A5003336327- HIV Research and Treatment
- SARS-CoV-2 and COVID-19 Research
- HIV/AIDS drug development and treatment
- HIV/AIDS Research and Interventions
- Immune Cell Function and Interaction
- COVID-19 Clinical Research Studies
- Monoclonal and Polyclonal Antibodies Research
- Animal Virus Infections Studies
- Hepatitis C virus research
- Viral gastroenteritis research and epidemiology
- T-cell and B-cell Immunology
- Influenza Virus Research Studies
- SARS-CoV-2 detection and testing
- vaccines and immunoinformatics approaches
- Immunotherapy and Immune Responses
- Bacteriophages and microbial interactions
- Hepatitis B Virus Studies
- Virology and Viral Diseases
- Respiratory viral infections research
- HIV, Drug Use, Sexual Risk
- COVID-19 epidemiological studies
- Viral Infections and Vectors
- Cytomegalovirus and herpesvirus research
- Virus-based gene therapy research
- Mosquito-borne diseases and control
Tsinghua University
2016-2025
Emory University
1999-2025
Shenzhen Bay Laboratory
2021-2025
Tsinghua–Berkeley Shenzhen Institute
2021-2025
Peking University
2019-2025
Nanjing University of Aeronautics and Astronautics
2023-2024
First Affiliated Hospital of Henan University
2021-2024
Guangzhou Medical University
2015-2023
Peking University People's Hospital
2020-2023
First Affiliated Hospital of Henan University of Traditional Chinese Medicine
2022-2023
A new and highly pathogenic coronavirus (severe acute respiratory syndrome coronavirus-2, SARS-CoV-2) caused an outbreak in Wuhan city, Hubei province, China, starting from December 2019 that quickly spread nationwide to other countries around the world1-3. Here, better understand initial step of infection at atomic level, we determined crystal structure receptor-binding domain (RBD) spike protein SARS-CoV-2 bound cell receptor ACE2. The overall ACE2-binding mode RBD is nearly identical...
To determine the role of CD8(+) T cells in controlling simian immunodeficiency virus (SIV) replication vivo, we examined effect depleting this cell population using an anti-CD8 monoclonal antibody, OKT8F. There was on average a 99.9% reduction CD8 peripheral blood six infected Macaca mulatta treated with The apparent depletion started 1 h after antibody administration, and low levels were maintained until day 8. An increase plasma viremia one to three orders magnitude observed five macaques....
<h3>Importance</h3> Convalescent plasma is a potential therapeutic option for patients with coronavirus disease 2019 (COVID-19), but further data from randomized clinical trials are needed. <h3>Objective</h3> To evaluate the efficacy and adverse effects of convalescent therapy COVID-19. <h3>Design, Setting, Participants</h3> Open-label, multicenter, trial performed in 7 medical centers Wuhan, China, February 14, 2020, to April 1, final follow-up 28, 2020. The included 103 participants...
In most subjects infected with human immunodeficiency virus type 1 (HIV-1), clinical or laboratory evidence of develops within 10 years seroconversion, but a few people remain healthy and immunologically normal for more than decade. Studies these subjects, termed long-term survivors, may yield important clues the development prophylactic therapeutic interventions against acquired syndrome.
In patients infected with human immunodeficiency virus type 1 (HIV-1), combination antiretroviral therapy can result in sustained suppression of plasma levels the virus. However, replication-competent still be recovered from latently resting memory CD4 lymphocytes; this finding raises serious doubts about whether antiviral treatment eradicate HIV-1.
The spike glycoprotein (S) of recently identified Middle East respiratory syndrome coronavirus (MERS-CoV) targets the cellular receptor, dipeptidyl peptidase 4 (DPP4). Sequence comparison and modeling analysis have revealed a putative receptor-binding domain (RBD) on viral spike, which mediates this interaction. We report 3.0 Å-resolution crystal structure MERS-CoV RBD bound to extracellular human DPP4. Our results show that consists core subdomain. subdomain interacts with DPP4 β-propeller...
It has been known since 1986 that CD8 T lymphocytes from certain HIV-1–infected individuals who are immunologically stable secrete a soluble factor, termed CAF, suppresses HIV-1 replication. However, the identity of CAF remained elusive despite an extensive search. By means protein-chip technology, we identified cluster proteins were secreted when cells long-term nonprogressors with infection stimulated. These as α-defensin 1, 2, and 3 on basis specific antibody recognition amino acid...
