A5013434529- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- Bacterial Genetics and Biotechnology
- DNA Repair Mechanisms
- RNA Research and Splicing
- Genomics and Chromatin Dynamics
- Microbial Metabolic Engineering and Bioproduction
- Bacteriophages and microbial interactions
- CRISPR and Genetic Engineering
- Photosynthetic Processes and Mechanisms
- Peptidase Inhibition and Analysis
- DNA and Nucleic Acid Chemistry
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Sulfur-Based Synthesis Techniques
- Advancements in Battery Materials
- Advanced biosensing and bioanalysis techniques
- Mitochondrial Function and Pathology
- Biofuel production and bioconversion
- ATP Synthase and ATPases Research
- Glycosylation and Glycoproteins Research
- Microtubule and mitosis dynamics
- Chemical Synthesis and Analysis
- Advanced Battery Materials and Technologies
- Antifungal resistance and susceptibility
Center for Life Sciences
2016-2025
Peking University
2017-2025
The Affiliated Yongchuan Hospital of Chongqing Medical University
2024-2025
Chongqing Medical University
2024-2025
Army Medical University
2011-2025
King Center
2025
National Center for Nanoscience and Technology
2021-2024
State Key Laboratory of Oral Diseases
2024
Sichuan University
2024
National Health and Family Planning Commission
2024
Pathogen recognition by nucleotide-binding (NB), leucine-rich repeat (LRR) receptors (NLRs) plays roles in plant immunity. The Xanthomonas campestris pv. effector AvrAC uridylylates the Arabidopsis PBL2 kinase, and latter (PBL2UMP) acts as a ligand to activate NLR ZAR1 precomplexed with RKS1 pseudokinase. Here we report cryo-electron microscopy structures of ZAR1-RKS1 ZAR1-RKS1-PBL2UMP an inactive intermediate state, respectively. ZAR1LRR domain, compared animal NLRLRR domains, is...
Functional nanostructures with high biocompatibility and stability, low toxicity, specificity of targeting to desired organs or cells are great interest in nanobiology medicine. However, the challenge is integrate all these features into a single nanobiostructure, which can be applied biomedical applications eventually clinical settings. In this context, we designed strategy assemble two gold nanoclusters at ferroxidase active sites ferritin heavy chain. Our studies showed that resulting...
DNA replication is initiated at multiple loci to ensure timely duplication of eukaryotic genomes. Sister forks progress bidirectionally, and terminates when two convergent encounter one another. To investigate the coordination forks, we developed a replication-associated in situ HiC method capture chromatin interactions involving nascent DNA. We identify more than 2000 fountain-like structures contacts human mouse genomes, indicative coupling forks. Replication fork interaction not only...
Ovarian cancer is one of the most common cancers among women. Recent studies demonstrated that gene encoding p110α catalytic subunit phosphatidylinositol 3-kinase (PI3K) frequently amplified in ovarian cells. PI3K involved multiple cellular functions, including proliferation, differentiation, antiapoptosis, tumorigenesis, and angiogenesis. In this study, we demonstrate inhibition activity by LY-294002 inhibited cell proliferation induced G 1 cycle arrest. This effect was accompanied...
In the elongation cycle of translation, translocation is process that advances mRNA–tRNA moiety on ribosome, to allow next codon move into decoding center. New results obtained by cryoelectron microscopy, interpreted in light x-ray structures and kinetic data, us develop a model molecular events during translocation.
The Beclin 1 gene is a haplo-insufficient tumor suppressor and plays an essential role in autophagy. However, the molecular mechanism by which functions remains largely unknown. Here we report crystal structure of evolutionarily conserved domain (ECD) at 1.6 Å resolution. ECD exhibits previously unreported fold, with three structural repeats arranged symmetrically around central axis. defines novel class membrane-binding domain, strong preference for lipid membrane enriched cardiolipin. tip...
Hypoxia-inducible factor 1 (HIF-1) is a heterodimeric basic helix-loop-helix transcription composed of HIF-1 alpha and beta/aryl hydrocarbon nuclear translocator subunits. expression induced by hypoxia, growth factors, activation oncogenes. In response to activates the many genes including vascular endothelial (VEGF) erythropoietin. VEGF play an important role in angiogenesis tumor progression. Vanadate widely used industry, potent inducer tumors humans animals. this study, we demonstrate...
Kinetics and cryo-electronmicroscopy data provide insights into GTPase ObgE's role as a ribosome anti-association factor that is modulated by nutrient availability, coupling growth control to biosynthesis protein translation.
To improve chemical cross-linking of proteins coupled with mass spectrometry (CXMS), we developed a lysine-targeted enrichable cross-linker containing biotin tag for affinity purification, cleavage site to separate cross-linked peptides away from after enrichment, and spacer arm that can be labeled stable isotopes quantitation. By locating the flexible on surface 70S ribosome, show this trifunctional is effective at attaining structural information not easily attainable by crystallography...