Alzheimer's disease (AD) is a prevalent and progressive neurodegenerative disorder that the leading cause of dementia. The underlying mechanisms AD have not yet been completely explored. Neuroinflammation, an inflammatory response mediated by certain mediators, has exhibited to play crucial role in pathogenesis AD. Additionally, disruption gut microbiota found be associated with AD, fecal transplantation (FMT) emerged as potential therapeutic approach. However, precise mechanism FMT...

Accurate, rapid, and sensitive pathogenic detections play an important role in food safety. Herein, we developed a novel CRISPR/Cas12a mediated strand displacement/hybridization chain reaction (CSDHCR) nucleic acid assay for foodborne colorimetric detection. A biotinylated DNA toehold is coupled on avidin magnetic beads acts as initiator to trigger the SDHCR. The SDHCR amplification allowed formation of long hemin/G-quadruplex-based DNAzyme products catalyze TMB-H2O2 reaction. In presence...

Colorectal cancer (CRC) ranks among the most prevalent malignant tumors globally. Recent reports suggest that

Abstract CRISPR-Cas12a, an RNA-guided nuclease, has been repurposed for genome editing and molecular diagnostics due to its capability of cis-cleavage on target DNA trans-cleavage non-target single-strand (ssDNA). However, the mechanisms underlying activation activity Cas12a, particularly in context split activators, remain poorly understood. We elucidate synergistic effect these activators introduce concepts induced targeting exon-unwinding describe phenomenon. demonstrate that upon binding...

ABSTRACT Cyclic di-AMP (c-di-AMP) is a recently discovered second messenger in bacteria. Most of work on c-di-AMP signaling has been done Gram-positive bacteria, firmicutes, and actinobacteria, where pathways affect potassium transport, cell wall structure, antibiotic resistance. Little known about other Borrelia burgdorferi , the causative agent Lyme disease, spirochete that Gram-negative dual membrane. In this study, we demonstrated B. BB0619, DHH -DHHA1 domain p rotein (herein designated...

Vibrio vulnificus is an important zoonotic and aquatic pathogen can cause vibriosis in humans animals (especially farmed fish shrimp species). Rapid sensitive detection methods for V. are still required to diagnose human early reduce aquaculture losses. Herein, we developed a rapid diagnostic method comprising recombinase-aided amplification (RAA) assay the CRISPR/Cas12a system (named RAA-CRISPR/Cas12a) detect vulnificus. The RAA-CRISPR/Cas12a allows of 40 min without sophisticated...

Inflammation is an important risk factor in the development of inflammatory bowel disease (IBD) and colitis-associated colorectal cancer (CAC). Accumulating evidence indicates that some phytochemicals have anti-cancer properties. Polysaccharides extracted from Albuca bracteata (AB) been reported to possess anti-neoplastic activities on (CRC) models. However, it still unclear whether they exert therapeutic effects cancer. In this study, we investigate properties polysaccharides A. bracteate,...

T-cell anergy is a state of T cells that hyporesponsive to stimulation via the receptor and costimulatory molecules thought be important for self-tolerance. How regulated still poorly understood. We report here tuberous sclerosis (TSC)1 critical anergy. Deficiency TSC1 resulted in enhanced proliferation cytokine production absence cluster differentiation (CD)28-mediated costimulation, accompanied by metabolism. Resistance TSC1-deficient correlated with increased signaling through mammalian...

Abstract At present, diagnosis for neurosyphilis remains a major clinical challenge. Venereal Disease Research Laboratory (VDRL) titer of the cerebrospinal fluid (CSF) is suboptimally sensitive to diagnose neurosyphilis, which can be negative in patients, especially asymptomatic patients. In search biomarkers we investigated chemokine profile CSF patients and found that concentrations CXCL13, CXCL10 CXCL8 were selectively elevated correlated with protein concentration CSF-VDRL titer. After...

