A5100357222- interferon and immune responses
- SARS-CoV-2 and COVID-19 Research
- Poxvirus research and outbreaks
- Immune Cell Function and Interaction
- Bacillus and Francisella bacterial research
- Herpesvirus Infections and Treatments
- T-cell and B-cell Immunology
- Viral gastroenteritis research and epidemiology
- Animal Virus Infections Studies
- Muscle Physiology and Disorders
- RNA Research and Splicing
- Viral Infections and Immunology Research
- Immunotherapy and Immune Responses
- Adipose Tissue and Metabolism
- Plant Virus Research Studies
- Immune cells in cancer
- Meat and Animal Product Quality
- Kruppel-like factors research
- Genetic and phenotypic traits in livestock
- Animal Nutrition and Physiology
- Hepatitis B Virus Studies
- Hepatitis C virus research
- Reproductive System and Pregnancy
- Cardiomyopathy and Myosin Studies
- Osteoarthritis Treatment and Mechanisms
Sichuan University
2020-2025
Zhejiang Sci-Tech University
2025
West China Hospital of Sichuan University
2024
Zhongshan People's Hospital
2022-2024
Foshan University
2020-2024
National Institute for Viral Disease Control and Prevention
2016-2024
Jiangsu University
2021-2024
Affiliated Hospital of Jiangsu University
2021-2024
Second Military Medical University
2013-2023
Chinese Center For Disease Control and Prevention
2016-2023
Abstract SARS-CoV-2, a β-coronavirus, has rapidly spread across the world, highlighting its high transmissibility, but underlying morphogenesis and pathogenesis remain poorly understood. Here, we characterize replication dynamics, cell tropism of SARS-CoV-2 in organotypic human airway epithelial (HAE) cultures. replicates efficiently infects both ciliated secretory cells HAE In comparison, HCoV-NL63 to lower titers is only detected cells. shows similar morphogenetic process as other...
Reprogramming of NK cells with a chimeric antigen receptor (CAR) proved an effective strategy to increase cell reactivity and recognition specificity toward tumor cells. To enhance the cytotoxicity against CD138‐positive multiple myeloma (MM) cells, we generated genetically modified NK‐92MI carrying CAR that consists anti‐CD138 single‐chain variable fragment (scFv) fused CD3ζ chain as signaling moiety. The genetic modification through lentiviral vector did not affect intrinsic cytolytic...
The emergence of SARS-CoV-2 infection has posed unprecedented threat to global public health. virus-encoded non-structural protein 14 (nsp14) is a bi-functional enzyme consisting an exoribonuclease (ExoN) domain and methyltransferase (MTase) plays pivotal role in viral replication. Here, we report the structure nsp14-ExoN bound its co-factor nsp10 show that, compared SARS-CoV nsp10/nsp14-full-length complex, retains integral fold preserves active configuration catalytic center. Analysis...
•CD10+ALPL+ neutrophils contribute to tumor resistance anti-PD-1 treatment in patients with HCC.•CD10+ALPL+ exhibit immunosuppressive features the microenvironment.•CD10+ALPL+ trigger irreversible T-cell exhaustion anti-PD-1-resistant patients.•Hepatoma cell-derived NAMPT-NTRK1 axis reprograms CD10+ALPL+ maintain an immature and state. Background & AimsRemodeling microenvironment is a critical strategy for treating advanced hepatocellular carcinoma (HCC). Yet, how distinct cell populations...
Middle East respiratory syndrome coronavirus (MERS-CoV) is a novel and highly pathogenic human has quickly spread to other countries in the East, Europe, North Africa Asia since 2012. Previous studies have shown that MERS-CoV ORF4b antagonizes early antiviral alpha/beta interferon (IFN-α/β) response, which may significantly contribute pathogenesis; however, underlying mechanism poorly understood. Here, we found cytoplasm could specifically bind TANK binding kinase 1 (TBK1) IκB epsilon...
Cancer stem cell behavior is thought to be largely determined by intrinsic properties and regulatory signals provided the microenvironment. Myelofibrosis (MF) characterized hematopoiesis occurring not only in marrow but also extramedullary sites such as spleen. In order study effects of these different microenvironments on primitive malignant hematopoietic cells, we phenotypically functionally splenic peripheral blood (PB) MF CD34+ cells from patients with MF. spleens contained greater...
