A5041693287- Hepatitis B Virus Studies
- Hepatitis C virus research
- Liver Disease Diagnosis and Treatment
- Glycosylation and Glycoproteins Research
- Herpesvirus Infections and Treatments
- Viral gastroenteritis research and epidemiology
- Monoclonal and Polyclonal Antibodies Research
- Hepatitis Viruses Studies and Epidemiology
- Virus-based gene therapy research
- Viral Infections and Outbreaks Research
- Galectins and Cancer Biology
- Cancer Genomics and Diagnostics
- Mosquito-borne diseases and control
- Hepatocellular Carcinoma Treatment and Prognosis
- HIV Research and Treatment
- Endoplasmic Reticulum Stress and Disease
- interferon and immune responses
- Viral Infections and Vectors
- Carbohydrate Chemistry and Synthesis
- Poxvirus research and outbreaks
- Toxin Mechanisms and Immunotoxins
- Cytomegalovirus and herpesvirus research
- Animal Virus Infections Studies
- Bacteriophages and microbial interactions
- Pancreatic and Hepatic Oncology Research
Baruch S. Blumberg Institute
2015-2025
Drexel University
2008-2024
Hepatitis B Foundation
2013-2023
Bucks County Division of Human Services
2021
Medical University of South Carolina
2017
Stanford University
2006-2016
Fox Chase Cancer Center
1996-2015
Alaska Native Tribal Health Consortium
2015
University of Toronto
2015
Doylestown Hospital
2005-2014
Chronic infection with hepatitis B virus (HBV) is associated the majority of hepatocellular carcinoma (HCC). The diagnosis HCC usually made in late stages disease, when treatment options are limited and prognosis poor. We therefore have developed a method glycoproteomic analysis an attempt to discover serum markers that can assist early detection HBV-induced liver cancer. Briefly, comparative for oligosaccharides released from glycoproteins recovery identification proteins aberrant...
ABSTRACT Interferons (IFNs) are key mediators of the host innate antiviral immune response. To identify IFN-stimulated genes (ISGs) that instigate an state against two medically important flaviviruses, West Nile virus (WNV) and dengue (DENV), we tested 36 ISGs commonly induced by IFN-α for activity viruses. We discovered five efficiently suppressed WNV and/or DENV infection when they were individually expressed in HEK293 cells. Mechanistic analyses revealed structurally related cell plasma...
Covalently closed circular DNA (cccDNA) of hepatitis B virus (HBV) is formed by conversion capsid-associated relaxed (rcDNA) via unknown mechanisms and exists in the nucleus infected hepatocyte as a minichromosome that serves transcription template for viral RNAs. To study molecular pathway cccDNA formation its regulation cellular factors, we have established cell line supports replication an envelope protein-deficient HBV genome tetracycline-inducible manner. Following induction...
ABSTRACT Tetherin and IFITM3 are recently identified interferon-induced cellular proteins that restrict infections by retroviruses filoviruses of influenza virus flaviviruses, respectively. In our efforts to further explore their antiviral activities against other viruses determine mechanisms, we found the two potently inhibit infection vesicular stomatitis (VSV), a prototype member Rhabdoviridae family. Taking advantage this well-studied system, show although both tetherin plasma membrane...
The zinc finger antiviral protein (ZAP) is a mammalian host restriction factor that inhibits the replication of variety RNA viruses, including retroviruses, alphaviruses and filoviruses, through interaction with ZAP-responsive elements (ZRE) in viral RNA, recruiting exosome to degrade substrate. Hepatitis B virus (HBV) pararetrovirus replicates its genomic DNA via reverse transcription pregenomic (pg) precursor. Here, we demonstrate two isoforms human ZAP (hZAP-L -S) inhibit HBV...
