A5101639900- Colorectal Cancer Treatments and Studies
- Extracellular vesicles in disease
- Cancer Treatment and Pharmacology
- Cancer Genomics and Diagnostics
- T-cell and B-cell Immunology
- Genetic factors in colorectal cancer
- Monoclonal and Polyclonal Antibodies Research
- Wound Healing and Treatments
- Circular RNAs in diseases
- Higher Education and Teaching Methods
- Systemic Lupus Erythematosus Research
- Hepatitis B Virus Studies
- Cancer Diagnosis and Treatment
- Advanced Breast Cancer Therapies
- Renal Diseases and Glomerulopathies
- Gastric Cancer Management and Outcomes
- Mesenchymal stem cell research
- Ferroptosis and cancer prognosis
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- Cerebrovascular and genetic disorders
- Urinary and Genital Oncology Studies
- Bladder and Urothelial Cancer Treatments
- Multiple and Secondary Primary Cancers
- Skin and Cellular Biology Research
Gansu Provincial Hospital
2024
Nanjing General Hospital of Nanjing Military Command
2024
Nanjing University
2024
First Affiliated Hospital of Fujian Medical University
2023-2024
Fujian Medical University
2023-2024
Emory University
2011-2024
Gansu Provincial Maternal and Child Health Hospital
2020-2024
National Clinical Research
2024
Sun Yat-sen University
2022
Sun Yat-sen University Cancer Center
2022
The 2009 pandemic H1N1 influenza demonstrated the global health threat of reassortant strains. Herein, we report a detailed analysis plasmablast and monoclonal antibody responses induced by infection in humans. Unlike antibodies elicited annual vaccinations, most neutralizing were broadly cross-reactive against epitopes hemagglutinin (HA) stalk head domain multiple from cells that had undergone extensive affinity maturation. Based on these observations, postulate plasmablasts producing...
Previous studies have shown that both hepatitis A virus and C inhibit innate immunity by cleaving the mitochondrial antiviral signaling (MAVS) protein, an essential component of virus-activated pathway activates NF-kappaB IFN regulatory factor-3 to induce production type I IFN. For human B (HBV), s-Ag, e-Ag, or HBV virions been suppress TLR-induced activity with reduced IFN-beta subsequent induction IFN-stimulated genes. However, HBV-mediated suppression RIG-I-MDA5 is unknown. In this study,...
Recent studies have begun to reveal critical roles of microRNAs (miRNAs) in the pathogenesis cardiac hypertrophy and dysfunction. In this study, we tested whether a transforming growth factor-β (TGF-β)-regulated miRNA played pivotal role development heart failure (HF). We observed that miR-27b was upregulated hearts cardiomyocyte-specific Smad4 knockout mice, which developed hypertrophy. vitro experiments showed expression could be inhibited by TGF-β1 its overexpression promoted hypertrophic...
MicroRNAs involved in keratinocyte migration and wound healing are largely unknown. Here, we revealed the indispensable role of miR-21 re-epithelialization during mice. In HaCaT cell, could be upregulated by TGF-β1. Similar to effect TGF-β1, overexpression promoted migration. Conversely, knockdown attenuated TGF-β1-induced migration, suggesting that was essential for TGF-β-driven Furthermore, found healing, coincident with temporal expression pattern Consistently, endogenous using a specific...
Abstract Systemic lupus erythematosus (SLE) is an autoimmune disease characterized by multiple autoantibody types, some of which are produced long-lived plasma cells (LLPC). Active SLE generates increased circulating antibody-secreting (ASC). Here, we examine the phenotypic, molecular, structural, and functional features ASC in SLE. Relative to post-vaccination healthy controls, blood from patients with active enriched newly generated mature CD19 − CD138 + ASC, similar bone marrow LLPC....
Hepatocellular carcinoma (HCC) affects males in a significantly higher proportion than females and is one of the human cancers etiologically related to viral factors. Many studies provide strong evidence direct role that hepatitis B virus (HBV) plays hepatic carcinogenesis, but functions HBV surface antigen (HBsAg) X protein (HBx) hepatocarcinogenesis through or indirect mechanisms are still being debated. We generated two gene knock-in transgenic mouse lines by homologous recombination....
9G4(+) IgG Abs expand in systemic lupus erythematosus (SLE) a disease-specific fashion and react with different Ags including B cell apoptotic cells. Their shared use of VH4-34 represents unique system to understand the molecular basis autoreactivity. In this study, large panel recombinant mAbs from single naive memory cells was generated tested against cells, other Ags. Mutagenesis eliminated framework-1 hydrophobic patch (HP) responsible for 9G4 idiotype. The expression HP unselected...
Purpose: Mutations in KRAS are considered to be the main drivers of acquired resistance epidermal growth factor receptor (EGFR) blockade patients with metastatic colorectal cancer (mCRC). However, potential role other genes downstream EGFR signaling pathway conferring has not been extensively investigated.Experimental Design: Using circulating tumor DNA (ctDNA) from mCRC and cetuximab resistance, we developed a targeted amplicon ultra-deep sequencing method screen for low-abundance somatic...
