A5060412844- Cardiac Valve Diseases and Treatments
- Cardiac Structural Anomalies and Repair
- Atrial Fibrillation Management and Outcomes
- Cardiac Fibrosis and Remodeling
- Cardiac and Coronary Surgery Techniques
- Mesenchymal stem cell research
- Cardiovascular Function and Risk Factors
- Cardiac tumors and thrombi
- Aortic Disease and Treatment Approaches
- Autophagy in Disease and Therapy
- Venous Thromboembolism Diagnosis and Management
- Mechanical Circulatory Support Devices
- Inflammatory Biomarkers in Disease Prognosis
- Brucella: diagnosis, epidemiology, treatment
- Heme Oxygenase-1 and Carbon Monoxide
- Advanced Fluorescence Microscopy Techniques
- Connective tissue disorders research
- Ion Transport and Channel Regulation
- Vascular Procedures and Complications
- ATP Synthase and ATPases Research
- Electrospun Nanofibers in Biomedical Applications
- Cardiomyopathy and Myosin Studies
- Burkholderia infections and melioidosis
- S100 Proteins and Annexins
- Optimism, Hope, and Well-being
Peking University People's Hospital
2016-2025
Peking University
2016-2025
Beijing Anzhen Hospital
2021
Capital Medical University
2021
Fu Wai Hospital
2015-2019
Chinese Academy of Medical Sciences & Peking Union Medical College
2014-2019
State Key Laboratory of Cardiovascular Disease
2019
Peking Union Medical College Hospital
2015
Academia Sinica
2015
Huazhong University of Science and Technology
2012
Abstract Cardiac fibrosis in post-myocardial infarction (MI), seen both infarcted and non-infarcted myocardium, is beneficial to the recovery of heart function. But progressively pathological impairs ventricular function leads poor prognosis. FAK has recently received attention as a potential mediator fibrosis, our previous study reported that pharmacological inhibition can attenuate cardiac post MI models. However, long-term effects on adverse remodelling were not clearly investigated. In...
Rationale: While cell-cell interaction plays a critical role in physiology and disease, comprehensive understanding of its dynamics vascular homeostasis diseases is yet absent. Methods: Here, by use single-cell RNA-sequencing multi-color staining, we delineate the cellular composition spatial characterization human aorta with or without aortic dissection (AD). Results: Scrutinization cell subtype alterations revealed significantly changed fibroblast (FB)-smooth muscle (SMC) interactions AD....
Exosomes derived from bone marrow mesenchymal stem cells (BMSCs) and heme oxygenase 1 (HO‑1) attenuate intervertebral disc degeneration (IVDD). However, whether BMSC‑derived exosomes IVDD by delivering HO‑1 to nucleus pulposus (NP) remains be elucidated. Mouse BMSCs were characterized multilineage differentiation surface marker molecule detection. Exo Exo‑HO‑1 isolated HO‑1‑overexpressing ultracentrifugation observing their morphology, detecting the exosome proteins, tumor susceptibility...
Background: To investigate the role of focal adhesion kinase (FAK)-mediated signaling in hypoxia-induced cardiac fibroblasts (CFs) differentiation and fibrosis post-myocardial infarction (MI) on a mice model. Methods: CFs neonatal C57BL/6 were treated under normoxic, hypoxic, or hypoxic+PP2 (known as Src family inhibitor) conditions. Gene expressions FAK, alpha-smooth muscle actin (α-SMA) collagen type I alpha 1 (Col1α1), α-SMA vimentin levels performed by RT-PCR immunofluorescence staining,...
Background : Mesenchymal stem cell (MSC) transplantation is a promising therapy for cardiac repair. However, the efficacy limited by poor viability of MSCs in infarcted heart. Recent findings have implicated that trimetazidine (TMZ) enhanced survival cells under various conditions. as these studies were animal-derived, little information available about effects TMZ on human MSCs. Herein, we propose may protect against apoptosis induced Hypoxia/Serum deprivation (H/SD). Methods: Human...
Background Epidermal growth factor (EGF), latrophilin and seven transmembrane domain-containing protein 1 (ELTD1) is developmentally upregulated in the heart. Little known about relationship between ELTD1 cardiac diseases. Therefore, we aimed to clarify role of pressure overload–induced hypertrophy. Methods Results C57BL/6J wild-type (WT) mice ELTD1-knockout (KO) were subjected left ventricular overload by descending aortic banding (AB). KO exhibited more unfavorable remodeling than WT 28...
Background Mechanical heart valve replacement (MHVR) is an effective method for the treatment of severe disease; however, it subjects patient to lifelong warfarin therapy after MHVR with attendant risk bleeding and thrombosis. Whether internet-based management reduces complications improves quality life remains unknown. Objective This study aimed compare effects conventional approach in patients who received order provide evidence regarding alternative strategies long-term anticoagulation....
Background: We previously reported that lysophosphatidic acid (LPA) promoted cardiomyocyte hypertrophy in vitro via one of its G protein-coupled receptor subtypes, LPA3. In this study, we examined the role LPA3 cardiac induced by isoproterenol (ISO) and myocardial infarction. Methods: vitro, neonatal rat cardiomyocytes (NRCMs) were subjected to knocked-down or pretreated with a β-adrenergic (β-AR) antagonist (propranolol) before LPA/ISO treatment. Cardiomyocyte size hypertrophic gene (ANP,...