IFNs are a family of cytokines that essential for the antiviral response in vertebrates. Not surprisingly, viruses have adapted to encode virulence factors cope with IFN response. Intriguingly, we show here all three types interferons, IFN-α, IFN-γ, and IFN-λ, efficiently promote infection by human coronavirus, HCoV-OC43, one major etiological agents common cold, through induction IFN-inducible transmembrane (IFITM) proteins. IFITMs typically exert their function inhibiting entry broad...
One function of N-linked glycans is to assist in the folding glycoproteins by mediating interactions lectin-like chaperone proteins calnexin and calreticulin with nascent glycoproteins. These can be prevented inhibitors α-glucosidases, such as N -butyl-deoxynojirimycin (NB-DNJ) -nonyl-DNJ (NN-DNJ), this causes some misfolded retained within endoplasmic reticulum (ER). We have shown previously that NN-DNJ-induced misfolding one hepatitis B virus (HBV) envelope prevents formation secretion...
ABSTRACT Hepatitis C virus (HCV) infection is a common cause of chronic hepatitis and currently treated with alpha interferon (IFN-α)-based therapies. However, the underlying mechanism IFN-α therapy remains to be elucidated. To identify cellular proteins that mediate antiviral effects IFN-α, we created HEK293-based cell culture system inducibly express individual interferon-stimulated genes (ISGs) determined their against HCV. By screening 29 ISGs are induced in Huh7 cells by and/or...
Changes in N-linked glycosylation are known to occur during the development of cancer. For example, increased branching oligosaccharides has been associated with metastasis and correlated tumor progression human cancers breast, colon melanomas. Increases core fucosylation have also hepatocellular carcinoma (HCC). Chronic infection hepatitis B virus is more than 55% all cases carcinoma. We show here that levels can be observed via glycan analysis total serum HCC. In a blinded study,...
The liver-specific microRNA miR-122 has been shown to be required for the replication of hepatitis C virus (HCV) in hepatoma cell line Huh7. aim this study was test if HCV can modulated by exogenously expressed human embryonic kidney epithelial cells (HEK-293). Our results demonstrate that enhances colony formation efficiency replicon and increases steady-state level RNA HEK-293 cells. Therefore, we conclude although is not absolutely required, it greatly nonhepatic
ABSTRACT Hepatitis B virus (HBV) covalently closed circular DNA (cccDNA) plays a central role in viral infection and persistence is the basis for rebound after cessation of therapy, as well elusiveness cure even extended treatment. Therefore, there an urgent need development novel therapeutic agents that directly target cccDNA formation maintenance. By employing innovative cell-based assay which secreted HBV e antigen cccDNA-dependent surrogate, we screened in-house small-molecule library...
Patient serum or plasma is frequently monitored for biochemical markers of disease physiological status. Many the rapidly evolving technologies proteome analysis are being used to find additional clinically informative protein markers. The unusually high abundance albumin in can interfere with resolution and sensitivity many profiling techniques. We have monoclonal antibodies against human (HSA) develop an immunoaffinity resin that effective removal both full-length HSA fragments present...
The imino sugar N-butyldeoxynojirimycin (NBDNJ) is a potent inhibitor of the oligosaccharide-trimming enzyme alpha-glucosidase I. Hepatitis B virus (HBV) contains three surface proteins (HBs proteins) different sizes that are singly or doubly N-glycosylated and essential for formation infectious virus. Therefore, replication secretion HBV in human hepatoma cell line HepG2 were studied presence NBDNJ. In stably HBV-transfected HepG 2.2.15 cells HBV-infected cells, NBDNJ suppressed particles...
ABSTRACT Chronic hepatitis B virus (HBV) infection, a serious public health problem leading to cirrhosis and hepatocellular carcinoma, is currently treated with either pegylated alpha interferon (pegIFN-α) or one of the five nucleos(t)ide analogue viral DNA polymerase inhibitors. However, neither pegIFN-α nor analogues are capable reliably curing infection. In order develop novel antiviral drugs against HBV, we established cell-based screening assay by using an immortalized mouse...