A5103043185- SARS-CoV-2 and COVID-19 Research
- HIV Research and Treatment
- Animal Virus Infections Studies
- HIV/AIDS drug development and treatment
- COVID-19 Clinical Research Studies
- Cervical Cancer and HPV Research
- SARS-CoV-2 detection and testing
- Hepatitis B Virus Studies
- Virus-based gene therapy research
- Viral gastroenteritis research and epidemiology
- HIV/AIDS Research and Interventions
- Viral Infections and Outbreaks Research
- Hepatitis C virus research
- Immunotherapy and Immune Responses
- vaccines and immunoinformatics approaches
- Immune Cell Function and Interaction
- Hepatitis Viruses Studies and Epidemiology
- Molecular Biology Techniques and Applications
- Bacteriophages and microbial interactions
- Respiratory viral infections research
- Monoclonal and Polyclonal Antibodies Research
- Herpesvirus Infections and Treatments
- Viral Infections and Vectors
- Biosensors and Analytical Detection
- Cytomegalovirus and herpesvirus research
National Institutes for Food and Drug Control
2016-2025
China Electronics Standardization Institute
2025
Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital
2023
Nanjing University of Posts and Telecommunications
2022
Chinese Academy of Sciences
2021
Czech Academy of Sciences, Institute of Microbiology
2021
National Institute for Viral Disease Control and Prevention
2021
Beijing Center for Disease Prevention and Control
2021
Center for Disease Control
2021
National Medical Products Administration
2021
Pseudoviruses are useful virological tools because of their safety and versatility, especially for emerging re-emerging viruses. Due to its high pathogenicity infectivity the lack effective vaccines therapeutics, live SARS-CoV-2 has be handled under biosafety level 3 conditions, which hindered development therapeutics. Based on a VSV pseudovirus production system, pseudovirus-based neutralization assay been developed evaluating neutralizing antibodies against in 2 facilities. The key...
A steric block to SARS-CoV-2 In response infection by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the immune system makes antibodies, many of which target spike protein, a key player in host cell entry. Antibodies that potently neutralize virus hold promise as therapeutics and could inform vaccine design. Lv et al. report humanized monoclonal antibody protected against mouse model. The cryo–electron microscopy structure, together with biochemical, cellular, virological...
The 501Y.V2 variants of SARS-CoV-2 containing multiple mutations in spike are now dominant South Africa and rapidly spreading to other countries. Here, experiments with 18 pseudotyped viruses showed that the do not confer increased infectivity cell types except for murine ACE2-overexpressing cells, where a substantial increase was observed. Notably, susceptibility 12 17 neutralizing monoclonal antibodies substantially diminished, neutralization ability sera from convalescent patients...
Glycans on the human immunodeficiency virus (HIV) envelope glycoprotein (Env) play an important role in viral infection and evasion of neutralization by antibodies. In this study, all 25 potential N-linked glycosylation sites (PNGS) HIV-1 CRF07_BC Env, FE, were mutated individually to study effect their removal infectivity, virion production, antibody-mediated neutralization. Removal specific N-glycosylation has a significant infectivity phenotype. Six these mutants located V1/V2 C1/C2...
Emerging mutations in SARS-CoV-2 cause several waves of COVID-19 pandemic. Here we investigate the infectivity and antigenicity ten emerging variants-B.1.1.298, B.1.1.7(Alpha), B.1.351(Beta), P.1(Gamma), P.2(Zeta), B.1.429(Epsilon), B.1.525(Eta), B.1.526-1(Iota), B.1.526-2(Iota), B.1.1.318-and seven corresponding single amino acid receptor-binding domain using pseudovirus. The results indicate that pseudovirus most variants (except B.1.1.298) display slightly increased human monkey cell...
From December 2022 to January 2023, SARS-CoV-2 infections caused by BA.5 and BF.7 subvariants of B.1.1.529 (Omicron) spread in China. It is urgently needed evaluate the protective immune responses infected individuals against current circulating variants predict future potential infection waves, such as BQ.1.1, XBB.1.5, CH1.1 variants. In this study, we constructed a panel pseudotyped viruses for past variants, including D614G, Delta, BA.1, BA.5, BF.7, XBB.1.5 CH.1.1. We investigated...
Abstract Pseudoviruses are useful virological tools because of their safety and versatility; however the low titer these viruses substantially limits wider applications. We developed a highly efficient pseudovirus production system capable yielding 100 times more rabies than traditional method. Employing high-titer pseudoviruses, we have robust in vitro vivo neutralization assays for evaluation vaccine, which traditionally relies on live-virus based assays. Compared with current rapid...