Abstract Brucellosis is endemic in the Mediterranean region, South American countries, and Asia. In China, it frequently diagnosed herdsmen who often have contact with livestock. Brucella endocarditis (BE) a rare complication, but leading reason for mortality. We report case of BE bicuspid aortic valve consequent pseudoaneurysm ascending aorta, which has never been reported before. The major educational value lies acknowledging novel presentation happened patient remission appreciation role...
Objective: To evaluate the risk factors and explore mid-term outcomes of postoperative atrial fibrillation (POAF) after minimally invasive direct coronary artery bypass (MIDCAB). Methods: A total 165 patients, who underwent isolated MIDCAB from 2012 to 2015, were enrolled in study retrospectively reviewed. Patients with preoperative arrhythmia, concomitant surgical procedures excluded. All patients continuously monitored for POAF until discharge, two groups formed: non-POAF group (140 71.4%...
Background: Surgeon‘s preference is an important factor in clinical strategy for off pump (OPCAB) or on (ONCAB) coronary artery bypass graft (CABG) surgery. This study analyzed the surgeons’ understanding and propensity both techniques. Methods: A self-reported questionnaire survey was performed. Two sections were included :(1) Q1 questionnaire: to investigate surgeon’s opinion indications of OPCAB ONCAB; (2) Q2 choice ONCAB different situations. Results: The questionnaires sent 169...
The presence of a high left ventricular end-diastolic diameter (LVEDD) has been linked to less favorable outcome in patients undergoing coronary artery bypass grafting (CABG) procedures. However, by taking into consideration the reference size and volume measurements relative patient's body surface area (BSA), it suggested that accuracy predicting outcomes may be improved.We propose BSA weighted LVEDD (bLVEDD) is more accurate predictor CABG compared simply using alone.This study was...
This work aimed to study the beneficial role of human umbilical cord blood-derived mesenchymal stem cellconditioned medium (MSC-CM) in hypoxia-induced apoptosis H9c2 cardiomyoblasts, which serine/heroine kinases (Akt) pathway would be involved. For this, CM was collected by culturing MSCs serum-free DMEM for 24 h, and paracrine factors were analyzed protein chip. cells divided into following groups: control group, hypoxia MSC-CM intervention group (CM group), + Akt phosphorylation inhibitor...
Objective To compare the aortic diameter after isolated valve replacement (AVR) in patients with a bicuspid (BAV) or tricuspid (TAV) and an initially normal ascending aorta. Methods Patients of < 45 mm who had undergone AVR were studied. Ultrasonic cardiographic measurements made pre- postoperatively follow-up data concerning adverse events death analyzed. Results A total 613 included this retrospective study; these, 211 BAV 402 TAV. In both groups, aorta significantly expanded but was...
Background: Surgeon’s preference is an important factor in clinical strategy for off-pump (OPCAB) or on-pump (ONCAB) coronary artery bypass graft (CABG) surgery. This study analyzed surgeons’ understanding of and propensity both techniques. Methods: A survey was performed by self-reported questionnaire. Two sections were included: Q1 questionnaire investigated each surgeon’s opinion on the indications OPCAB ONCAB; Q2 choice ONCAB different situations. Results: The questionnaires sent to 169...
Cardiac hemangioma is relatively rare for primary cardiac tumors. The diagnose mainly based on surgical resection and biopsy, imaging examinations only provide limited diagnostic clues. For those giant hemangiomas, which may raise a risk of rupture, need thoroughly removal. However, meticulous follow-up required due to its possibility recurrence.
<sec> <title>BACKGROUND</title> Mechanical heart valve replacement (MHVR) is an effective method for the treatment of severe disease; however, it subjects patient to lifelong warfarin therapy after MHVR with attendant risk bleeding and thrombosis. Whether internet-based management reduces complications improves quality life remains unknown. </sec> <title>OBJECTIVE</title> This study aimed compare effects conventional approach in patients who received order provide evidence regarding...
<sec> <title>BACKGROUND</title> The presence of a high left ventricular end-diastolic diameter (LVEDD) has been linked to less favorable outcome in patients undergoing coronary artery bypass grafting (CABG) procedures. However, by taking into consideration the reference size and volume measurements relative patient's body surface area (BSA), it suggested that accuracy predicting outcomes may be improved. </sec> <title>OBJECTIVE</title> We propose BSA weighted LVEDD (bLVEDD) is more accurate...
An important challenge in biological research is the development of high-resolution optical methods to label and image cell populations three dimensions deep intact tissue. In present study, we have developed a methodology for whole-organism clearing, building upon previous techniques. SUT (Scheme Update on tissue Transparency); new tissue-clearing agent, can achieve fast effective passive clearing tissues with little protein loss. was created by dissolving 250 g urea, 150 ml TritonX-100 80...
Objective To observate the changing trend of flow in-situ bilateral internal mammary artery as grafts during perioperative period in patients undergoing coronary bypass grafting(CABG), and to compare parameters between LIMA RIMA. Methods A total 57 , 50 males 7 femails, mean aged(57.8±10.1) years, underwent IMA CABG our hospital from August 2016 January 2019. Transthoracic ultrasound examination was performed before CABG. The blood flow, PI(pulsation index) other were measured...
Abstract Objective To explore risk factors and in-hospital complications of new-onset atrial fibrillation (AF) after off-pump coronary artery bypass grafting (OPCAB). Methods In this study 1344 patients who underwent isolated OPCAB from 2012 to 2015, were divided into AF non-AF group according whether postoperative occurred. Results The incidence was 28.57%, mainly appeared within the first 4 days surgery. After binary logistic regression analysis, age, peripheral vascular disease,...