A5100706181- Wound Healing and Treatments
- Diabetic Foot Ulcer Assessment and Management
- Chronic Myeloid Leukemia Treatments
- Pressure Ulcer Prevention and Management
- Antimicrobial Peptides and Activities
- Spinal Cord Injury Research
- Enhanced Recovery After Surgery
- Electrospun Nanofibers in Biomedical Applications
- Cytokine Signaling Pathways and Interactions
- Periodontal Regeneration and Treatments
- Pharmacological Effects of Natural Compounds
- Protease and Inhibitor Mechanisms
- Chemokine receptors and signaling
- Acute Myeloid Leukemia Research
- Obstructive Sleep Apnea Research
- Venous Thromboembolism Diagnosis and Management
- Silk-based biomaterials and applications
- Computational Drug Discovery Methods
- High Altitude and Hypoxia
- Immunotherapy and Immune Responses
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Medicinal Plants and Bioactive Compounds
- NF-κB Signaling Pathways
- Advanced Glycation End Products research
- Natural Compounds in Disease Treatment
Capital Medical University
2024
Key Laboratory of Guangdong Province
2023-2024
Sun Yat-sen University
2014-2024
China Rehabilitation Research Center
2023-2024
Chinese Institute for Brain Research
2023-2024
The Seventh Affiliated Hospital of Sun Yat-sen University
2024
Beijing Shijitan Hospital
2024
Shenzhen University
2024
First Hospital of Jilin University
2023
Jilin University
2023
Diabetic wounds are recalcitrant to healing. However, the mechanism causing this dysfunction is not fully understood. High expression of matrix metalloproteinase-9 (MMP-9) indicative poor wound In study, we show that specificity protein-1 (Sp1), a regulator MMP-9, binds directly its promoter and enhances expression. Additionally, demonstrated Sp1 direct target two microRNAs (miRNAs), miR-129 -335, which significantly downregulated in diabetic skin tissues. vitro experiments confirmed or -335...
Qing-Luo-Yin (QLY) is a traditional Chinese medicine (TCM) formula used to treat Hot Syndrome-related rheumatoid arthritis (RA). Previously, we uncovered partial mechanisms involved in the therapeutic actions of QLY on RA. In this study, further elucidated its anti-rheumatic and investigated possible interactions with methotrexate (MTX) vivo using an integrating strategy coupled network pharmacology metabolomics techniques. Chemical composition was characterized by HPLC analysis. Collagen...
Abstract Excessive expression of matrix metalloproteinase 9 (MMP-9) impedes healing diabetic chronic wounds, thus wound dressing that could effectively inhibit the MMP-9 offers significant clinical translation for healing. Herein, a hybrid hydrogel was developed localized and sustained delivery siRNA (siMMP-9). siMMP-9 complexed with Gly-TETA (GT), GT/siMMP9 complex then loaded into thermosensitive based on Pluronic F-127 (PF) methylcellulose (MC). In vitro , this exhibited negligible...
Cancer stem cell behavior is thought to be largely determined by intrinsic properties and regulatory signals provided the microenvironment. Myelofibrosis (MF) characterized hematopoiesis occurring not only in marrow but also extramedullary sites such as spleen. In order study effects of these different microenvironments on primitive malignant hematopoietic cells, we phenotypically functionally splenic peripheral blood (PB) MF CD34+ cells from patients with MF. spleens contained greater...
For thousands of years, tonic herbs have been successfully used all around the world to improve health, energy, and vitality. However, their underlying mechanisms action in molecular/systems levels are still a mystery. In this work, two sets herbs, so called Qi-enriching (QEH) Blood-tonifying (BTH) TCM, were selected elucidate why they can restore proper balance harmony inside body, organ energy system. Firstly, pattern recognition model based on artificial neural network discriminant...
Background: Excessive expression of matrix metalloproteinase-9 (MMP-9) is deleterious to the cutaneous wound-healing process in context diabetes. The aim present study was explore whether a cationic star-shaped polymer consisting ß-cyclodextrin (ß-CD) core and poly(amidoamine) dendron arms (ß-CD-[D 3 ] 7 ) could be used as gene carrier small interfering RNA (siRNA) reduce MMP-9 for enhanced diabetic wound healing. Methods: cytotoxicity ß-CD-(D investigated by...
Abstract TNF receptor superfamily member 11a (TNFRSF11a, RANK) and its ligand 11 (TNFRSF11, RANKL) are overexpressed in many malignancies. However, the clinical importance of RANKL/RANK colorectal cancer (CRC) is mainly unknown. We examined CRC samples found that was elevated tissues compared with nearby normal tissues. A higher expression associated a worse survival rate. Furthermore, RANKL mostly produced by regulatory T cells (Tregs), which were able to promote advancement. Overexpression...