Severe acute respiratory syndrome coronavirus 2 variants have continued to emerge in diverse geographic locations with a temporal distribution. The Lambda variant containing multiple mutations the spike protein, has thus far appeared mainly South America. harbours two receptor binding domain, L452Q and F490S, which may change its infectivity antigenicity neutralizing antibodies. In this study, we constructed 10 pseudoviruses study each individual amino acid mutation's effect on viral...
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants, particularly those with multiple mutations in receptor-binding domain (RBD), pose a critical challenge to the efficacy of disease 2019 (COVID-19) vaccines and therapeutic neutralizing monoclonal antibodies (mAbs). Omicron sublineages BA.1, BA.2, BA.3, as well recent emergence C.1.2, B.1.630, B.1.640.1, B.1.640.2, have RBD may lead antibody evasion. It is urgent evaluate antigenic change above seven variants against...
Marburg virus (MARV) can cause lethal hemorrhagic fever in humans. Handling of MARV is restricted to high-containment biosafety level 4 (BSL-4) facilities, which greatly impedes research into this virus. In study, a high titer pseudovirus was generated through optimization the HIV backbone vectors, ratio vector glycoprotein expression vector, and transfection reagents. An vitro neutralization assay an vivo bioluminescent imaging mouse model for were developed based on pseudovirus. Protective...
The ubiquitously-expressed proteolytic enzyme furin is closely related to the pathogenesis of SARS-CoV-2 and therefore represents a key target for antiviral therapy. Based on bioinformatic analysis pseudovirus tests, we discovered second functional site located in spike protein. Furin still increased infectivity mutated 293T-ACE2 cells when canonical polybasic cleavage (682–686) was deleted. However, K814A mutation eliminated enhancing effect virus infection. inhibitor prevented infection by...
Abstract The origin and intermediate host for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is yet to be determined. Coronaviruses found closely related SARS-CoV-2 include RaTG13 derived from bat two clusters (PCoV-GD PCoV-GX) of coronaviruses identified in pangolin. Here, we studied the infectivity antigenicity patterns three coronaviruses. Compared with other viruses, showed almost no a variety cell lines. pangolin similar infectious activity. However, SARS-CoV-2-susceptible...
Several human papillomavirus (HPV) L1-based virus-like particle (VLP) vaccines are in development to meet future global vaccination needs. Type-specific monoclonal antibodies with good reactivity all types of urgently needed evaluate vaccine potency. In this study, binding activity, neutralizing conformational sensitivity, immunodominance serum, and versatility were compared among antibodies. A broad-spectrum antibody (C4-F5-127) was selected as the capture antibody; four type-specific...
Low antigen immunogenicity poses a significant challenge in vaccine development, often leading to inadequate immune responses and reduced efficacy. Therefore, the discovery of potent immune-enhancing adjuvants is crucial. STING (stimulator interferon genes) agonists are promising class which have been identified various cells activated response DNA fragments, triggering broad range type-I interferon-dependent responses. Integrating with components an ideal strategy bolster vaccine-induced...
Pseudovirion-based neutralization assay is considered the gold standard method for evaluating immune response to human papillomavirus (HPV) vaccines. In this study, we developed a multicolor simultaneously detect neutralizing antibodies against different HPV types. FluoroSpot was used interpret fluorescent protein expression instead of flow cytometry. The results and cytometry showed good consistency, with R² > 0.98 log-transformed IC50 values. Regardless reporter color, single-, dual-,...
Since the emergence of influenza A(H7N9) virus in 2013, there have been 5 waves epidemics China. However, evolution hemagglutinin (HA) protein antigenicity has not systematically investigated.To better understand how antigenic drift HA proteins (A)H7N9 occurs, 902 sequences from a public database were retrieved and analyzed. Fifty-three mutants with single amino acid substitutions introduced into pseudoviruses, their characteristics analyzed using pseudovirus-based assays.The frequencies 9...
Because of its high infectivity in humans and the lack effective vaccines, Nipah virus is classified as a category C agent handling has to be performed under biosafety level 4 conditions non-endemic countries, which hindered development vaccines. Based on highly efficient pseudovirus production system using modified HIV backbone vector, pseudovirus-based mouse model been developed for evaluating efficacy vaccines 2 facilities. For first time, correlates protection have identified model. The...