A5080587298- Influenza Virus Research Studies
- Viral gastroenteritis research and epidemiology
- Computational Drug Discovery Methods
- SARS-CoV-2 and COVID-19 Research
- Pharmacological Effects of Natural Compounds
- Respiratory viral infections research
- Bone Tissue Engineering Materials
- Animal Virus Infections Studies
- Immune Response and Inflammation
- Synthesis and Characterization of Heterocyclic Compounds
- Graphene and Nanomaterials Applications
- Nanocomposite Films for Food Packaging
- Click Chemistry and Applications
- Organometallic Compounds Synthesis and Characterization
- Cytokine Signaling Pathways and Interactions
- Nanoparticle-Based Drug Delivery
- Orthopedic Surgery and Rehabilitation
- Space Satellite Systems and Control
- Bone fractures and treatments
- Antimicrobial agents and applications
- Andrographolide Research and Applications
- Advanced Chemical Sensor Technologies
- Tendon Structure and Treatment
- Metal-Organic Frameworks: Synthesis and Applications
- Synthesis and Biological Evaluation
Institute of Microbiology
2013-2024
Chinese Academy of Sciences
2013-2024
Czech Academy of Sciences, Institute of Microbiology
2023-2024
Huazhong University of Science and Technology
2024
Beijing Chao-Yang Hospital
2020-2023
Capital Medical University
2020-2023
Shaoxing People's Hospital
2022-2023
Second Affiliated Hospital of Zhejiang University
2021
Jiangxi Normal University
2016
University of Chinese Academy of Sciences
2013-2016
Abstract COVID-19 was declared a pandemic on March 11 by WHO, due to its great threat global public health. The coronavirus main protease (M pro , also called 3CLpro) is essential for processing and maturation of the viral polyprotein, therefore recognized as an attractive drug target. Here we show that clinically approved anti-HCV drug, Boceprevir, pre-clinical inhibitor against feline infectious peritonitis (corona) virus (FIPV), GC376, both efficaciously inhibit SARS-CoV-2 in Vero cells...
In plants, transcriptional regulation is the most important tool for modulating flavonoid biosynthesis. The AtMYB12 gene from Arabidopsis thaliana has been shown to regulate expression of key enzyme genes involved in biosynthesis, leading increased accumulation flavonoids. this study, codon-optimized was chemically synthesized. Subcellular localization analysis onion epidermal cells indicated that localized nucleus. Its overexpression significantly flavonoids and enhanced salt drought...
The coronavirus disease 2019 (COVID-19) pandemic is an ongoing global health concern, and effective antiviral reagents are urgently needed. Traditional Chinese medicine theory-driven natural drug research development (TCMT-NDRD) a feasible method to address this issue as the traditional formulae have been shown in treatment of COVID-19. Huashi Baidu decoction (Q-14) clinically approved formula for COVID-19 therapy with anti-inflammatory effects. Here, integrative pharmacological strategy was...
Abstract We have surveyed avian influenza virus (AIV) genomes from live poultry markets within China since 2014. Here we present a total of 16,091 samples that were collected May 2016 to February 2019 in 23 provinces and municipalities China. identify 2048 AIV-positive perform next generation sequencing. rates (12.73%) had decreased substantially 2016, compared during 2014–2016 (26.90%). Additionally, H9N2 has replaced H5N6 H7N9 as the dominant AIV subtype both chickens ducks. Notably, novel...
The outbreak of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has developed into an unprecedented global pandemic. Nucleoside analogs, such as Remdesivir and Favipiravir, can serve the first-line broad-spectrum antiviral drugs by targeting viral polymerases. However, underlying mechanisms for efficacies these are far from well understood. Here, we reveal that a pyrazine derivative, could be incorporated RNA products mimicking both adenine guanine nucleotides. This drug thus...
SARS-CoV (the SARS-Associated Coronavirus) was reported as a novel virus member in the coronavirus family, which cause of severe acute respiratory syndrome. Coronavirus replication occurs through unique mechanism employing Leader sequence transcripts when initiating transcription from genome. Therefore, we cloned SARS-CoV(BJ01), is identical to that identified SARS-CoV(HKU-39849), and constructed specific siRNA targeting sequence. Using EGFP RFP reporter genes fused with sequence,...
A TCM formula has played an important role in the treatment of COVID-19 China. However, mechanism action is still unclear.
Tendons are fibroblastic structures that link muscle and bone. There two kinds of tendon injuries, including acute chronic. Each form injury or deterioration can result in significant pain loss function. The recovery damage is a complex time-consuming process. Depending on the anatomical location tissue, clinical outcomes not same. healing wound process divided into three stages overlap: inflammation, proliferation, tissue remodeling. Furthermore, curing has high re-tear rate. Faced with...