ABSTRACT The shedding of severe acute respiratory syndrome coronavirus (SARS-CoV) into saliva droplets plays a critical role in viral transmission. source high loads saliva, however, remains elusive. Here we investigate the early target cells infection entire array tissues Chinese macaques after intranasal inoculations with single-cycle pseudotyped virus and pathogenic SARS-CoV. We found that angiotensin-converting enzyme 2-positive (ACE2 + ) were widely distributed upper tract, ACE2...
The 501Y.V2 variants of SARS-CoV-2 containing multiple mutations in spike are now dominant South Africa and rapidly spreading to other countries. Here, experiments with 18 pseudotyped viruses showed that the do not confer increased infectivity cell types except for murine ACE2-overexpressing cells, where a substantial increase was observed. Notably, susceptibility 12 17 neutralizing monoclonal antibodies substantially diminished, neutralization ability sera from convalescent patients...
Abstract The pandemic caused by emerging coronavirus SARS-CoV-2 presents a serious global public health emergency in urgent need of prophylactic and therapeutic interventions. cellular entry depends on binding between the viral Spike protein receptor-binding domain (RBD) angiotensin converting enzyme 2 (ACE2) target cell receptor. Here, we report isolation characterization 206 RBD-specific monoclonal antibodies (mAbs) derived from single B cells eight infected individuals. These mAbs come...
Human neutralizing monoclonal antibody could serve as a therapeutic intervention against MERS-CoV infection.
Abstract A novel and highly pathogenic coronavirus (2019-nCoV) has caused an outbreak in Wuhan city, Hubei province of China since December 2019, soon spread nationwide spilled over to other countries around the world. To better understand initial step infection at atomic-level, we determined crystal structure 2019-nCoV spike receptor-binding domain (RBD) bound with cell receptor ACE2 2.45 Å resolution. The overall ACE2-binding mode RBD is nearly identical that SARS-CoV RBD, which also...
Abstract Most neutralizing antibodies against Middle East respiratory syndrome coronavirus (MERS-CoV) target the receptor-binding domain (RBD) of spike glycoprotein and block its binding to cellular receptor dipeptidyl peptidase 4 (DPP4). The epitopes mechanisms mAbs targeting non-RBD regions have not been well characterized yet. Here we report monoclonal antibody 7D10 that binds N-terminal (NTD) inhibits cell entry MERS-CoV with high potency. Structure determination mutagenesis experiments...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) causes the global pandemic of COVID-19. SARS-CoV-2 is classified as a biosafety level-3 (BSL-3) agent, impeding basic research into its biology and development effective antivirals. Here, we developed level-2 (BSL-2) cell culture system for production transcription replication-competent virus-like-particles (trVLP). This trVLP expresses reporter gene (GFP) replacing viral nucleocapsid (N), which required genome packaging virion...
Abstract Understanding the mechanism for antibody neutralization of SARS-CoV-2 is critical development effective therapeutics and vaccines. We recently isolated a large number monoclonal antibodies from infected individuals. Here we select top three most potent yet variable neutralizing in-depth structural functional analyses. Crystal comparisons reveal differences in angles approach to receptor binding domain (RBD), size buried surface areas, key residues on RBD viral spike glycoprotein....
Abstract Neutralizing antibodies (nAbs) to SARS-CoV-2 hold powerful potentials for clinical interventions against COVID-19 disease. However, their common genetic and biologic features remain elusive. Here we interrogate a total of 165 from eight patients, find that potent nAbs different patients have disproportionally high representation IGHV3-53/3-66 usage, therefore termed as public antibodies. Crystal structural comparison these reveals they share similar angle approach RBD, overlap in...
Abstract In recognizing the host cellular receptor and mediating fusion of virus cell membranes, spike (S) glycoprotein coronaviruses is most critical viral protein for cross-species transmission infection. Here we determined cryo-EM structures spikes from bat (RaTG13) pangolin (PCoV_GX) coronaviruses, which are closely related to SARS-CoV-2. All three receptor-binding domains (RBDs) these two trimers in “down” conformation, indicating they more prone adopt inactive state. However, found...