A5085040299- Research in Cotton Cultivation
- Plant Molecular Biology Research
- Plant tissue culture and regeneration
- Plant Stress Responses and Tolerance
- Plant-Microbe Interactions and Immunity
- Plant Virus Research Studies
- Plant Reproductive Biology
- Plant Gene Expression Analysis
- Photosynthetic Processes and Mechanisms
- CRISPR and Genetic Engineering
- Plant and Fungal Interactions Research
- Plant pathogens and resistance mechanisms
- RNA regulation and disease
- Silk-based biomaterials and applications
- Chromosomal and Genetic Variations
- Biofuel production and bioconversion
- Plant Micronutrient Interactions and Effects
- Plant Disease Resistance and Genetics
- Genetic Mapping and Diversity in Plants and Animals
- Plant nutrient uptake and metabolism
- Acoustic Wave Resonator Technologies
- Nuclear Receptors and Signaling
- Osteoarthritis Treatment and Mechanisms
- Light effects on plants
- Seed Germination and Physiology
Cotton Research Institute
2016-2025
Chinese Academy of Agricultural Sciences
2016-2025
Zhengzhou University
2019-2025
Soochow University
2022-2025
First Affiliated Hospital of Soochow University
2022-2025
Henan Normal University
2021-2024
Xinjiang Academy of Agricultural and Reclamation Science
2024
Fifth Affiliated Hospital of Zhengzhou University
2023
Southeast University
2023
State Key Laboratory of Cotton Biology
2018-2022
Abstract Multiple cotton genomes (diploid and tetraploid) have been assembled. However, genomic variations between cultivars of allotetraploid upland ( Gossypium hirsutum L.), the most widely planted species in world, remain unexplored. Here, we use single-molecule long read Hi-C sequencing technologies to assemble two TM-1 zhongmiansuo24 (ZM24). Comparisons among ZM24 assemblies diploid ancestors reveal a large amount genetic variations. Among them, top three longest structural are located...
Abstract Brassinosteroid (BR), a growth-promoting phytohormone, regulates many plant growth processes including cell development. However, the mechanism by which BR fiber is poorly understood. Cotton (Gossypium hirsutum) fibers are an ideal single-cell model in to study elongation due their length. Here we report that controls cotton modulating very-long-chain fatty acid (VLCFA) biosynthesis. deficiency reduces expression of 3-ketoacyl-CoA synthases (GhKCSs), rate-limiting enzymes involved...
MYB transcription factors (TFs) are one of the largest families TFs in higher plants and involved diverse biological, functional, structural processes. Previously, very few functional validation studies on R2R3 have been conducted cotton response to abiotic stresses. In current study, GaMYB85, a TF, was ectopically expressed Arabidopsis thaliana (Col-0) functionally characterized by overexpression transgenic plants. The in-silico analysis GaMYB85 shows presence SANT domain with conserved...
Gossypium hirsutum is an allotetraploid species, meaning that mutants are difficult to generate by classical approaches due gene redundancy. The CRISPR/Cas9 genome editing system a robust and highly efficient tool for generating target mutants, which the genes of interest may be functionally dissected applied through genotype-to-phenotype approaches. In this study, was developed in G. Gh14-3-3d gene. T0 transgenic plants, lots insertions deletions (indels) at expected site were detected...
WUSCHEL-related homeobox (WOX) family members play significant roles in plant growth and development, such as embryo patterning, stem-cell maintenance, lateral organ formation. The recently published cotton genome sequences allow us to perform comprehensive genome-wide analysis characterization of WOX genes cotton. In this study, we identified 21, 20, 38 Gossypium arboreum (2n = 26, A2), G. raimondii D5), hirsutum 4x 52, (AD)t), respectively. Sequence logos showed that domains were...
Branching determines cotton architecture and production, but the underlying regulatory mechanisms remain unclear. Here, we report that miR164-GhCUC2 (CUP-SHAPED COTYLEDON2) module regulates lateral shoot development in Arabidopsis. We generated OE-GhCUC2m (overexpression GhCUC2m) STTM164 (short tandem target mimic RNA of miR164) lines heterologous expression for gh-miR164, GhCUC2 GhCUC2m Arabidopsis to study controlling branching. overexpression resulted a short-branch phenotype similar...
Cotton (Gossypium spp.) is one of the most important fiber crops worldwide. In last two decades, transgenesis and genome editing have played roles in cotton improvement. However, genotype dependence key bottlenecks generating transgenic gene-edited plants through either particle bombardment or Agrobacterium-mediated transformation. Here, we developed a shoot apical meristem (SAM) cell-mediated transformation system (SAMT) that allowed recalcitrant genotypes including widely grown upland...
Abstract Background Dehydration responsive element-binding ( DREB ) transcription factors are widely present in plants, and involve signalling transduction, plant growth development, stress response. genes have been characterized multiple species. However, only a few studied cotton, one of the most important fibre crops. Herein, genome‑wide identification, phylogeny, expression analysis family performed diploid tetraploid cotton Results In total, 193, 183, 80, 79 putative containing AP2...