Abstract The dynamics of SARS-CoV-2 RNA structure and their functional relevance are largely unknown. Here we develop a simplified SPLASH assay comprehensively map the in vivo RNA-RNA interactome genome across viral life cycle. We report canonical alternative structures including 5′-UTR 3′-UTR, frameshifting element (FSE) pseudoknot cyclization both cells virions. provide direct evidence interactions between Transcription Regulating Sequences, which facilitate discontinuous transcription. In...
Abstract Anti-dsDNA antibodies are pathogenically heterogeneous, implying distinct origins and antigenic properties. Unexpectedly, during the clinical molecular characterization of autoantibodies to endonuclease DNase1L3 in patients with systemic lupus erythematosus (SLE), we identified a subset neutralizing anti-DNase1L3 previously catalogued as anti-dsDNA. Based on their variable heavy-chain (V H ) gene usage, these can be divided two groups. One group is encoded by inherently autoreactive...
Proline accumulation has been shown to correlate with tolerance drought and salt stresses in plants. We attempt introduce the wild-type, mutant, fusion proBA genes derived from Bacillus subtilis into Arabidopsis thaliana under control of a strong promoter cauliflower mosaic virus 35S (CaMV35S). The transgenic plants produced higher level free proline than overproduction resulted increased osmotic stress Besides, mutation genes, which were proved lead <TEX>$\alpha$</TEX>-glutamyl kinase...
Pancreatic cancer is a multiple genetic disorder with many mutations identified during the progression. Two mouse pancreatic cell lines were established which showed different phenotype in vivo: non-metastatic line, Panc02, and highly metastatic Panc02-H7, derivative of Panc02. In order to investigate whether key genes such as KRAS, TP53 (p53), CDKN2A (p16), SMAD4, ZIP4, PDX-1 contribute phenotypic difference these two cells, we sequenced exonic regions both normal syngeneic pancreas...
Abstract The PI3K/AKT/mTOR signaling pathway is constitutively active in PTEN-deficient cancer cells, and its targeted inhibition has significant anti-tumor effects. However, the efficacy of therapies often limited due to drug resistance. relevant pathways cells treated with PI3K/mTOR inhibitor BEZ235 were screened using a phosphokinase array, further validated following treatment multiple inhibitors or AKT knockdown. correlation between PTEN expression levels STAT3 kinase phosphorylation...
Abstract Leptomeningeal metastasis (LM) is a devastating complication of advanced non–small cell lung cancer (NSCLC). Its diagnosis and monitoring can be challenging. Recently, extracellular vesicle (EV) miRNAs have become new noninvasive diagnostic biomarker. The purpose this study was to examine the clinical value role EV in NSCLC-LM. Next-generation sequencing analysis revealed that with differential expression EVs sera patients NSCLC LM non-LM were detected identify biological markers...
Abstract Adipose-derived mesenchymal stem cells (AD-MSCs) have been shown to ameliorate hyperglycemia in diabetic animals and individuals. However, little is known about whether AD-MSCs affect lipid metabolism. Here we demonstrated for the first time that AD-MSC infusion can significantly suppress increase body weight remarkably improve dyslipidemia db/db obese mice diet-induced obesity mice. Induction of white fat tissue “browning” activation adenosine monophosphate-activated protein kinase...
Normal hepatocytes do not express endogenous uncoupling protein 2 (UCP2) in adult liver, although Kupffer cells do, and it is strikingly induced steatotic liver obese conditions. However, the direct link of UCP2 with pathogenic development diseases injury remains elusive. Here we report that targeted expression to mouse increases susceptibility acute by lipopolysaccharide (LPS) galactosamine (GalN). appears enhance proton leak, leading mild a guanosine diphosphate-repressible manner. Indeed,...
Hepatitis B virus X protein (HBx) participates in the occurrence and development processes of hepatocellular carcinoma (HCC) as a multifunctional regulation factor. However, underlying molecular mechanism remains obscure. Here, we describe use p21HBx/+ mouse SILAM (Stable Isotope Labeling Mammals) strategy to define pathological mechanisms for HBx induced liver cancer. We systematically compared series proteome samples from regular mice, 12- 24-month old mice representing inflammation HCC...
PRSS leads to cetuximab resistance by cleaving the drug, and SPINK1 or modified mAbs can avoid cleavage.
Amyotrophic lateral sclerosis (ALS) is a devastating neurodegenerative disease, pathologically characterized by TDP-43 aggregates. Recent evidence has been indicated that phosphorylated (pTDP-43) present not only in motor neurons but also muscle tissues. However, it unclear whether testing pTDP-43 aggregation tissue would assist the diagnosis of ALS. We propose three key questions: (i) Is detectable routine biopsied muscles? (ii) Can detection discriminate between ALS and non-ALS patients?...
AbstractThe molecular mechanisms underlying hepatitis B virus encoded HBx protein -mediated tumorigenesis are not fully understood. In order to gain a better view of the effects on transcriptional regulation and hepatocarcinogenesis, expression profiles liver tumor tissues from 6 18 month-old p21-HBx transgenic control mice were monitored using oligo microarrays. Data analysis demonstrated that 42 genes de-regulated in both mouse tissues. Gene ontology assisted classified these into...