A5067742021- SARS-CoV-2 and COVID-19 Research
- Hepatitis C virus research
- COVID-19 Clinical Research Studies
- Respiratory viral infections research
- Animal Virus Infections Studies
- Hepatitis B Virus Studies
- Viral gastroenteritis research and epidemiology
- Mosquito-borne diseases and control
- Liver Disease Diagnosis and Treatment
- vaccines and immunoinformatics approaches
- Parvovirus B19 Infection Studies
- SARS-CoV-2 detection and testing
- T-cell and B-cell Immunology
- Viral Infections and Immunology Research
- Viral Infections and Vectors
- HIV Research and Treatment
- Immune Cell Function and Interaction
- Monoclonal and Polyclonal Antibodies Research
- Long-Term Effects of COVID-19
- interferon and immune responses
- Congenital Diaphragmatic Hernia Studies
- Tracheal and airway disorders
- Virus-based gene therapy research
- Diet, Metabolism, and Disease
- Inhalation and Respiratory Drug Delivery
University of South China
2016-2025
Chenzhou First People's Hospital
2016-2023
Southern Medical University
2018-2022
Xiangnan University
2022
Philadelphia VA Medical Center
2017
University of Pennsylvania
2017
Chinese Center For Disease Control and Prevention
2007-2016
Drexel University
2009-2013
National Institute for Viral Disease Control and Prevention
2007-2008
Center for Disease Control
2007
The 501Y.V2 variants of SARS-CoV-2 containing multiple mutations in spike are now dominant South Africa and rapidly spreading to other countries. Here, experiments with 18 pseudotyped viruses showed that the do not confer increased infectivity cell types except for murine ACE2-overexpressing cells, where a substantial increase was observed. Notably, susceptibility 12 17 neutralizing monoclonal antibodies substantially diminished, neutralization ability sera from convalescent patients...
The emergence of Omicron/BA.1 has brought new challenges to fight against SARS-CoV-2. A large number mutations in the Spike protein suggest that its susceptibility immune protection elicited by existing COVID-19 infection and vaccines may be altered. In this study, we constructed pseudotyped SARS-CoV-2 variant Omicron. sensitivity 28 serum samples from convalescent patients infected with original strain was tested Omicron as well other variants concern (VOCs, Alpha, Beta, Gamma, Delta)...
Coronavirus (CoV) infections induce respiratory tract illnesses and central nervous system (CNS) diseases. We aimed to explore the cytokine expression profiles in hospitalized children with CoV-CNS CoV-respiratory infections.A total of 183 236 acute encephalitis-like syndrome infection, respectively, were screened for anti-CoV IgM antibodies. The multiple cytokines determined CoV-positive patients.Anti-CoV antibodies detected 22/183 (12.02%) 26/236 (11.02%) patients respectively. Cytokine...
Coronavirus disease 2019 (COVID-19) is caused by infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)1–3 and individuals COVID-19 have symptoms that can be asymptomatic, mild, moderate or severe4,5. In the early phase of infection, T- B-cell counts are substantially decreased6,7; however, IgM8–11 IgG12–14 detectable within 14 d after symptom onset. COVID-19-convalescent individuals, spike-specific neutralizing antibodies variable3,15,16. No specific drug vaccine...
Circulating T follicular helper (cTfh) cells have been identified as counterparts of germinal center Tfh (GC Tfh) in humans and can support T-dependent B cell maturation antibody production vitro. However, the role cTfh neutralizing (nAb) responses HCV infection remains unclear. Here, we characterized phenotype function demonstrated associations their subsets with nAb infection. A total 38 HCV-infected individuals 28 healthy controls were enrolled from a pool injection drug users. The...
A newly identified parvovirus, human bocavirus (HBoV), was found in 21 (8.3%) of 252 nasopharyngeal aspirates from hospitalized children with lower respiratory tract infection Hunan Province, People's Republic China. Viral loads were 10(4) to 10(10) copies/mL. Phylogenetic analysis the VP1 gene showed a single genetic lineage HBoV worldwide.
ABSTRACT Imino sugars, such as N -butyl-deoxynojirimycin and -nonyl-deoxynojirimycin (NNDNJ), are glucose analogues that selectively inhibit cellular α-glucosidase I II in the endoplasmic reticulum exhibit antiviral activities against many types of enveloped viruses. Although these molecules have broad-spectrum activity, their development has been limited by a lack efficacy and/or selectivity. We previously reported DNJ derivative with hydroxylated cyclohexyl side chain, called OSL-95II, an...
Severe acute respiratory syndrome coronavirus 2 variants have continued to emerge in diverse geographic locations with a temporal distribution. The Lambda variant containing multiple mutations the spike protein, has thus far appeared mainly South America. harbours two receptor binding domain, L452Q and F490S, which may change its infectivity antigenicity neutralizing antibodies. In this study, we constructed 10 pseudoviruses study each individual amino acid mutation's effect on viral...
