A5100817777- Influenza Virus Research Studies
- interferon and immune responses
- Respiratory viral infections research
- Acute Ischemic Stroke Management
- Ubiquitin and proteasome pathways
- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- Viral gastroenteritis research and epidemiology
- Cancer-related gene regulation
- Diet and metabolism studies
- Evolution and Genetic Dynamics
- Epigenetics and DNA Methylation
- Venous Thromboembolism Diagnosis and Management
- RNA Research and Splicing
- Liver Disease Diagnosis and Treatment
- Animal Disease Management and Epidemiology
- Endoplasmic Reticulum Stress and Disease
- Protein Structure and Dynamics
- Molecular Biology Techniques and Applications
- Cancer, Hypoxia, and Metabolism
- NF-κB Signaling Pathways
- Food Quality and Safety Studies
- Fungal and yeast genetics research
- Traumatic Brain Injury and Neurovascular Disturbances
- Heat shock proteins research
Chinese Academy of Agricultural Sciences
2016-2025
Harbin Veterinary Research Institute
2016-2025
Affiliated Hospital of Southwest Medical University
2022-2024
Xinjiang Institute of Ecology and Geography
2024
Chinese Academy of Sciences
2024
Northern Jiangsu People's Hospital
2023
Yangzhou University
2021-2023
Fuzhou Maternity and Child Health Care Hospital
2023
Wuxi People's Hospital
2023
East China University of Technology
2023
A newly emerged H7N9 virus has caused 132 human infections with 37 deaths in China since 18 February 2013. Control measures virus-positive live poultry markets have reduced the number of infections; however, character virus, including its pandemic potential, remains largely unknown. We systematically analyzed viruses isolated from birds and humans. The were genetically closely related bound to airway receptors; some also maintained ability bind avian receptors. nonpathogenic chickens, ducks,...
Certain low pathogenic avian influenza viruses can mutate to highly when they circulate in domestic poultry, at which point cause devastating poultry diseases and severe economic damage. The H7N9 that emerged 2013 China had caused human infections deaths. However, these were nonlethal poultry. It is unknown whether the acquire additional mutations during their circulation nature become lethal more dangerous for humans. Here, we evaluated evolution of isolated from species between 2017 found...
Tirofiban is a highly selective nonpeptide antagonist of glycoprotein IIb/IIIa receptor, which reversibly inhibits platelet aggregation. It remains uncertain whether intravenous tirofiban effective to improve functional outcomes for patients with large vessel occlusion ischemic stroke undergoing endovascular thrombectomy.To assess the efficacy and adverse events before thrombectomy acute secondary occlusion.This investigator-initiated, randomized, double-blind, placebo-controlled trial was...
Significance The aryl hydrocarbon receptor (AHR) is unique among PAS family transcription factors in its diverse environmental and cellular ligands broad array of biological endpoints. AHR dimerizes with nuclear translocator (ARNT) subsequently interacts genomic enhancers, dioxin response elements (DREs). Our crystal structure the AHR–ARNT heterodimer complex DRE provides structural insights into this transcriptional complex. studies reveal three-dimensional codes for specific engagement...
Abstract Tripartite motif (TRIM) family proteins are important effectors of innate immunity against viral infections. Here we identified TRIM35 as a regulator TRAF3 activation. Deficiency in or inhibition suppressed the production type I interferon (IFN) response to infection. Trim35 -deficient mice were more susceptible influenza A virus (IAV) infection than wild-type mice. promoted RIG-I-mediated signaling by catalyzing Lys63-linked polyubiquitination and subsequent formation complex with...
Lethal infection of wild birds with different subtypes H5 viruses continuously occur. To investigate the genetic evolution and pathogenicity in birds, we performed a detailed biologic analysis 27 viruses, including H5N1, H5N2, H5N6, H5N8 subtypes, that were responsible for avian influenza outbreaks China over past decade. We found these bearing clades/subclades HA, complicated reassortants formed 12 genotypes. Ten tested highly pathogenic chickens, but showed distinct pathotypes ducks mice....
Xanthine oxidase (XO), a form of xanthine oxidoreductase, is widely distributed in various human tissues. As major source for the generation superoxide radicals, XO involved induction oxidative stress and inflammation during ischemic hypoxic tissue injury. Therefore, we designed this study to identify role serum levels acute stroke (AIS) pathogenesis. In single-center prospective study, 328 consecutive patients with AIS first time were included, 107 age- sex-matched healthy controls from...
Abstract The global dissemination of H5 avian influenza viruses represents a significant threat to both human and animal health. In this study, we conducted genome‐wide siRNA library screening against the highly pathogenic H5N1 virus, leading us identification 457 cellular cofactors (441 proviral factors 16 antiviral factors) involved in virus replication cycle. Gene Ontology term enrichment analysis revealed that candidate gene data sets were enriched categories associated with mRNA...
