A5003565023- Bone Metabolism and Diseases
- Mesenchymal stem cell research
- Graphene and Nanomaterials Applications
- Tendon Structure and Treatment
- Bone Tissue Engineering Materials
- Bone fractures and treatments
- Bone health and treatments
- Phagocytosis and Immune Regulation
- Heat shock proteins research
- Total Knee Arthroplasty Outcomes
- Orthopedic Infections and Treatments
- Immune Response and Inflammation
- Elbow and Forearm Trauma Treatment
- Growth Hormone and Insulin-like Growth Factors
- Cytokine Signaling Pathways and Interactions
- Orthopedic Surgery and Rehabilitation
- RNA Research and Splicing
- Bone and Joint Diseases
- Cancer Research and Treatments
- Cancer Cells and Metastasis
- S100 Proteins and Annexins
- TGF-β signaling in diseases
- Foot and Ankle Surgery
- Cancer-related molecular mechanisms research
- Diabetic Foot Ulcer Assessment and Management
Second Affiliated Hospital of Zhejiang University
2015-2025
Zhejiang University
2015-2024
Life Science Institute
2022
Harvard University
2020
Zhejiang University Medical College Affiliated Stomatological Hospital
2018-2019
Hunan Agricultural University
2008
The imbalance of bone homeostasis is the root cause osteoporosis. However current therapeutic approaches mainly focus on either anabolic or catabolic pathways, which often fail to turn imbalanced metabolism around. Herein we reported that a SIRT-1 agonist mediated molecular strategy reverse in by simultaneously regulating osteogenesis and osteoclastogenesis via locally sustained release SRT2104 from mineral coated acellular matrix microparticles. Immobilization coating (MAM/SRT) harnessing...
The osteo-immunomodulatory properties of biomaterials play an important role in the outcomes bone regeneration. Graphene oxide (GO) has been widely applied many research fields due to its unique properties. However, immunomodulatory GO as a biomaterial for tissue engineering are still unclear.In this study, we evaluated Inflammatory response RAW264.7 cells influenced by GO. Then osteogenic differentiation BMSCs, and angiogenic human umbilical vein endothelial (HUVECs) stimulation with GO/RAW...
Insulin-like growth factor-binding protein 7 (IGFBP7), a low-affinity IGF binder, may play an important role in bone metabolism. However, its function osteogenic differentiation of human marrow-derived mesenchymal stem cells (BMSCs) remains unclear. Therefore, we investigated effects on differentiation. Overexpression IGFBP7 enhanced the expression osteo-specific genes and proteins, knockdown decreased osteogenesis-specific markers. More mineral deposits higher alkaline phosphatase activity...
Microenvironmental conditions can influence the differentiation and functional roles of mesenchymal stem cells (MSCs). Recent studies have suggested that an inflammatory microenvironment significantly affect osteogenic MSCs. Here, we show, for first time, IL-10 has concentration-dependent, dual in osteogenesis human bone marrow (hBMSCs). Low physiologic concentrations (0.01–1.0 ng/ml) activate p38/MAPK signaling pathway to promote hBMSCs, but higher pathologic doses (10–100 inhibit by...
Heat shock proteins have protective effects when cells are exposed to stress. However, the relationship between extracellular heat protein 70 (eHSP70) and osteogenesis of hMSCs has not been reported. The results this study showed that HSP70 (200 ng/ml) increases alkaline phosphatase activity promotes hMSC mineralization. Under osteogenic induction conditions, significantly upregulated expression osteo‐specific genes, such as runt family transcription factor Runx2 osterix (OSX). Comparative...
Abstract HSPA1A , which encodes cognate heat shock protein 70, plays important roles in various cellular metabolic pathways. To investigate its effects on osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs), expression level was compared between undifferentiated and differentiated BMSCs. Rat overexpression BMSCs increased osteoblast-specific gene expression, alkaline phosphatase activity mineral deposition vitro . Moreover, it upregulated β-catenin downregulated DKK1...
