A5075901638- Hepatitis B Virus Studies
- Hepatitis C virus research
- Liver Disease Diagnosis and Treatment
- Hepatitis Viruses Studies and Epidemiology
- Viral gastroenteritis research and epidemiology
- Vaccine Coverage and Hesitancy
- Bacteriophages and microbial interactions
- MicroRNA in disease regulation
- Endoplasmic Reticulum Stress and Disease
- Viral Infections and Outbreaks Research
- Vibrio bacteria research studies
- RNA Interference and Gene Delivery
- Genomics and Phylogenetic Studies
- Respiratory viral infections research
- COVID-19 Clinical Research Studies
- HIV, Drug Use, Sexual Risk
- Immunotherapy and Immune Responses
- Zoonotic diseases and public health
- HIV/AIDS Research and Interventions
- HIV Research and Treatment
- COVID-19 Impact on Reproduction
- Chronic Kidney Disease and Diabetes
- Virus-based gene therapy research
- Literature Analysis and Criticism
- Liver Disease and Transplantation
University of the Witwatersrand
2016-2025
National and Kapodistrian University of Athens
2024
Groote Schuur Hospital
2013
National Institutes of Health
2013
South African National Blood Service
2011
Cancer Association of South Africa
1999-2010
University of KwaZulu-Natal
1999-2002
South African Medical Research Council
2000-2001
The University of Tokyo
2001
Of approximately 360 million people in the world chronically infected with hepatitis B virus (HBV), 65 reside Africa. Thus, Africa, 12% of world's population, carries 18% global burden HBV infection, hepatocellular carcinoma and cirrhosis accounting for 2% continent's annual deaths. Despite being endemic or hyperendemic there is a paucity data on genotypes their distribution. Genotype A found mainly southern, eastern central Most African genotype strains belong to subgenotype A1, A3 western...
Various genotypes of the hepatitis B virus (HBV) induce liver disease distinct severity, but underlying virological differences are not well defined. Huh7 cells were transfected with plasmids carrying 1.24-fold HBV genome different genotypes/subgenotypes (2 strains each for Aa/A1, Ae/A2, Ba/B2 and D; 3 Bj/B1 C). DNA levels in cell lysates, determined by Southern hybridization, highest C followed Bj/Ba D/Ae (P < .01), lowest Aa whereas culture media, they Bj, distantly Ba/C/D further Ae/Aa...
The purpose of this study was to identify mutations in the basic core promoter and enhancer II region hepatitis B virus (HBV) that might cause HBV e antigen (HBeAg)-negative phenotype contribute hepatocarcinogenesis black African carriers virus. promoter/enhancer overlaps with X gene. DNA from serum 47 asymptomatic 50 patients hepatocellular carcinoma 28 tumor 10 nontumor liver tissues amplified sequenced directly. That part not overlapping completely conserved all samples. Missense at...
Abstract Using phylogenetic analysis and pairwise comparison of 670 complete hepatitis B virus (HBV) genomes, we demonstrated that nucleotide divergence greater than 7.5% can be used to separate strains into genotypes A–H. Strains separated subgenotypes when two criteria are met: about 4% but less good bootstrap support. There is a highly statistically significant association between serological subtypes ( χ 2 ‐test for association, P < 0.0001): adw associated with A, B, F, G, H, adr C...
Abstract Genotypes A, D, and E of the hepatitis B virus (HBV) circulate in southern Africa, with genotype A predominating. Their hepatocarcinogenic potential Bantu‐speaking sub‐saharan Africans is, however, unknown. Using a case/control format, we investigated these genotypes subgenotype A1 which accounts for great majority isolates. HBV isolates from 111 unselected hepatocellular carcinoma (HCC) matched asymptomatic chronic carriers, serving as controls, were genotyped using method [Lindh...
There are two subtypes of hepatitis B virus genotype A (HBV/A) and they provisionally designated Aa (“a” standing for Africa/Asia) Ae (“e” Europe). In a case-control study, 78 HBV/Aa, 78HBV/Ae, 78HBV/D carriers from several countries were compared. The prevalence HBe antigen (HBeAg) in serum was significantly lower HBV/Aa than HBV/Ae (31% vs. 49%; P = .033), with difference more obvious the aged 30 years or younger (34% 67%; .029). HBV DNA levels (median, 3.46 log copies/mL; 95% CI,...
Phylogenetic analysis of hepatitis B virus (HBV) has led to its classification into eight genotypes, A H. The dominant genotype in South Africa is A, which consists two subgenotypes, A1 and A2. Subgenotype (previously subgroup A') predominates over subgenotype A2 minus A'). complete genome HBV isolated from 18 asymptomatic carriers the five acute patients was amplified; resulting amplicons were cloned sequenced. All isolates belonged had no distinguishing mutations relative carriers, a...
A phylogenetic analysis is presented of six complete and seven pre-S1/S2/S gene sequences hepatitis B virus (HBV) isolates from South Africa. Five the full-length all pre-S2/S have been previously reported. Four genomes three five incomplete clustered with subgroup A′, a unique segment genotype HBV identified in 60% African using region alone. This separation was also evident when polymerase open reading frame analysed, but not on either X or pre-core/core genes. Amino acids were pre-S1...
Since October 2005, a total of 2,921,561 blood donations have been screened by the South African National Blood Service for hepatitis B virus (HBV) individual-donation nucleic acid testing (ID-NAT). Over 4 years, 149 surface antigen-negative acute-phase HBV NAT-positive were identified (1:19,608). The lookback program one probable transmission.The complete genomes isolated from donor and recipient sequenced, cloned, analyzed phylogenetically. window period (WP) transmission risk was...
The aim of the present study was to systematically and comparatively analyze subgenotypes genotype D hepatitis B virus.In total, 304 complete genomes all were downloaded from public databases. sequences analyzed using nucleotide divergence calculations, phylogenetic analysis bioinformatics detect amino acids signature motifs for each subgenotype define their geographical distribution.Intragroup ranged 0.8 ± 0.5 (% standard deviation) D6 3.0 0.3 D8. Inter-subgenotype mostly 4-7.5%....
Abstract The 69th World Health Assembly endorsed the Global Sector Strategy for Viral Hepatitis, embracing a goal to eliminate hepatitis infection as public health threat by 2030. This was followed Organization's (WHO) global targets care and management of B virus (HBV) C (HCV) infections. These announcements were important in raising awareness calling action; however, tracking countries’ progress towards these elimination goals has provided insights limitations targets. existing compare...