A5100382363- Plant Gene Expression Analysis
- Horticultural and Viticultural Research
- Plant Pathogens and Fungal Diseases
- Plant-Microbe Interactions and Immunity
- Plant Stress Responses and Tolerance
- Plant Disease Resistance and Genetics
- Wheat and Barley Genetics and Pathology
- Fermentation and Sensory Analysis
- Plant pathogens and resistance mechanisms
- Plant Reproductive Biology
- Plant Molecular Biology Research
- Plant biochemistry and biosynthesis
- Genetic Mapping and Diversity in Plants and Animals
- Plant tissue culture and regeneration
- Photosynthetic Processes and Mechanisms
- Mycorrhizal Fungi and Plant Interactions
- Insect Resistance and Genetics
- Plant Pathogens and Resistance
- Plant responses to water stress
- Soybean genetics and cultivation
- Fungal Biology and Applications
- Virus-based gene therapy research
- HIV Research and Treatment
- RNA modifications and cancer
- Plant Virus Research Studies
Northwest A&F University
2016-2025
Sichuan Academy of Agricultural Sciences
2019-2025
Sichuan Agricultural University
2016-2025
Ministry of Agriculture and Rural Affairs
2025
Nanjing Agricultural University
2025
Shanghai University of Traditional Chinese Medicine
2024
Jilin Academy of Agricultural Sciences
2020-2024
Guizhou University
2024
Inner Mongolia Agricultural University
2024
Southwest Forestry University
2023
The clustered regularly interspaced short palindromic repeats-associated protein 9 (CRISPR/Cas9) system is a powerful tool for editing plant genomes. Efficient genome of grape (Vitis vinifera) suspension cells using the type II CRISPR/Cas9 has been demonstrated; however, it not established whether this can be applied to get biallelic mutations in first generation grape. In current study, we designed four guide RNAs VvWRKY52 transcription factor gene with system, and obtained transgenic...
For the past decade, cancer genomic studies have focused on mutations leading to splice-site disruption, overlooking those having splice-creating potential. Here, we applied a bioinformatic tool, MiSplice, for large-scale discovery of splice-site-creating (SCMs) across 8,656 TCGA tumors. We report 1,964 originally mis-annotated clear evidence creating alternative splice junctions. TP53 and GATA3 26 18 SCMs, respectively, ATRX has 5 from lower-grade gliomas. Mutations in 11 genes, including...
Abstract Multiple cotton genomes (diploid and tetraploid) have been assembled. However, genomic variations between cultivars of allotetraploid upland ( Gossypium hirsutum L.), the most widely planted species in world, remain unexplored. Here, we use single-molecule long read Hi-C sequencing technologies to assemble two TM-1 zhongmiansuo24 (ZM24). Comparisons among ZM24 assemblies diploid ancestors reveal a large amount genetic variations. Among them, top three longest structural are located...
Abstract Powdery mildew (PM), caused by the fungal pathogen Erysiphe necator, is one of most destructive diseases grapevine (Vitis vinifera and other Vitis spp.). Resistance to PM an important goal for cultivar improvement, understanding underlying molecular mechanisms conditioning resistance critical. Here, we report that transgenic expression WRKY transcription factor gene VqWRKY31 from PM-resistant species quinquangularis conferred in V. through promoting salicylic acid signaling specific...
Thousands of long noncoding RNAs (lncRNAs) have been identified in mouse, rat, and human testes, some which play important roles testis development spermatogenesis. However, systematic analysis lncRNAs expressed postnatal pig testes has not reported. Thus, this study, we present the expression characterization immature (30-day-old [D30]) mature (180-day-old [D180]) testes. A total 90 440 168 (85.75%) 97 001 700 (95.35%) 150-base-pair paired-end clean reads were generated D30 D180 cDNA...
Drought stress severely affects grapevine quality and yield, recent reports have revealed that lignin plays an important role in protection from drought stress. Since little is known about lignin-mediated resistance grapevine, we investigated its significance. Herein, show VlbZIP30 mediates by activating the expression of biosynthetic genes increasing deposition. Transgenic plants overexpressing exhibited deposition (mainly G S monomers) stem secondary xylem under control conditions, which...
Summary Powdery mildew (PM) is a severe fungal disease of cultivated grapevine world‐wide. Proanthocyanidins (PAs) play an important role in resistance to pathogens; however, little known about PA‐mediated PM grapevine. We identified WRKY transcription factor, VqWRKY56 , from Vitis quinquangularis the expression which was significantly induced by PM. Overexpression (OE) vinifera increased PA content and reduced susceptibility Furthermore, transgenic plants showed more cell death accumulation...
Abstract Botrytis cinerea is a fungus that infects cultivated grape ( Vitis vinifera ); the identification and characterization of resistance mechanisms in host great importance for industry. Here, we report transcription factor ethylene‐responsive (ERF) family VaERF16 ) from Chinese wild amurensis ‘Shuang You’) expressed during B . infection response to treatments with hormones ethylene methyl jasmonate. Heterologous overexpression Arabidopsis thaliana substantially enhanced bacterium...
