A5100454863- Influenza Virus Research Studies
- Respiratory viral infections research
- Animal Disease Management and Epidemiology
- interferon and immune responses
- Viral gastroenteritis research and epidemiology
- Animal Virus Infections Studies
- Immune Response and Inflammation
- RNA and protein synthesis mechanisms
- Virus-based gene therapy research
- Liver Disease Diagnosis and Treatment
- RNA Research and Splicing
- Protein Structure and Dynamics
- Immune Cell Function and Interaction
- Cytokine Signaling Pathways and Interactions
- Hepatocellular Carcinoma Treatment and Prognosis
- Viral Infections and Vectors
- Cancer, Stress, Anesthesia, and Immune Response
- Liver Disease and Transplantation
- Head and Neck Surgical Oncology
- Ubiquitin and proteasome pathways
- RNA modifications and cancer
- Gas Sensing Nanomaterials and Sensors
- Endoplasmic Reticulum Stress and Disease
- Covalent Organic Framework Applications
- Cell death mechanisms and regulation
Harbin Veterinary Research Institute
2016-2025
Chinese Academy of Agricultural Sciences
2016-2025
First Affiliated Hospital of Xi'an Jiaotong University
2023
Wenzhou Medical University
2022-2023
First Affiliated Hospital of Wenzhou Medical University
2022-2023
Key Laboratory of Guangdong Province
2021-2023
Sichuan University
2023
University of Hong Kong
2022
Thermal Technologies (United States)
2022
Hunan Normal University
2022
Highly pathogenic avian H5N1 influenza A viruses occasionally infect humans, but currently do not transmit efficiently among humans. The viral haemagglutinin (HA) protein is a known host-range determinant as it mediates virus binding to host-specific cellular receptors. Here we assess the molecular changes in HA that would allow possessing subtype H5 be transmissible mammals. We identified reassortant HA/H1N1 virus-comprising (from an virus) with four mutations and remaining seven gene...
A newly emerged H7N9 virus has caused 132 human infections with 37 deaths in China since 18 February 2013. Control measures virus-positive live poultry markets have reduced the number of infections; however, character virus, including its pandemic potential, remains largely unknown. We systematically analyzed viruses isolated from birds and humans. The were genetically closely related bound to airway receptors; some also maintained ability bind avian receptors. nonpathogenic chickens, ducks,...
Two amino acids (lysine at position 627 or asparagine 701) in the polymerase subunit PB2 protein are considered critical for adaptation of avian influenza A viruses to mammals. However, recently emerged pandemic H1N1 lack these acids. Here, we report that a basic acid 591 can compensate lysine and confers efficient viral replication Moreover, substantially increased lethality an H5N1 virus mice. We also present X-ray crystallographic structure C-terminus protein. Arginine fills cleft found...
The broad range and diversity of interferon-stimulated genes (ISGs) function to induce an antiviral state within the host, impeding viral pathogenesis. While successful respiratory viruses overcome individual ISG effectors, analysis global response subsequent antagonism has yet be examined. Employing models human airway, transcriptomics proteomics datasets were used compare patterns following highly pathogenic H5N1 avian influenza (HPAI) A virus, 2009 pandemic H1N1, severe acute syndrome...
Certain low pathogenic avian influenza viruses can mutate to highly when they circulate in domestic poultry, at which point cause devastating poultry diseases and severe economic damage. The H7N9 that emerged 2013 China had caused human infections deaths. However, these were nonlethal poultry. It is unknown whether the acquire additional mutations during their circulation nature become lethal more dangerous for humans. Here, we evaluated evolution of isolated from species between 2017 found...
H5N1 avian influenza viruses bearing the clade 2.3.4.4b hemagglutinin gene have been widely circulating in wild birds and are responsible for loss of over 70 million domestic poultry Europe, Africa, Asia, North America since October 2020. During our routine surveillance, 13 were isolated from 26,767 bird samples that collected between September 2021 March 2022 China. To investigate origin these Chinese isolates understand their genetic relationship with globally viruses, we performed a...