Abstract Osteoporotic bone defects, a severe complication of osteoporosis, are distinguished by delayed healing process and poor repair quality. While marrow‐derived mesenchymal stem cells (BMMSCs) the primary origin bone‐forming osteoblasts, their mitochondrial function is impaired, leading to inadequate regeneration in osteoporotic patients. Melatonin well‐known for its antioxidant properties regulation on metabolism. The present study postulated that melatonin has potential enhance...
The repair of critical-sized bone defects poses a significant challenge due to the absence periosteum, which plays crucial role in coordinating processes osteogenesis and vascularization during healing. Herein, we hypothesized that melatonin-encapsuled silk Fibronin electrospun nanofibers (SF@MT) could provide intrinsic induction both angiogenesis, thereby promoting vascularized regeneration. sustained release melatonin from SF@MT resulted favorable biocompatibility superior osteogenic...
Cotton fibers are single cells that develop from the epidermal in outer integument of developing seeds. The processes regulating fiber cell development have been extensively studied; however, spatiotemporal transcriptome and metabolome profiles during early stages remain largely unknown. In this study, we profile dynamics cotton using a combination spatial transcriptomic, single-cell metabolomic analyses. We identify key genes (e.g., DOX2, KCS19.4, BEE3, HOS3.7) metabolites linoleic acid,...
Sucrose non-fermenting-1-related protein kinase 2 (SnRK2) is a plant-specific serine/threonine family involved in the abscisic acid (ABA) signaling pathway and responds to osmotic stress. A genome-wide analysis of this has been conducted previously some plant species, but little known about SnRK2 genes upland cotton (Gossypium hirsutum L.). The recent release G. genome sequence provides an opportunity identify characterize cotton. We identified 20 putative sequences genome, designated as...
Germin-like proteins (GLPs) are water-soluble plant glycoproteins belonging to the cupin superfamily. The important role of GLPs in responses against various abiotic and biotic stresses, especially pathogens, is well validated. However, little known about cotton relation fungal pathogens. Here, a novel GLP gene was isolated from Gossypium hirsutum designated as GhABP19. expression GhABP19 upregulated plants inoculated with Verticillium dahliae Fusarium oxysporum response treatment jasmonic...
Drought and high salinity are two key limiting factors for cotton production. Therefore, more research is focused on the underlying stress response mechanisms of cotton. We first identified cloned a novel gene encoding 525 amino acid in cotton, namely GhWRKY6. Quantitative RT-PCR analysis indicated that GhWRKY6 was induced by NaCl, PEG ABA. Analysis germination rate root length overexpression Arabidopsis resulted plant being hypersensitive to ABA, NaCl treatment. In contrast,...
Verticillium wilt is caused by the soil-borne vascular pathogen dahliae, and affects a wide range of economically important crops, including upland cotton (Gossypium hirsutum). Previous studies showed that expression levels BIN2 were significantly down-regulated during infestation with V. dahliae. However, underlying molecular mechanism in plant regulation against dahliae remains enigmatic. Here, we characterized protein kinase GhBIN2 from Gossypium hirsutum, identified as negative regulator...
Abstract Fungal pathogens secrete effector proteins that regulate host immunity and can suppress basal defence mechanisms against colonization in plants. Verticillium dahliae is a widespread destructive soilborne fungus cause vascular wilt disease reduces plant yields. However, little currently known about how the effectors secreted by V . function. In this study, we analysed identified 34 candidate secretome found Vd424Y , glycoside hydrolase family 11 protein, was highly upregulated during...
The vast majority of the world's cotton varieties produce white fibre, which is then coloured with synthetic dyes during textile processing to fulfil diverse needs consumers. This results in potential environmental pollution and detrimental human animal health. Although there exists naturally cottons, mostly brown green, their poor agronomic performance suboptimal fibre quality have severely hampered widespread applications breeding program. Therefore, an impetus create multicoloured through...
Abstract Background Verticillium wilt is one of the most devasting diseases for many plants, leading to global economic loss. Cotton known be vulnerable its fungal pathogen, dahliae , yet related genetic mechanism remains unknown. Results By genome-wide association studies 419 accessions upland cotton, Gossypium hirsutum we identify ten loci that are associated with resistance against wilt. Among these loci, SHZDI1 / SHZDP2 AYDP1 from chromosome A10 located on a fragment introgressed...
Objective: Aloe-emodin (AE) is an anthraquinone compound extracted from the rhizome of natural plant rhubarb. Initially, it was shown that AE exerts anti-inflammatory effect. Further studies revealed its antitumor activity against various types cancer. However, mechanisms underlying these properties remain unclear. Based on network pharmacology and molecular docking, this study investigated mechanism in treatment hepatocellular carcinoma (HCC), evaluated therapeutic effect through vitro...
Summary Heavy metal pollution poses a significant risk to human health and wreaks havoc on agricultural productivity. Phytoremediation, plant‐based, environmentally benign, cost‐effective method, is employed remove heavy metals from contaminated soil, particularly in or metal‐sensitive lands. However, the phytoremediation capacity of various plant species germplasm resources display genetic diversity, mechanisms underlying these differences remain hitherto obscure. Given its potential...