A5102791418- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- Long-Term Effects of COVID-19
- Microbial Natural Products and Biosynthesis
- SARS-CoV-2 detection and testing
- Enzyme Catalysis and Immobilization
- Pharmacogenetics and Drug Metabolism
- COVID-19 epidemiological studies
- Microbial Metabolic Engineering and Bioproduction
- Respiratory viral infections research
- Influenza Virus Research Studies
- Fungal Biology and Applications
- Biochemical and Molecular Research
- Monoclonal and Polyclonal Antibodies Research
- Retinal and Optic Conditions
- Virus-based gene therapy research
- Dermatology and Skin Diseases
- interferon and immune responses
- Plant biochemistry and biosynthesis
- Viral Infections and Immunology Research
- Pharmacological Effects of Natural Compounds
- Cardiac tumors and thrombi
- Renal Diseases and Glomerulopathies
- Immune Cell Function and Interaction
- Asthma and respiratory diseases
Institute of Laboratory Animal Science
2019-2025
Chinese Academy of Medical Sciences & Peking Union Medical College
2019-2025
First Affiliated Hospital of Zhengzhou University
2025
Xi'an Jiaotong University
2024
Model Animal Research Center
2022-2024
Qingdao Institute of Bioenergy and Bioprocess Technology
2016-2023
Chinese Academy of Sciences
2016-2023
University of Chinese Academy of Sciences
2018-2022
Qinghai New Energy (China)
2021-2022
National Center for Nanoscience and Technology
2018
Abstract The recent outbreak of coronavirus disease (COVID-19) caused by SARS-CoV-2 infection in Wuhan, China has posed a serious threat to global public health. To develop specific anti-coronavirus therapeutics and prophylactics, the molecular mechanism that underlies viral must first be defined. Therefore, we herein established spike (S) protein-mediated cell–cell fusion assay found showed superior plasma membrane capacity compared SARS-CoV. We solved X-ray crystal structure six-helical...
Coronavirus disease 2019 (COVID-19), which is caused by infection with the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has become a global pandemic. It unclear whether convalescing patients have risk of reinfection. We generated rhesus macaque model SARS-CoV-2 that was characterized interstitial pneumonia and systemic viral dissemination mainly in gastrointestinal tracts. Rhesus macaques reinfected identical strain during early recovery phase initial did not show detectable...
Abstract A global pandemic of Corona Virus Disease 2019 (COVID-19) caused by severe acute respiratory syndrome CoV-2 (SARS-CoV-2) is ongoing spread. It remains unclear whether the convalescing patients have a risk reinfection. Rhesus macaques were rechallenged with SARS-CoV-2 during an early recovery phase from initial infection characterized weight loss, interstitial pneumonia and systemic viral dissemination mainly in gastrointestinal tracts. The monkeys identical strain failed to produce...
Abstract Background Since December 2019, an outbreak of the Corona Virus disease 2019 (COVID‐19) caused by severe acute respiratory syndrome coronavirus (SARS‐CoV‐2) in Wuhan, China, has become a public health emergency international concern. The high fatality aged cases SARS‐CoV‐2 was need to explore possible age‐related phenomena with non‐human primate models. Methods Three 3‐5 years old and two 15 rhesus macaques were intratracheally infected SARS‐CoV‐2, then analyzed clinical signs,...
Abstract Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is highly transmitted through the route, but potential extra-respiratory routes of SARS-CoV-2 transmission remain uncertain. Here we inoculated five rhesus macaques with 1 × 10 6 TCID 50 conjunctivally (CJ), intratracheally (IT), and intragastrically (IG). Nasal throat swabs collected from CJ IT had detectable viral RNA at 1–7 days post-inoculation (dpi). Viral was detected in anal only group dpi. undetectable tested...
The coronavirus disease 2019 (COVID-19) pandemic is an ongoing global health concern, and effective antiviral reagents are urgently needed. Traditional Chinese medicine theory-driven natural drug research development (TCMT-NDRD) a feasible method to address this issue as the traditional formulae have been shown in treatment of COVID-19. Huashi Baidu decoction (Q-14) clinically approved formula for COVID-19 therapy with anti-inflammatory effects. Here, integrative pharmacological strategy was...
Abstract The outbreak of Corona Virus Disease 2019 caused by the severe acute respiratory syndrome coronavirus (SARS-CoV-2) is highly transmitted. potential extra-respiratory transmission routes remain uncertain. Five rhesus macaques were inoculated with 1×10 6 TCID 50 SARS-CoV-2 via conjunctival (CJ), intratracheal (IT), and intragastric (IG) routes, respectively. Remarkably, CJ inoculated-macaques developed mild interstitial pneumonia viral load was detectable in swabs at 1 days...
Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) has spread across the world and impacted global healthcare systems. For clinical patients, COVID-19 not only induces pulmonary lesions but also affects extrapulmonary organs. An ideal animal model that mimics in humans terms of induced systematic is urgently needed. Here, we report Syrian hamster highly permissive to SARS-CoV-2 exhibit diffuse alveolar damage multi-organs damage, including spleen, lymph nodes, different segments...
Abstract Influenza A virus may circulate simultaneously with the SARS-CoV-2 virus, leading to more serious respiratory diseases during this winter. However, influence of these viruses on disease outcome when both influenza and are present in host remains unclear. Using a mammalian model, sequential infection was performed ferrets K18- hACE2 mice, following H1N1. We found that co-infection H1N1 extended duration clinical manifestation COVID-19, enhanced pulmonary damage, but reduced viral...
Abstract Angiopoietin‐2 (Angpt2) plays a critical role in angiogenesis and tumour progression. Therapeutic targeting of Angpt2 has been implicated as promising strategy for cancer treatment. Whereas miRNAs are emerging important modulators angiogenesis, regulation by not established. Here we firstly report that Ang2 is targeted microRNA , miRNA ‐542‐3p, which inhibits progression impairing Ang2's pro‐angiogenic activity. In cultured endothelial cells, miR ‐542‐3p inhibited translation mRNA...
Abstract Recent advances in cancer immunotherapy have exploited the efficient potential of natural killer (NK) cells to kill tumor through antibody‐dependent cell‐mediated cytotoxicity (ADCC). However, this therapeutic strategy is seriously limited by antigen heterogeneity since antibodies can only recognize specific antigens. In work, modified or their Fc fragments that target solid tumors without necessity presentation on are developed. Briefly, monoclonal conjugated with N‐terminus pH low...
We simulated 3 transmission modes, including close-contact, respiratory droplets and aerosol routes, in the laboratory. Severe acute syndrome coronavirus 2 (SARS-CoV-2) can be highly transmitted among naive human angiotensin-converting enzyme (hACE2) mice via close contact because 7 of 13 hACE2 were SARS-CoV-2 antibody seropositive 14 days after being introduced into same cage with infected-hACE2 mice. For droplets, antibodies from 10 showed seropositivity introduction mice, separated by...
Significance Here, we elucidate the full biosynthetic pathway of fungal natural product mycophenolic acid (MPA). Besides intriguing enzymatic mechanisms, reveal that MPA enzymes are elegantly compartmentalized; oxygenase MpaB′ is long-sought enzyme responsible for initiating oxidative cleavage farnesyl side chain; and subcellular localization acyl-coenzyme A hydrolase MpaH′ in peroxisomes required unique cooperation between β-oxidation catabolism machineries. This work highlights importance...
Domestic cats, an important companion animal, can be infected with severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). This has aroused concern regarding the ability of domestic cats to spread virus that causes coronavirus disease 2019. We systematically demonstrated pathogenesis and transmissibility SARS-CoV-2 in cats. Serial passaging between dramatically attenuated viral transmissibility, likely owing variations amino acids receptor-binding domain sites angiotensin-converting...
It is well accepted that vaccination by oral administration has many advantages over injected parenteral immunization. The present study focuses on whether with a DNA vaccine could induce protective immunity against respiratory challenge infection. M1 gene of influenza A virus was used to construct using pcDNA 3.1(+) plasmid, eukaryotic expression vector. cationic liposomes were deliver the constructed vaccine. In vitro and in vivo observed cell line intestine orally vaccinated C57BL/6 mice,...
Abstract Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is transmitted on mink farms between minks and humans in many countries. However, the systemic pathological features of SARS-CoV-2-infected are mostly unknown. Here, we demonstrated that were largely permissive to SARS-CoV-2, characterized by severe diffuse alveolar damage, lasted at least 14 days post inoculation (dpi). We first reported infected displayed multiple organ-system lesions accompanied an increased...
As an edible and medicinal fungus, Dictyophora indusiata is well-known for its morphological elegance, distinctive taste, high nutritional value, therapeutic properties. In this study, eighteen compounds (1–18) were isolated identified from the ethanolic extract of D. indusiata; four (1–4) previously undescribed. Their molecular structures absolute configurations determined via a comprehensive analysis spectroscopic data (1D/2D NMR, HRESIMS, ECD, XRD). Seven examined their anti-inflammatory...
Enterovirus 71 (EV71) is a common pathogen responsible for hand, foot, and mouth disease (HFMD), leading to severe neurological complications even death. However, the mechanisms underlying EV71-induced remain unclear, no effective specific treatments are available. In this study, we successfully infected mice of different ages using mouse-adapted EV71 strain, resulting in mortality. We compared immune system responses between uninfected identify key pathogenic targets during infection. Our...