The globally circulating H5N8 avian influenza viruses bearing the clade 2.3.4.4b hemagglutinin (HA) gene are responsible for loss of more than 33 million domestic poultry since January 2020. Moreover, have reassorted with other and formed H5N1, H5N2, H5N3, H5N4, H5N5 in Europe, Africa, North America. In this study, we analyzed 15 H5N6 isolated from seven humans, found these different genotypes by deriving HA viruses, neuraminidase duck internal genes that previously circulated ducks wild...
In mouse models of influenza, T cells can confer broad protection against multiple viral subtypes when antibodies raised a single subtype fail to do so. However, the role in protecting humans influenza remains unclear. Here we employ translational nonhuman primate model show that cross-reactive cell responses play an important early clearance infection with 2009 pandemic H1N1 virus (H1N1pdm). To “prime” cellular immunity, first infected 5 rhesus macaques seasonal human isolate. These animals...
The spread of avian H5N1 influenza viruses around the globe has become a worldwide public health concern. To evaluate pathogenic potential reassortant between currently cocirculating and human H3N2 viruses, we generated all 254 combinations A/chicken/South Kalimantan/UT6028/06 (SK06, H5N1) A/Tokyo/Ut-Sk-1/07 (Tok07, H3N2) by reverse genetics. We found that presence Tok07 PB2 protein in ribonucleoprotein (RNP) complex allowed efficient viral RNA transcription minigenome assay RNP activity...
Engineered SH2 domains with high affinity for phosphorylated tyrosine inhibit cell signaling downstream of receptor kinases.
Influenza viruses of the H6 subtype have been isolated from wild and domestic aquatic terrestrial avian species throughout world since their first detection in a turkey Massachusetts 1965. Since 1997, with different neuraminidase (NA) subtypes detected frequently live poultry markets southern China. Although sequence information has gathered over last few years, not fully biologically characterized. To investigate potential risk posed by to humans, here we assessed receptor-binding...
Significance Animal influenza viruses continue to challenge human public health; surveillance of these and evaluation their pandemic potential are important for future preparedness. Pigs intermediate hosts novel generation transfer humans. Two lineages H1N1 swine (SIVs), classical SIVs Eurasian avian-like (EAH1N1) SIVs, have been circulating in pigs since 1918 1979, respectively. The emerged humans as a reassortant caused the 2009 pandemic. Here, we found that, after long-term evolution...
The H7N9 avian influenza virus (AIV) that emerged in China have caused five waves of human infection. Further cases been successfully prevented since September 2017 through the use an vaccine poultry. However, AIV has not eradicated from poultry China, and its evolution remains largely unexplored. In this study, we isolated 19 AIVs during surveillance diagnosis February 2018 to December 2019, genetic analysis showed these viruses formed two different genotypes. Animal studies indicated are...
Abstract Tripartite motif (TRIM) family proteins are important effectors of innate immunity against viral infections. Here we identified TRIM35 as a regulator TRAF3 activation. Deficiency in or inhibition suppressed the production type I interferon (IFN) response to infection. Trim35 -deficient mice were more susceptible influenza A virus (IAV) infection than wild-type mice. promoted RIG-I-mediated signaling by catalyzing Lys63-linked polyubiquitination and subsequent formation complex with...
The H7N9 influenza virus emerged in China 2013, causing more than 1560 human infections, 39% of which were fatal. A 'cytokine storm' the lungs patients has been linked to a poor prognosis and death; however, underlying mechanism that triggers cytokine storm is unknown. Here, we found efficient replication mouse activates gasdermin E (GSDME)-mediated pyroptosis alveolar epithelial cells, released cytosolic contents then trigger storm. Knockout Gsdme switched manner death A549 primary cells...
Lethal infection of wild birds with different subtypes H5 viruses continuously occur. To investigate the genetic evolution and pathogenicity in birds, we performed a detailed biologic analysis 27 viruses, including H5N1, H5N2, H5N6, H5N8 subtypes, that were responsible for avian influenza outbreaks China over past decade. We found these bearing clades/subclades HA, complicated reassortants formed 12 genotypes. Ten tested highly pathogenic chickens, but showed distinct pathotypes ducks mice....