis pathways to achieve optimal infection outcomes. Herpes simplex virus 1 (HSV-1) is a ubiquitous human pathogen whose successful persistence in the entails both productive (“lytic”) and latent infection. Although many HSV-1 miRNAs discovered some are thought help control lytic/latent switch, little known about regulation of their biogenesis. By characterizing expression pre-miRNAs mature under various...
ABSTRACT Many viruses encode proteins that inhibit the induction of programmed cell death at mitochondrial checkpoint. Murine cytomegalovirus (MCMV) encodes m38.5 protein, which localizes to mitochondria and protects human HeLa cells fibroblasts from apoptosis triggered by proteasome inhibitors but not Fas-induced apoptosis. However, ability this protein suppress murine its role during MCMV infection have been investigated previously. Here we show is expressed early time points infection....
To facilitate studies of herpes simplex virus 1 latency, cell culture models quiescent or latent infection have been developed. Using deep sequencing, we analyzed the expression viral microRNAs (miRNAs) in two employing human fibroblasts and one using rat neurons. In all cases, patterns differed from that productively infected cells, with neuron pattern most closely resembling found latently mouse ganglia vivo.
Several different families of DNA viruses encode proteins that inactivate the cellular retinoblastoma tumor suppressor protein (pRb), which normally functions to bind E2F transcription factors and restrict expression genes necessary for processes including replication. Human cytomegalovirus (HCMV) UL97, a kinase functionally orthologous cyclin-dependent kinases, phosphorylates pRb on inactivating residues during HCMV infection. To assess if such phosphorylation is biologically relevant, we...
ABSTRACT Cytomegaloviruses, representatives of the Betaherpesvirinae , cause opportunistic infections in immunocompromised hosts. They infect various cells and tissues their natural host but are highly species specific. For instance, human cytomegalovirus (HCMV) does not replicate mouse cells, permissive for murine (MCMV) infection. However, underlying molecular mechanisms so far poorly understood. In present study we isolated characterized a spontaneously occurring MCMV mutant that has...
Photodynamic therapy (PDT) is broadly used to treat different tumors, and it a rapidly developing approach inactivating or inhibiting the replication of fungi, bacteria, viruses. Herpes simplex virus 1 (HSV-1) an important human pathogen frequently model study effects PDT on enveloped Although many photosensitizers (PSs) have been tested for their antiviral properties, analyses are usually limited assessing reduction in viral yield, thus molecular mechanisms photodynamic inactivation (PDI)...
Infection with herpes simplex virus-1 (HSV-1) brings numerous changes in cellular gene expression. Levels of most host mRNAs are reduced, limiting synthesis proteins, especially those involved antiviral defenses. The impact HSV-1 on microRNAs (miRNAs), an extensive network short non-coding RNAs that regulate mRNA stability/translation, remains largely unexplored. Here we show transcription the miR-183 cluster (miR-183, miR-96, and miR-182) is selectively induced by during productive...
Recently, we have described the first human case of AdoHcyase (S-adenosylhomocysteine hydrolase) deficiency. Two point mutations in gene, missense mutation p.Y143C (AdoHcyase which Tyr143 is replaced by cysteine) and truncation p.W112stop Trp112 opal stop codon) were identified [Barić, Fumić, Glenn, Ćuk, Schulze, Finkelstein, James, Mejaški-Bošnjak, Pažanin, Pogribny et al. (2004) Proc. Natl. Acad. Sci. U.S.A. 101, 4234–4239]. To elucidate molecular catalytic properties AdoHcyase, made...
Herpes simplex virus 1 is an important human pathogen that has been intensively studied for many decades. Nevertheless, the molecular mechanisms regulating its establishment, maintenance, and reactivation from latency are poorly understood. Here, we show HSV-1-encoded miR-H2 post-transcriptionally edited in latently infected tissues. Hyperediting of viral miRNAs increases targeting potential these may play role latency. We can target ICP4, essential protein. Interestingly, found no evidence...
Background and Objectives: Herpes simplex keratitis (HSK) is the leading infectious cause of corneal damage associated loss visual acuity. Because its frequent recurrence, it represents a major health problem; thus, timely accurate diagnosis key to successful treatment. To enable this, we aimed determine HSK patients’ demographic clinical features. Materials Methods: This prospective study included 55 patients diagnosed with between March 2019 August 2022 at Department Ophthalmology,...