Photothermal therapy (PTT) is a promising non-invasive treatment that has shown great potential in eliminating tumors. It not only induces apoptosis of cancer cells but also triggers immunogenic cell death (ICD) which could activate the immune system against cancer. However, immunosuppressive tumor microenvironment (TIME) poses challenge to triggering strong responses with single treatment, thus limiting therapeutic effect immunotherapy. In this study, dual-targeted nano delivery (GOx@FeNPs)...
Abstract Ambient pH performs a crucial role in plant immunity, abiotic stress response, and growth development. Plant–pathogen interactions influence dynamic environment. Host acidification alkalinization are under the regulating actions of acidophilic alkaliphilic pathogens, respectively. Plasma membrane H + -ATPases regulate homeostasis, their activity is modulated during pathogen-associated molecular pattern- (PTI) effector-triggered immunities (ETI). Recent studies have provided insights...
Gibberellins are well known for their growth control function in flower, fruit and seed development, as such, exogenous gibberellic acid (GA) application plays an important role viticulture. Unfortunately, the mechanism by which GA3 acts regulation of these complicated developmental processes grape remains unclear. In present study, we demonstrated that to 'Kyoho' grapevine inflorescences at pre-bloom promoted flower opening, induced coloring abortion. attempt obtain a deeper understanding...
Gram-negative bacteria of the genus Serratia are potential producers many useful secondary metabolites, such as prodigiosin and serrawettins, which have applications in environmental bioremediation or pharmaceutical industry. Several strains produce serrawettin W1 main bioactive compounds, biosynthetic pathways co-regulated by quorum sensing (QS). In contrast, strain, can simultaneously W2, has not been reported. This study focused on analyzing genomic sequence sp. strain YD25T isolated from...
Abstract The CRISPR (clustered regularly interspaced short palindromic repeats)-associated protein 9 (Cas9) system is a powerful tool for targeted genome editing, with applications that include plant biotechnology and functional genomics research. However, the specificity of Cas9 targeting poorly investigated in many species, including fruit trees. To assess off-target mutation rate grapevine ( Vitis vinifera) , we performed whole-genome sequencing (WGS) seven Cas9-edited plants which one...
<h3>Importance</h3> Gastric neuroendocrine carcinoma and mixed adenoneuroendocrine are rare pathological types of gastric cancer, there is a lack multicenter studies comparing the prognosis recurrence patterns carcinoma, adenocarcinoma. <h3>Objective</h3> To compare differences in long-term survival among <h3>Design, Setting, Participants</h3> This cohort study included patients with resectable at 23 hospitals China from January 2006 to December 2016. In addition, adenocarcinoma were...
The Actinidia (kiwifruit) is an emerging fruit plant that severely affected by salt stress in northern China. Plants have evolved several signaling network mechanisms to cope with the detrimental effects of stress. To date, no reported work available on metabolic and molecular involved kiwifruit tolerance. Therefore, present study aims decipher intricate adaptive responses two contrasting tolerance species valvata [ZMH (an important genotype), hereafter referred as R] deliciosa ['Hayward'...
Plant essential oils contain many secondary metabolites, some of which can effectively inhibit the growth pathogenic microorganisms, so it is a very promising antibacterial agent. In this study, qualitative and quantitative method based on high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) was developed for simultaneous determination three bioactive substances, cinnamaldehyde (CNM), thymol (THY), eugenol (EUG), in plants. Necessary tests linearity, limit...
WRKY transcription factors are known to play important roles in plant responses various abiotic and biotic stresses. The grape gene, WRKY3 was previously reported respond salt drought stress, as well methyl jasmonate ethylene treatments Vitis labrusca × V. vinifera cv. 'Kyoho.' In the current study, from 'Kyoho' cultivar constitutively expressed Arabidopsis thaliana under control of cauliflower mosaic virus 35S promoter. 35S::VlWRKY3 transgenic A. plants showed improved stress tolerance...
Seedlessness in grape (Vitis vinifera) is of considerable commercial importance for both the table and processing industries. Studies to date seed development have been made certain progress, but many key genes yet be identified characterized. In this study we analyzed transcriptomes progeny derived from V. vinifera seeded maternal parent 'Red Globe' seedless paternal 'Centennial seedless' identify associated with seedlessness. A total 6,607 differentially expressed (DEGs) were examined...
Abstract Botrytis cinerea is a major grapevine ( Vitis spp.) pathogen, but some genotypes differ in their degree of resistance. For example, the vinifera cultivar Red Globe (RG) highly susceptible, V. amurensis Rupr Shuangyou (SY) resistant. Here, we used RNA sequencing analysis to characterize transcriptome responses these two B. inoculation at an early infection stage. Approximately quarter genes RG presented significant changes transcript levels during infection, number which was greater...
Seedlessness is one of the most important breeding goals for table grapes; thus, understanding molecular genetic regulation seed development and abortion critical seedless cultivars. In present study, we characterized VvMADS39, a class E MADS-box gene grapevine (Vitis vinifera) orthologous to Arabidopsis SEP2. Heterologous overexpression VvMADS39 in tomato reduced fruit size number. Targeted mutagenesis homologous SlMADS39 induced various floral defects. It could reasonable suppose that...