Background: We investigated the mechanism of delayed would healing caused by diabetes and measured dynamic changes in matrix metalloproteinase 9 (MMP-9) tissue inhibitor 1 (TIMP-1) levels. noted differences ratio MMP-9 to TIMP-1 wounds diabetic nondiabetic rats. Methods: Forty-two Sprague-Dawley rats weighing 250 g were randomly assigned either control group or streptozotocin-induced group. Then, full-thickness excision created on middle back each animal. Skin biopsy specimens obtained days...
Studies have documented that unusually high expression of matrix metalloproteinase-9 (MMP-9) suppresses wound healing during the late stages diabetic foot ulcers. Recently, it has been reported presence advanced glycation end products-bovine serum albumin (AGE-BSA) resulted in a higher MMP-9 skin primary keratinocytes. The aim present study was to elucidate molecular machinery is responsible for inappropriately AGE-BSA-induced MMP-9. It demonstrated site-specific DNA demethylation played an...
Abstract Impaired wound healing accompanies severe cell apoptosis in diabetic patients. Tissue inhibitor of metalloproteinases‐1 (TIMP‐1) was known to have effects on promoting growth and anti‐apoptosis for cells. We aimed determine the actual levels TIMP‐1 in: (i) biopsies non‐diabetic foot tissue (ii) human fibroblasts with or without treatments advanced glycation end‐products (AGEs). Next, we improved after either active protein vivo expression gene therapy vector‐mediated both animal...
Inappropriately high expression of matrix metalloproteinase 9 (MMP9) in the late stage diabetic foot ulcers suppresses wound healing. The underlying mechanisms are not completely understood. Site-specific demethylation was reported to function regulation genes, causing persistent target genes. Therefore, this study designed determine whether site-specific DNA a key regulatory component MMP9 healing, and further verify crucial CpG site(s). Human keratinocyte cell line (HaCaT) cells were...
Purpose: The purpose of this study was to assess the impact severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) on corneal stroma characteristics, ocular manifestations, and post-recovery refractive surgery outcomes after varying recovery durations. Methods: Fresh lenticules from patients with post-coronavirus disease 2019 (COVID-19; recovered within 135 days) healthy controls (HCs) small incision lenticule extraction (SMILE) were obtained for experimental validation SARS-CoV-2...
Traditional Chinese Medicine (TCM) doctors always prescribe various herbal formulae tailored to individual patients. However, there is still a lack of appropriate methods study the rule and potential biological basis underlying numerous prescriptions. Here we developed an Herb-Compound-Target-Disease coherent network approach analyze 871 prescriptions from TCM master, Mr. Ji-Ren Li, in his clinical practice on treatment rheumatoid arthritis (RA). The core herb networks were extracted Li's...
Dry eye syndrome (DES) is a prevalent postoperative complication after myopic corneal refractive surgeries and the main cause of dissatisfaction. Although great efforts have been made in recent decades, molecular mechanism DES remains poorly understood. Here, we used series bioinformatics approaches experimental methods to investigate potential involved DES.BALB/c mice were randomly divided into sham, unilateral nerve cutting (UCNV) + saline, UCNV vasoactive intestinal peptide (VIP),...
Polygonum perfoliatum L. is an herbal medicine that has been extensively used in traditional Chinese to treat various health conditions ranging from ancient internal surgical and gynecological diseases. Numerous studies suggest P. extract elicits significant anti-tumor, anti-inflammatory, anti-bacterial, anti-viral effects. Nevertheless, the underlying mechanisms of its anti-liver cancer effects remain poorly understood. Our study suggests stem (PPLA) a favorable safety profile exhibits...
Background: Matrix metalloproteinases (MMPs) degrade extracellular matrix components. Increased MMP-9 content in diabetic skin contributes to vulnerability and refractory foot ulcers. To identify ways decrease levels skin, inhibition of expression dermal fibroblasts using small interfering RNA was investigated vitro. Methods: A full-thickness wound created on the midback streptozotocin-induced rats; biopsies were performed 3 days later. Skin observed by immunohistochemical analysis. Dermal...
To observe and evaluate the short-term effectiveness of superior capsular reconstruction using autologous fascia lata graft for irreparable massive rotator cuff tears.The clinical data 9 patients with tears treated arthroscopic by between September 2019 April 2020 were retrospectively analysed. There 4 males 5 females a median age 66 years (range, 55-70 years). The disease duration was 6-60 months an average 19.1 months. According to Hamada classification, classified as grade 1 in 2 cases, 3...
<title>Abstract</title> TNF receptor superfamily member 11a (TNFRSF11a, RANK) and its ligand 11 (TNFRSF11, RANKL) are overexpressed in a number of malignancies. The clinical importance RANKL/RANK colorectal cancer (CRC) is, however, mainly unknown. We examined CRC patient samples found that was elevated tissues as compared to nearby normal tissues. A higher expression related with worse survival rate. Furthermore, we RANKL is mostly produced by regulatory T cells (Tregs), which can promote...