Fucoidan has many biological activities, including the inhibitory effect on development of various cancer types. This study showed that lipopolysaccharide-induced inflammation in FHC cells (normal human colonic epithelial cells) could be reversed using fucoidan at different concentrations. The fucoidan-induced anti-inflammatory was also confirmed through

Glycerol utilization as a carbohydrate source by

As an obligate pathogen, the Lyme disease spirochete Borrelia burgdorferi has a streamlined genome that encodes only two two-component signal transduction systems, Hk1-Rrp1 and Hk2-Rrp2 (in addition to CheA-CheY systems). The output of is production second messenger cyclic di-GMP (c-di-GMP), which indispensable for B. survive in tick vector. transcriptional activation global regulator RpoS, essential pathogen accomplish its tick-mouse transmission establish mammalian infection. Although...

Borrelia burgdorferi, the causative agent of Lyme disease, must adapt to two diverse niches, an arthropod vector and a mammalian host. RpoS, alternative sigma factor, plays central role in spirochetal adaptation host by governing expression many genes important for infection. B. burgdorferi is known be unique metal utilization, little biologically available metals burgdorferi. Here, we identified transition ions, manganese (Mn(2+)) zinc (Zn(2+)), that influenced regulation RpoS. The...

Background Human adenoviruses (HAdVs) have been recognised as pathogens that cause a broad spectrum of diseases. The studies on HAdV infection among children with severe acute respiratory (SARI) are limited. Objective To investigate the prevalence, epidemiology, and genotype SARI in China. Study Design Nasopharyngeal aspirates (NPAs) or induced sputum (IS) was collected from hospitalised SARIs Beijing (representing Northern China; n = 259) Zhejiang Province Eastern 293) 2007 to 2010....

High-temperature requirement protease A (HtrA) represents a family of serine proteases that play important roles in microbial biology. Unlike the genomes most organisms, Borrelia burgdorferi notably encodes single HtrA gene product, termed BbHtrA. Previous studies identified few substrates BbHtrA; however, their physiological relevance could not be ascertained, as targeted deletion has been successful. Here we show BbhtrA transcripts are induced during spirochete growth either stationary...

Corneal ulcers are a common eye inflammatory disease that can cause visual impairment or even blindness if not treated promptly. Ocular trauma is major risk factor for corneal ulcers, and in agricultural work rapidly progress to ulcers. This study aims evaluate the changes ocular surface (OS) microbiome of patients with traumatic ulcer (TCU).Among 20 healthy control (HC) subjects 22 TCU, 42 eyes were examined investigate OS microbial flora using metagenomic shotgun sequencing.At taxonomic...

Growing evidence supports the direct link of Fusobacterium nucleatum with colorectal cancer (CRC). However, to date, underlying mechanism action remains poorly understood. In this study, we examined effects F. on progression CRC and investigated whether cyclin-dependent kinase 5 (Cdk5) is involved in effect through activating Wnt/β-catenin signaling pathway.CRC tissues matched histologically normal specimens were collected from patients who diagnosed underwent surgical treatment our hospital...

The spread of antibiotic resistant bacteria (ARB) and resistance genes (ARGs) in hospital wastewater poses a great threat to public health, treatment plants (WWTPs) play an important role reducing the levels ARB ARGs. In this study, high-throughput metagenomic sequencing was used analyze bacterial community composition ARGs two hospitals exposed different use conditions (an eye specialty general hospital) before after treatment. results showed that there were various potential pathogenic...

A multimodal analytical strategy utilizing different modalities to cross-validate each other, can effectively minimize false positives or negatives and ensure the accuracy of detection results. Herein, we establish a colorimetric, photothermal, fluorescent triple modal CRISPR/Cas12a platform (CPF-CRISPR). An MNPs-ssDNA-HRP signal probe is designed act as substrate trigger three outputs. In presence DNA target, cleaved by activated CRISPR/Cas12a, resulting in release HRP generating short...

Current nucleic acid-responsive DNA hydrogels face significant challenges, such as the requirement for high target concentrations, frequent redesigns, and increased costs, which limit their practical applications in biosensing. To address these issues, we developed a novel biosensing platform integrating CRISPR/Cas12a system into an RCA-based hydrogel. The hydrogel used could preencapsulate diverse signal molecules comprising GelRed, methylene blue, gold nanoparticles, were released upon...