Since 2013, H7N9 avian influenza viruses (AIVs) have caused more than 1,600 human infections, posing a threat to public health. An emerging concern is whether AIVs will cause pandemics among humans. Molecular analysis of hemagglutinin (HA), which critical determinant interspecies transmission, shows that the current are still dual-receptor tropic, indicating limited human-to-human transmission potency. Mutagenesis and structural studies reveal G186V substitution sufficient for acquire...
Abstract The outbreak of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has developed into an unprecedented global pandemic. Nucleoside analogues, such as Remdesivir and Favipiravir, can serve the first-line broad-spectrum antiviral drugs against newly emerging viral diseases. Recent clinical trials these two for SARS-CoV-2 treatment revealed efficacies well side effects with different extents 1–4 . As a pyrazine derivative, Favipiravir could be incorporated RNA products by...
ABSTRACT The neuraminidase stalk of the newly emerged H7N9 influenza virus possesses a 5-amino-acid deletion. This study focuses on characterizing biological functions with varied lengths. Results indicate that deletion had no impact infectivity or replication in vitro vivo compared to full-length stalk, but enhanced virulence mice was observed for encoding 19- 20-amino-acid deletion, suggesting N9 length impacts mammals, as N1 does.
Zanamivir and oseltamivir are principal influenza antiviral drugs that target viral neuraminidase (NA), but resistant viruses containing mutant NAs with diminished drug affinity increasingly emerging. Using the structural knowledge of both drug-binding sites their spatial arrangement on homotetrameric NA, we have developed a tetravalent zanamivir (TZ) molecule exhibited marked increases in NA binding affinity, inhibition enzyme activity, vitro plus vivo efficacy over zanamivir. TZ functioned...
The coronavirus disease 2019 (COVID-19) pandemic, caused by severe acute respiratory syndrome 2 (SARS-CoV-2), has led to more than 5 million deaths worldwide date. Due the limited therapeutic options so far available, target-based virtual screening with LC/MS support was applied identify novel and high-content compounds 1–4 inhibitory effects on SARS-CoV-2 in Vero E6 cells from plant Dryopteris wallichiana. These were also evaluated against Calu-3 showed unambiguous activity. inhibition...
Influenza virus neuraminidase (NA) drug resistance is one of the challenges to preparedness against epidemic and pandemic influenza infections. NA N1- N2-containing viruses are primary cause seasonal epidemics past pandemics. The structural functional basis underlying N1 well characterized. Yet N2 strain not understood. Here, we confirm that replacement E119 or I222 results in multidrug resistance, when replacements occur together, sensitivity inhibitors (NAI) reduced severely. Using...
Inflammatory microenvironment is significant to the differentiation and function of mesenchymal stem cells (MSCs). It evidentially influences osteoblastogenesis MSCs. IL-34, a newly discovered cytokine, playing key role in metabolism. However, research on its functional osteogenesis MSCs was rarely reported. Here, we described regulatory effects low-dose IL-34 both osteoclastogenesis.We performed osteogenic hBMSCs by exogenous overexpressed vitro, so were osteoclastogenesis mBMMs...
During an investigation in October 2018, two people with diarrhoea, mild abdominal pain, and arthralgia symptoms Guangxi, China, were identified as infected by H9N2 avian influenza virus (AIV). Four AIVs isolated from one of patients, a pet cat, dead chicken (two respective isolates its lung kidney tissues) bred the patients at backyard farm. Epidemiological indicated that newly bought died first, clinical syndromes appeared subsequently owners cat. Furthermore, individuals possessed high...
Abstract Polyvinyl alcohol (PVA) hydrogels have potential applications in bionic articular cartilage due to their good biocompatibility and low friction. However, the lack of adequate mechanical properties is a key obstacle for PVA replace natural cartilage. In this study, polyethylene glycol (PEG) was introduced into hydrogel, (PVA/PEG) composite gels were prepared by blending physical cross‐linking method. The PVA/PEG treated with simple novel organic solvent immersion dehydration improve...
Drugs that cure COVID-19 have been marketed; however, this disease continues to ravage the world without becoming extinct, and thus, drug discoveries are still relevant. Since M
Branched or encapsulating nanostructures with controlled architectures are produced using flow fluctuation in a vapor–liquid–solid process. The induces catalyst coalescence division (see figure). technique is illustrated by the synthesis of iron-encapsulating carbon nanotubes position, allowing modulation magnetic properties.