Long-term humoral immunity to SARS-CoV-2 is essential for preventing reinfection. The production of neutralizing antibody (nAb) and B cell differentiation are tightly regulated by T follicular help (T
Introduction Since December 2019, the emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) causing disease 2019 (COVID-19) has presented considerable public health challenges. Multiple vaccines have been used to induce neutralizing antibodies (nAbs) and memory B-cell responses against viral spike (S) glycoprotein, many essential epitopes defined. Previous reports identified spike-reactive naïve B cells preexisting in unexposed individuals. However, role these...
α-Glucosidases I and II are endoplasmic reticulum-resident enzymes that essential for N-linked glycan processing subsequent proper folding of glycoproteins. In this report, we first demonstrate downregulation the expression α-glucosidase I, II, or both in Huh7.5 cells by small hairpin RNA technology inhibited production hepatitis C virus (HCV). agreement with role α-glucosidases HCV envelope glycoprotein folding, treatment HCV-infected a panel imino sugar derivatives, which competitive...
We recently described the discovery of oxygenated N-alkyl deoxynojirimycin (DNJ) derivative 7 (CM-10-18) with antiviral activity against dengue virus (DENV) infection both in vitro and vivo. This imino sugar was promising but had an EC(50) DENV BHK cells 6.5 μM, which limited its use Compound presented structural opportunities for relationship analysis, we exploited report here. These structure-activity studies led to analogues 2h, 2l, 3j, 3l, 3v, 4b-4c nanomolar (EC(50) = 0.3-0.5 μM)...
Respiratory syncytial virus (RSV) infection is the leading cause of acute respiratory tract disease in children less than 5 years old. The aim this study was to further elucidate molecular properties and clinical characteristics RSV infection. sample included 238 patients <5 old who were hospitalized with symptoms upper or lower (URTI LRTI) Pediatric Department at First People's Hospital Chenzhou, South China 2014. We subjected nasopharyngeal aspirate (NPA) nasal swab (NS) samples from...
To determine whether the neutralization activity of monoclonal antibodies, convalescent sera and vaccine-elicited was affected by top five epidemic SARS-CoV-2 variants in UK, including D614G+L18F+A222V, D614G+A222V, D614G+S477N, VOC-202012/01(B.1.1.7) D614G+69-70del+N439K, a pseudovirus-neutralization assay performed to evaluate relative titers against 12 single deconvolution mutants based on variants. In this study, 18 10 from COVID-19 patients, inactivated-virus sera, 14 mRNA nine...
The outbreaks of severe acute respiratory syndrome coronavirus (SARS-CoV-1), Middle East (MERS-CoV), and SARS-CoV-2 highlight the need for countermeasures to prevent future pandemics. Given unpredictable nature spillover events, preparing antibodies with broad coronavirus-neutralizing activity is an ideal proactive strategy. Here, we investigated whether infection vaccination could provide cross-neutralizing (nAbs) against zoonotic sarbecoviruses. We evaluated profiles plasma monoclonal...
Emerging SARS-CoV-2 variants are the most serious problem for COVID-19 prophylaxis and treatment. To determine whether vaccine strain should be updated following variant emergence like seasonal flu vaccine, changed degree on antigenicity of H3N2 strains was compared. The neutralization activities Alpha, Beta Gamma variants' spike protein-immunized sera were analysed against eight current epidemic 20 possible combining top 10 prevalent RBD mutations based Delta variant, which constructed...
Abstract Hepatitis B virus/hepatitis C virus (HBV/HCV) dual infection is common among high-risk individuals. To characterize the virological and immunological features of patients with HBV/HCV infection, we enrolled 1,049 individuals who have been identified as injection drug users. Patients were divided into single groups according to serological markers. We found average HCV RNA level was significantly lower; however, HBV viral load higher in dual-infected (n = 42) comparing 340) or 136)....
Hepatitis B, C, and D virus (HBV, HCV, HDV) infections are known to be prevalent in injection drug users (IDUs); however, the relationship between molecular epidemiologic features of hepatitis infection high-risk individuals general population has not yet been established.In total, 1049 IDUs 672 who underwent physical examinations at Chenzhou hospital, Hunan Province, China, were enrolled. HBV, HDV screened with serologic tests both populations. HBsAg-positive, anti-HCV IgG-positive,...
Abstract A newly identified parvovirus, human bocavirus (HBoV), was found in 21 (8.3%) of 252 nasopharyngeal aspirates from hospitalized children with lower respiratory tract infection Hunan Province, People’s Republic China. Viral loads were 104 to 1010 copies/mL. Phylogenetic analysis the VP1 gene showed a single genetic lineage HBoV worldwide.