A novel human coronavirus, known as SARS-CoV-2 or 2019-nCoV, is the causative agent of coronavirus disease 2019 (COVID-19). We have released primers and probes real-time reverse transcription polymerase chain reaction (rRT-PCR) assays for laboratory detection COVID-19 infection.Here we provide detailed technical data evaluate performance three rRT-PCR targeting ORF1ab, N, E genes infection. The application among four types specimens (alveolar lavage, sputum, throat swabs, stool) from...
ABSTRACT Human coronaviruses (HCoVs) cause 15 to 30% of mild upper respiratory tract infections. However, no specific antiviral drugs are available prevent or treat HCoV infections date. Here, we developed four infectious recombinant HCoVs-OC43 (rHCoVs-OC43) which express the Renilla luciferase (Rluc) reporter gene. Among these rHCoVs-OC43, rOC43-ns2DelRluc (generated by replacing ns2 with Rluc gene) showed robust activity only a slight impact on its growth characteristics. Additionally,...
The persistent public health threat of infection with Middle East respiratory syndrome coronavirus (MERS-CoV) highlights the need for an effective and safe MERS-CoV vaccine. In this study, we prepared vaccinated mice either a Spike (S) protein or inactivated whole (IV) combined adjuvant (alum+CpG) as vaccine formulation. Similar levels anti-S IgG response neutralizing activity were induced by both S IV vaccines. addition, immune responses against three other structural proteins, envelope...
Abstract COVID-19 has become a global pandemic that threatens millions of people worldwide. There is an urgent call for developing effective drugs against the virus (SARS-CoV-2) causing this disease. The main protease SARS-CoV-2, 3C-like (3CL pro ), highly conserved across coronaviruses and essential maturation process viral polyprotein. Scutellariae radix (Huangqin in Chinese), root Scutellaria baicalensis been widely used traditional Chinese medicine to treat infection related symptoms....
A TCM formula has played an important role in the treatment of COVID-19 China. However, mechanism action is still unclear.
<abstract abstract-type="Abstract"> <sec> <b>Introduction</b> The first imported case of monkeypox (MPX) from the mainland China was reported in September 2022. Herein, study reports isolation and characterization MPX virus (MPXV) this case. </sec><sec> <b>Methods</b> Clinical specimens including skin blister fluid, oropharyngeal nasopharyngeal swabs, blood were collected inoculated onto Vero cells. isolated identified as MPXV using quantitative polymerase chain reaction (qPCR), cytopathic...
Lyssavirus glycoprotein plays a crucial role in mediating virus entry and serves as the major target for neutralizing antibodies. During membrane fusion, lyssavirus undergoes series of low-pH-induced conformational transitions. Here, we report structures Ikoma Mokola glycoproteins, with which believe that have trapped proteins pre-fusion post-fusion states respectively. By analyzing available lyssaviral structures, present sequential conformation-transition model, two structural elements...
Abstract Enveloped RNA viruses are a group of with an outer membrane derived from host cell and genome consisting ribonucleic acid (RNA). These rely on machinery organelles to replicate assemble new virus particles. However, the interaction between may be disrupted by nanomaterials, such as gold nanoparticles (AuNPs) unique physical chemical properties. In this study, we investigated effects AuNPs different surface charge properties subcellular structure function mammalian cells, their two...
Previous studies have indicated that bone morphogenetic protein (BMP) 6 may play an important role in skeletal system development and progression. However, the mechanism underlying effects of BMP6 cartilage cell proliferation differentiation remains unknown. In this study, cells were isolated from shanks chicken embryos treated with different concentrations Growth Hormone. Cell potential was assessed using real-time polymerase chain reaction (RT-PCR), western blotting CCK-8 assays vitro. The...
Abstract Monkeypox virus is a zoonotic disease endemic to Africa. In 2017, we confirmed case of human monkeypox in Sierra Leone by molecular and serologic methods. Sequencing analysis indicated the belongs West African clade data suggest it was likely transmitted wild animals.