A5012946544- Spine and Intervertebral Disc Pathology
- Musculoskeletal pain and rehabilitation
- Spinal Cord Injury Research
- Nerve injury and regeneration
- Tendon Structure and Treatment
- Mesenchymal stem cell research
- Osteoarthritis Treatment and Mechanisms
- Neurogenesis and neuroplasticity mechanisms
- Orthopaedic implants and arthroplasty
- Pluripotent Stem Cells Research
- CAR-T cell therapy research
- Cellular Mechanics and Interactions
- Nanoplatforms for cancer theranostics
- Bone Tissue Engineering Materials
- Cervical and Thoracic Myelopathy
- Tissue Engineering and Regenerative Medicine
- TGF-β signaling in diseases
- Photodynamic Therapy Research Studies
- Cell death mechanisms and regulation
- Spinal Fractures and Fixation Techniques
- Immunotherapy and Immune Responses
- Connective tissue disorders research
- Pain Mechanisms and Treatments
- Circular RNAs in diseases
- Cancer-related molecular mechanisms research
Second Affiliated Hospital of Zhejiang University
2014-2024
Zhejiang University
2014-2024
Zhejiang Lab
2021-2024
Chinese Academy of Agricultural Sciences
2022-2024
Biotechnology Research Institute
2022-2024
Zhejiang University Medical College Affiliated Stomatological Hospital
2018-2022
Orthopaedic Research
2019
Illinois Mathematics and Science Academy
2012
The repair and motor functional recovery after spinal cord injury (SCI) remains a worldwide challenge. inflammatory microenvironment is one of main obstacles on inhibiting the SCI. Using mesenchymal stem cells (MSCs) derived extracellular vesicles to replace MSCs transplantation mimic cell paracrine secretions provides potential strategy for regulation. However, effective preservation controlled release in injured tissue are still not satisfied. Herein, we fabricated an injectable adhesive...
Abstract Osteosarcoma is one of the most malignant neoplasms in adolescents, and it generally develops multidrug resistance. Escin, a natural mixture triterpene saponins isolated from Aesculus hippocastanum (horse chestnut), has demonstrated potent anti-tumour potential vitro vivo . In present study, we found that escin inhibited osteosarcoma proliferation dose- time-dependent manner. Additionally, escin-induced apoptosis was evidenced by increased expression caspase-related proteins...
The current effective method for treatment of spinal cord injury (SCI) is to reconstruct the biological microenvironment by filling injured cavity area and increasing neuronal differentiation neural stem cells (NSCs) repair SCI. However, characterized several challenges including irregular wounds, mechanical electrical mismatch material-tissue interface. In study, a unique facile agarose/gelatin/polypyrrole (Aga/Gel/PPy, AGP3) hydrogel with similar conductivity modulus as was developed...
Abstract Background Human adipose-derived mesenchymal stem cells (ADMSCs) may be ideal source of for intervertebral disc (IVD) regeneration, but the harsh chemical microenvironment IVD significantly influence biological and metabolic vitality ADMSCs impair their repair potential. This study aimed to investigate viability, proliferation expression main matrix proteins in under normal degeneration conditions. Methods were harvested from young (aged 8-12 years, n = 6) mature 33-42 male donors...
The microenvironment of the intervertebral disc (IVD) is characterized by matrix acidity, hypoxia, hyperosmolarity and limited nutrition, which are major obstacles to stem cell-based regeneration. Our recent work showed that nucleus pulposus mesenchymal cells (NPMSCs) had advantages over traditional sources cell therapy under IVD-like hypoxic hyperosmotic conditions. Here, we examined viability, proliferation metabolism NPMSCs compared with adipose tissue-derived (ADMSCs) acidic conditions...
Chimeric antigen receptor (CAR)-engineered T cells have displayed outstanding performance in the treatment of patients with hematological malignancies. However, their efficacy against solid tumors has been largely limited.In this study, human osteosarcoma cell lines were prepared, flow cytometry using antibodies CD166 was performed on different samples. CD166-specific obtained by viral gene transfer corresponding DNA plasmids and selectively expanded IL-2 IL-15. The ability CD166.BBζ CAR-T...
Rationale: Spinal cord injury (SCI) remains a critical clinical challenge. The controlled release of FGF4, novel neuroprotective factor, from versatile Laponite hydrogel to the injured site was promising strategy promote axon regeneration and motor functional recovery after SCI. Methods: Characterization Laponite, Laponite/Heparin (Lap/Hep) loaded with FGF4 (Lap/Hep@FGF4) hydrogels were measured by rheometer. Multiple comprehensive evaluations used detect axonal rehabilitation Lap/Hep@FGF4...
Adipose-derived mesenchymal stem cells (ADMSCs) and nucleus pulposus-derived (NPMSCs) are two cell candidates for cell-based therapies intervertebral disc (IVD) regeneration. However, little work has been done to determine the influence of hypoxia in IVD on biological behaviors ADMSCs NPMSCs. This study aimed investigate viability, proliferation differentiation rat NPMSCs hypoxic environment vitro. isolated from 6 SD rats were cultured under normoxia (20% O2) (2% mimicking standard condition...
To examine the antitumor effects of gallic acid (GA) on osteosarcoma, two human osteosarcoma cell lines U-2OS and MNNG/HOS were treated by GA subjected to proliferation apoptosis assays. In addition, xenograft tumors established in nude BALB/c mice evaluate anticancer capacity vivo. The results showed that inhibited induced cells, accompanied upregulation p-38 activation downregulation c-Jun N-terminal kinase (JNK) extracellular signal regulated (ERK1/2) activation. Additionally, p38 MAPK...
Cell transplantation has been proved the promising therapeutic effects on intervertebral disc degeneration (IVDD). However, increased levels of reactive oxygen species (ROS) in degenerated region will impede efficiency human adipose-derived stem cells (human ADSCs) therapy. It inhibits ADSCs proliferation, and increases apoptosis. Herein, we firstly devised a novel amphiphilic copolymer PEG-PAPO, which could self-assemble into nanosized micelle load lipophilic kartogenin (KGN), as single...
Abstract Following severe spinal cord injury (SCI), dysregulated neuroinflammation causes neuronal and glial apoptosis, resulting in scar cystic cavity formation during wound healing ultimately the of an atrophic microenvironment that inhibits nerve regrowth. Because this complex dynamic pathophysiology, a systemic solution for scar‐ cavity‐free with remodeling to promote regrowth has rarely been explored. A one‐step is proposed through self‐assembling, multifunctional hydrogel depot...
Abstract Rejuvenation of nucleus pulposus cells (NPCs) in degenerative discs can reverse intervertebral disc degeneration (IDD). Partial reprogramming is used to rejuvenate aging and ameliorate progression tissue avoiding formation tumors by classical reprogramming. Understanding the effects potential mechanisms partial provides insights for development new therapies IDD treatment. The findings present study show that through short‐term cyclic expression Oct‐3/4, Sox2, Klf4, c‐Myc (OSKM)...
Adipose-derived stem cells (ADSCs) show great potential for the treatment of intervertebral disc (IVD) degeneration. An ideal carrier is necessary to transplant ADSCs into degenerated IVDs without influencing cell function. Nucleus pulposus (NPCs) can synthesize and deposit chondroitin sulfate type II collagen which are NP-specific extracellular matrix (ECM) also regulate differentiation cells. Bioscaffolds fabricated based on ECM synthesis functions NPCs have possible roles in...