In natural systems, selection acts on both protein sequence and expression level, but it is unclear how integrates over these two dimensions. We recently developed the EMPIRIC approach to systematically determine fitness effects of all possible point mutants for important regions essential genes in yeast. Here, we investigated mutations a putative substrate binding loop yeast Hsp90 (Hsp82) broad range strengths. Negative epistasis between reduced strength amino acid substitutions was common,...
Proper activation of protein phosphatase 2A (PP2A) catalytic subunit is central for the complex PP2A regulation and crucial broad aspects cellular function. The crystal structure bound to activator (PTPA) ATPγS reveals that PTPA makes contacts with structural elements surrounding active site adenine moiety ATP. PTPA-binding stabilizes fold apo-PP2A required activation, orients ATP phosphoryl groups bind directly site. This allows modulate metal-binding preferences utilize hydrolysis. In...
Transcription and replication of the influenza A virus (IAV) genome occur in nucleus infected cells are carried out by viral ribonucleoprotein complex (vRNP). As a major component vRNP complex, nucleoprotein (NP) mediates nuclear import via its localization signals (NLSs). Clearly, an effective way for host to antagonize IAV infection would be targeting import. Here, we identified phospholipid scramblase 1 (PLSCR1) as binding partner NP using yeast two-hybrid (Y2H) screen. The interaction...
The emergence of the PB2 E627K substitution is critical in mammalian adaptation and pathogenesis AIV. H7N9 AIVs that emerged 2013 possess a prominent ability gaining mutation humans. Here, we demonstrate acquisition driven by low polymerase activity conferred viral PA protein human cells, four residues are collectively involved this process. Notably, leads to significant dependence function on ANP32A protein, Anp32a knockout abolishes mice. These findings reveal host crucial for during
The B″/PR72 family of protein phosphatase 2A (PP2A) is an important PP2A involved in diverse cellular processes, and uniquely regulated by calcium binding to the regulatory subunit. PR70 subunit this interacts with cell division control 6 (Cdc6), a cycle regulator for DNA replication. Here, we report crystal structures isolated PR72 trimeric holoenzyme at resolution 2.1 2.4 Å, respectively, vitro characterization Cdc6 dephosphorylation. structure reveals that one calcium-binding motifs...
To complete its replication cycle, IAV hijacks the host endocytosis machinery to invade cells. However, underlying mechanisms of how is internalized into cells remain poorly understood, emphasizing need elucidate role factors in entry In this study, we identified FFAR2 as an important factor for efficient both low-pathogenic and highly pathogenic IAV. We revealed that facilitates internalization target during early stage infection. Upon further characterization FFAR2-associated proteins...
Host defense systems employ posttranslational modifications to protect against invading pathogens. Here, we found that protein inhibitor of activated STAT 1 (PIAS1) interacts with the nucleoprotein (NP), polymerase basic (PB1), and 2 (PB2) influenza A virus (IAV). Lentiviral-mediated stable overexpression PIAS1 dramatically suppressed replication IAV, whereas siRNA knockdown or CRISPR/Cas9 knockout expression significantly increased growth. The was induced upon IAV infection in both cell...
The catalytic subunit of protein phosphatase 2A (PP2Ac) is stabilized in a latent form by α4, regulatory essential for cell survival and biogenesis all PP2A complexes. Here we report the structure α4 bound to N-terminal fragment PP2Ac. This suggests that binding full-length PP2Ac requires local unfolding near active site, which perturbs scaffold site at opposite surface via allosteric relay. These changes stabilize an inactive conformation convert oligomeric complexes complex upon...
The aryl hydrocarbon receptor (AHR) is a transcription factor that responds to diverse ligands and plays critical role in toxicology, immune function, cardiovascular physiology. structural basis of the AHR for ligand promiscuity preferences understanding function. Based on structure closely related protein HIF2α, we modeled binding domain (LBD) bound 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) benzo(a)pyrene (BaP) identified residues control by shape H-bond potential. Mutations these...
Abstract As a major component of the viral ribonucleoprotein (vRNP) complex in influenza A virus (IAV), nucleoprotein (NP) interacts with isoforms importin α family members, leading to import itself and vRNP into nucleus, process pivotal replication cycle IAV. In this study, we found that BinCARD1, an isoform Bcl10-interacting protein CARD (BinCARD), was leveraged by IAV for efficient replication. BinCARD1 promoted nuclear newly synthesized NP thus enhanced activity. Moreover, interacted...
ABSTRACT SLC35B4, a nucleotide sugar transporter that mediates the transport of UDP-GlcNAc and UDP-xylose, was found to be required for replication influenza A virus (IAV) H5N1 subtype in our genome-wide siRNA library screen. We defective IAV SLC35B4-deficient A549 cells independent strain specificity, virulence Slc35b4 knockdown mice also decreased. By examining individual stages cycle, we discovered amount internalized significantly reduced SLC35B4-knockout cells. Mechanistically, SLC35B4...