Management of fracture healing with a large bone defect remains tricky subject in orthopedic trauma. Enhancing osteogenesis human marrow-derived mesenchymal stem cells (hBMSCs) is one the useful therapeutic strategies for healing. Previous studies have revealed that Apelin may play an important role metabolism. However, its function hBMSCs unclear. Therefore, this study, we investigated effects and mechanism on osteogenic differentiation.We by both exogenous protein overexpression vitro....
High mobility group box 1 (HMGB1) protein has been previously detected in the inflammatory microenvironment of bone fractures. It is well known that HMGB1 acts as a chemoattractant to mesenchymal stem cells (MSCs). In present study, effects on cytokine secretion from MSCs were determined, and molecular mechanisms underlying these osteogenic differentiation elucidated. To detect secretion, antibody array assays performed, which demonstrated HGMB1 induced differential cytokines are...
Abstract Adipose‐derived stem cells (ADSCs)‐based tissue engineering was a promising method to treat intervertebral disc degeneration. Type II collagen is native component in the nucleus pulposus (NP), and has ability promote ADSCs differentiate into NP‐like cells. In this article, we aimed establish genipin‐cross‐linked three‐dimensional (3D) type scaffold, determine biological effects of scaffold on differentiating phenotype. Different concentrations genipin were used cross‐link 3D...
High mobility group box 1 (HMGB1) epxression has been found in the inflammatory microenvironment of fractures. It is well known that HMGB1 acts as a chemoattractant for mesenchymal stem cells (MSCs); however, effects on MSC migration and osteoblastic differentiation, signaling pathways involved these effects, have not yet elucidated. In this study, we aimed to investigate mechanisms involved, using vitro models. We HMGB1, varying concentrations, promoted differentiation MSCs, synthesis...
Abstract Forkhead box protein A2 (FOXA2) is a core transcription factor that controls cell differentiation and may have an important role in bone metabolism. However, the of FOXA2 during osteogenic marrow-derived mesenchymal stem cells (BMSCs) remains largely unknown. In this study, decreased expression was observed rat BMSCs (rBMSCs). knockdown significantly increased osteoblast-specific gene expression, number mineral deposits alkaline phosphatase activity, whereas overexpression inhibited...
Hesperetin has been suggested to be involved in bone strength. We aimed investigate the effects of hesperetin on osteogenic differentiation human mesenchymal stem cells and its related mechanisms. showed that promoted vitro. It potentially exerts via ERK Smad signaling pathways. Using a rat osteotomy model, we combined with hesperetin/gelatin sponge scaffold resulted accelerated fracture healing vivo. Due low cost hesperetin, it could used as growth factor for tissue engineering or surgical...
Abstract Objectives Insulin‐like growth factor‐binding protein 7 (IGFBP7) is a low‐affinity insulin factor (IGF) binder that may play an important role in bone metabolism. We previously reported IGFBP7 enhanced osteogenic differentiation of marrow‐derived mesenchymal stem cells (BMSCs) via the Wnt/β‐catenin signalling pathway. In this study, we tried to reveal its function osteoclast and osteoporosis. Methods used both vitro vivo studies investigate effects on RANKL‐induced...
Increasing evidence indicates that the osteogenic differentiation of mesenchymal stem cells (MSCs) is related to bone formation, heterotopic ossification, and even vascular calcification. Therefore, it essential understand microenvironment regulates these processes. The Klotho gene plays an important role in tissue mineralization, its secreted protein functions as a hormone. We investigated effects on osteogenesis human marrow MSC (hBMSCs). To this end, received medium with or without...
Fracture nonunion and delayed union continue to pose challenges for orthopedic surgeons. In the present study, we combined HMGB1 gelatin sponges with MSC sheets promote bone healing after surgical treatment of rat tibial fractures. The sponge scaffolds supported expansion mesenchymal stem cells (MSCs) promoted osteogenic differentiation MSCs sheets. Lentiviral vectors were then used overexpress in MSCs. results indicated that promotes through STAT3 pathway. Both siRNA a inhibitor...