A5039903670- Acute Ischemic Stroke Management
- Intracerebral and Subarachnoid Hemorrhage Research
- Long-Term Effects of COVID-19
- Neurological Disease Mechanisms and Treatments
- SARS-CoV-2 and COVID-19 Research
- Animal Virus Infections Studies
- COVID-19 Clinical Research Studies
- Parkinson's Disease Mechanisms and Treatments
- Neuroinflammation and Neurodegeneration Mechanisms
- Viral gastroenteritis research and epidemiology
- Heme Oxygenase-1 and Carbon Monoxide
- Cerebrovascular and Carotid Artery Diseases
- Virus-based gene therapy research
- Neuroscience and Neuropharmacology Research
- Tryptophan and brain disorders
- Traditional Chinese Medicine Analysis
- Neurological Disorders and Treatments
- Antimicrobial Resistance in Staphylococcus
- Advanced Neuroimaging Techniques and Applications
- Connexins and lens biology
- Direction-of-Arrival Estimation Techniques
- COVID-19 and Mental Health
- Indoor and Outdoor Localization Technologies
- Thermal Regulation in Medicine
- Cancer Genomics and Diagnostics
Yangzhou University
2014-2025
Northern Jiangsu People's Hospital
2015-2025
Peking University
2019-2025
Peking University Cancer Hospital
2019-2025
Dalian Institute of Chemical Physics
2022-2023
Chinese Academy of Sciences
2022-2023
Shenzhen University
2023
First People's Hospital of Chongqing
2023
Guangxi University of Chinese Medicine
2022
Chongqing Medical University
2018-2020
Abstract Most neutralizing antibodies against Middle East respiratory syndrome coronavirus (MERS-CoV) target the receptor-binding domain (RBD) of spike glycoprotein and block its binding to cellular receptor dipeptidyl peptidase 4 (DPP4). The epitopes mechanisms mAbs targeting non-RBD regions have not been well characterized yet. Here we report monoclonal antibody 7D10 that binds N-terminal (NTD) inhibits cell entry MERS-CoV with high potency. Structure determination mutagenesis experiments...
Abstract Introduction Vascular cognitive impairment without dementia is very common among the aged and tends to progress dementia, but there have been no proper large‐scale intervention trials dedicated it. caused by subcortical ischemic small vessel disease (hereinafter, dementia) represents a relatively homogeneous process suitable target for therapeutic investigating dementia. Preclinical showed that dl‐3‐n‐butylphthalide (NBP) effective of vascular origin. Methods In this randomized,...
Xanthine oxidase (XO), a form of xanthine oxidoreductase, is widely distributed in various human tissues. As major source for the generation superoxide radicals, XO involved induction oxidative stress and inflammation during ischemic hypoxic tissue injury. Therefore, we designed this study to identify role serum levels acute stroke (AIS) pathogenesis. In single-center prospective study, 328 consecutive patients with AIS first time were included, 107 age- sex-matched healthy controls from...
The persistent public health threat of infection with Middle East respiratory syndrome coronavirus (MERS-CoV) highlights the need for an effective and safe MERS-CoV vaccine. In this study, we prepared vaccinated mice either a Spike (S) protein or inactivated whole (IV) combined adjuvant (alum+CpG) as vaccine formulation. Similar levels anti-S IgG response neutralizing activity were induced by both S IV vaccines. addition, immune responses against three other structural proteins, envelope...
Cerebral ischaemia/reperfusion (CI/R) injury is a major challenge due to the lack of effective neuroprotective drugs. Hederagenin (HE) aglycone part saponins extracted from Hedera helix Linné that has exhibited anti-apoptotic and anti-inflammatory effects; however, role HE in CI/R not been elucidated. In this study, mice were intraperitoneally (i.p.) injected with (26.5, 53, or 106 μmol/kg body weight) for 3 days after middle cerebral artery occlusion (MCAO). Neural function brain infarct...
Connexin 43(Cx43) widely exists in all components of the neurovascular unit and is a constituent gap junctions hemichannels. In physiological states, are open for regular intercellular communication, hemichannels present low probability astrocytes. After cerebral ischemia, large number unusually opened, leading to cell swelling even death. Most known hemichannel blockers also inhibit sequentially obstruct normal electrical cell-cell communication. this study, we tested hypothesis that Gap19,...
Abstract The combination of super‐resolution microscopy and synthetic fluorescence probes has emerged as a universal tool to monitor dynamic biological events at the nanometer scale. However, limited site‐specificity fluorogenicity fluorescent make it still difficult realize long‐term imaging. Herein, we introduce aggregation mediated SNAP‐tag fluorogenic probe, BGAN‐Aze , which can specifically bind various fusion proteins with 41‐fold enhancement. equilibrium between non‐fluorescent...
A blood-based approach to monitor metastasis and recurrence of esophageal squamous cell carcinoma (ESCC) remains undeveloped. This study aimed establish a dependable model utilizing cfDNA 5-hydroxymethylcytosines (5hmC) signatures detect these conditions in ESCC. The 5hmC-Seal technique was employed generate comprehensive 5hmC profiles from the plasma cell-free DNA (cfDNA) 122 ESCC patients, classified into 72 with metastasis, 50 without 30 recurrence, 92 recurrence. Initial steps involved...
Background: Parkinson's disease (PD), a prevalent and progressive neurodegenerative disorder, currently lacks effective satisfactory pharmacological treatments. Computational drug repurposing represents promising efficient strategy for discovery, aiming to identify new therapeutic indications existing pharmaceuticals. Methods: We employed drug-target network approach computationally repurpose FDA-approved drugs from databases such as DrugBank. A literature review was conducted select...
Middle East respiratory syndrome coronavirus (MERS-CoV) has caused fatal infections, some through hospital-acquired transmission, in affected regions since its emergence 2012. Although the virus is not pandemic among humans, it poses a great threat to public health due zoonotic origin. Thus, both preventative and therapeutic countermeasures are urgently needed. In this study, we discovered panel of neutralizing monoclonal antibodies (mAbs) against MERS-CoV, which mapped wide range on spike...
The mechanisms of age-associated memory impairment may be associated with glutamate receptor function and chromatin modification. To observe the effect an enriched environment on cognitive mice impairment, 3-month-old C57BL/6 male ("young" mice) were raised in a standard environment, while 24-month-old ("age-associated impairment" either or environment. included variety stimuli involving movement sensation. A water maze test was then used to measure mice. Furthermore, quantitative real-time...
Despite recent recognition of the ATP-binding cassette protein OptrA as an important mediator linezolid resistance in Enterococcus faecalis worldwide, mechanisms optrA gene acquisition and transfer remain poorly understood. In this study, we performed comprehensive molecular phenotypic profiling 44 optrA-carrying E. clinical isolates with resistance. Pulse-field gel electrophoresis DNA hybridization revealed presence plasmid 26 (59%) chromosome 18 (41%) isolates. Conjugation experiments...