A5100750876- Circular RNAs in diseases
- Cytokine Signaling Pathways and Interactions
- Cancer-related gene regulation
- RNA modifications and cancer
- Cancer, Hypoxia, and Metabolism
- Ubiquitin and proteasome pathways
- NF-κB Signaling Pathways
- Wnt/β-catenin signaling in development and cancer
- TGF-β signaling in diseases
- Cancer-related Molecular Pathways
- Epigenetics and DNA Methylation
- interferon and immune responses
- Protein Tyrosine Phosphatases
- Protein Kinase Regulation and GTPase Signaling
- Fibroblast Growth Factor Research
- Mitochondrial Function and Pathology
- PI3K/AKT/mTOR signaling in cancer
- MicroRNA in disease regulation
- Trace Elements in Health
- Pancreatic function and diabetes
- RNA Research and Splicing
- Immunotherapy and Immune Responses
- Genetic factors in colorectal cancer
- Bone Metabolism and Diseases
- Metabolism, Diabetes, and Cancer
Tsinghua University
2015-2025
Northwest A&F University
2025
State Key Laboratory of Membrane Biology
2017-2024
Jiaxing University
2024
Shanxi Medical University
2024
Center for Life Sciences
2022-2023
Putian University
2022
Shantou University
2019
Shantou University Medical College
2019
Delft University of Technology
2019
Abstract The mitochondrial GTPase mitofusin-2 (MFN2) has previously been reported to play a role in regulating cell proliferation, apoptosis and differentiation number of types. Here, we report that breast cancer patients with low MFN2 expression are associated poor prognosis as compared high expression. We find knockout from MCF7 A549 cells via Crispr/Cas9 greatly promotes viability, colony formation, invasion vitro vivo , which were confirmed by formation assay, transwell tumor xenograft...
Bone morphogenetic proteins (BMP) transduce their signals into the cell through a family of mediator known as Smads. Upon phosphorylation by BMP receptors, Smad1 interacts with Smad4 and translocates nucleus where complex recruits DNA-binding protein(s) to activate specific gene transcription. However, involved in signaling has not been identified. Using yeast two-hybrid approach, we found that Hoxc-8, homeodomain transcription factor. The interaction between Hoxc-8 was confirmed "pull-down"...
The severe acute respiratory syndrome (SARS) has been one of the most epidemic diseases threatening human health all over world. Based on clinical studies, SARS-CoV (the SARS-associated coronavirus), a novel coronavirus, is reported as pathogen responsible for disease. To date, no effective and specific therapeutic method can be used to treat patients suffering from infection. RNA interference (RNAi) process by which introduced small interfering (siRNA) could cause degradation mRNA with...
SARS-CoV (the SARS-Associated Coronavirus) was reported as a novel virus member in the coronavirus family, which cause of severe acute respiratory syndrome. Coronavirus replication occurs through unique mechanism employing Leader sequence transcripts when initiating transcription from genome. Therefore, we cloned SARS-CoV(BJ01), is identical to that identified SARS-CoV(HKU-39849), and constructed specific siRNA targeting sequence. Using EGFP RFP reporter genes fused with sequence,...
Runx2, an essential transactivator for osteoblast differentiation, is tightly regulated at both the transcriptional and posttranslational levels. In this paper, we report that CHIP (C terminus of Hsc70-interacting protein)/STUB1 regulates Runx2 protein stability via a ubiquitination-degradation mechanism. interacts with in vitro vivo. presence increased levels, expression decreases, whereas other E3 ligases involved degradation, such as Smurf1 or WWP1, remains constant increases during...
The carboxyl terminus of Hsc70-interacting protein (CHIP, also named Stub1), a U-box containing E3 ubiquitin ligase, is involved in degradation certain oncogenic proteins. Recent studies indicated that CHIP suppresses tumor progression human cancers by targeting Src-3, hypoxia inducible factor 1α, NF-κB, ErbB2 and c-Myc. Here, we report was downregulated, predominantly, the late stages colorectal cancer (CRC), promoter hypermethylated CRC specimens. Overexpression HCT-116 cells resulted...
Being relatively simple and practical, Drosophila transgenic RNAi is the technique of top priority choice to quickly study genes with pleiotropic functions. However, drawbacks have emerged over time, such as high level false positive negative results. To overcome these shortcomings increase efficiency, specificity versatility, we develop a next generation system. With this system, leaky expression basal promoter significantly reduced, well heterozygous ratio flies. In addition, it has been...
Cancers remain a threat to human health due the lack of effective therapeutic strategies.Great effort has been devoted discovery drug targets treat cancers, but novel oncoproteins still need be unveiled for efficient therapy.Methods: We show that CREPT is highly expressed in pancreatic cancer and associated with poor disease-free survival.CREPT overexpression promotes deletion blocks colony formation proliferation cells.To provide proof concept as new target inhibition cancer, we designed...
Immune checkpoint inhibitor (ICI) therapy is generating remarkable responses in individuals with cancer, but only a small portion of breast cancer respond well. Here we report that tumor-derived Jagged1 key regulator the tumor immune microenvironment. promotes tumorigenesis multiple spontaneous mammary models. Through Jagged1-induced Notch activation, cells increase expression and secretion cytokines to help recruit macrophages into Educated crosstalk tumor-infiltrating T inhibit cell...
Transforming growth factor beta (TGF-beta)/bone morphogenetic protein (BMP) family ligands interact with specific membrane receptor complexes that have serine/threonine kinase activities. The phosphorylation and activation induced by the leads to of Smad proteins, which translocate nucleus, controlling gene expression. Thus, regulation proteins is a key step in TGF-beta/BMP-induced signal transduction. Here we report novel mechanism SMAD-mediated signaling, Smad1 level controlled through...
Transforming growth factor-β (TGF-β) signaling is critical in a variety of biological processes such as cell proliferation, differentiation, and apoptosis. TGF-β mediated by group proteins including receptors Smads. It known that different cells can exhibit sensitivities to TGF-β. Several molecular mechanisms, the differential expression receptor levels, have been suggested contributing these differences. Here, we report evidence for novel mechanism regulating sensitivity depends on role...
The small GTPases Rab5 and Rab21 are closely related, play essential roles in endocytic trafficking. is regulated by VPS9-domain-containing guanine nucleotide exchange factors. Here, we describe a new VPS9-domain protein with ankyrin repeats, the VPS9-ankyrin-repeat (Varp). Varp interacts preferentially GDP-bound has much stronger activity towards than Rab5. Furthermore, RNAi-mediated depletion of endogenous significantly disrupts HeLa cells. Ectopically expressed mainly localizes to early...
Protein–protein interface design is one of the most exciting fields in protein science; however, designing nonnatural protein–protein interaction pairs remains difficult. In this article we report a de novo pair by scanning Protein Data Bank for suitable scaffold proteins that can be used grafting key residues and form stable complexes with target after additional mutations. Using our algorithm, an unrelated protein, rat PLCδ 1 -PH (pleckstrin homology domain phospholipase C-δ1), was...
The tumor suppressor p53 protein is tightly regulated by a ubiquitin-proteasomal degradation mechanism. Several E3 ubiquitin ligases, including MDM2 (mouse double minute 2), have been reported to play an essential role in the regulation of stability. However, it remains unclear how activity these ligases regulated. Here, we show that HECT-type ligase Smurf1/2 (Smad ubiquitylation regulatory factor 1/2) promotes enhancing MDM2. We provide evidence on stability not dependent but rather find...