A5080651363- Peptidase Inhibition and Analysis
- Hepatitis B Virus Studies
- Hepatitis C virus research
- Cancer Mechanisms and Therapy
- interferon and immune responses
- Immune cells in cancer
- Mosquito-borne diseases and control
- Viral Infections and Vectors
- Viral gastroenteritis research and epidemiology
- Cancer Immunotherapy and Biomarkers
- Immune Cell Function and Interaction
- Breast Cancer Treatment Studies
- Ubiquitin and proteasome pathways
- Autophagy in Disease and Therapy
- Ultrasound and Hyperthermia Applications
- Bone health and treatments
- Cancer-related molecular mechanisms research
- Epigenetics and DNA Methylation
- Genetic factors in colorectal cancer
- Cell death mechanisms and regulation
- Cancer Treatment and Pharmacology
- Chronic Lymphocytic Leukemia Research
- Circular RNAs in diseases
- Liver Disease Diagnosis and Treatment
- Immune Response and Inflammation
Hubei Cancer Hospital
2016-2025
Huazhong University of Science and Technology
2017-2025
Shanghai Jiao Tong University
2015-2024
Tiande (China)
2024
Second Affiliated Hospital of Jilin University
2021-2024
Jilin University
2014-2024
First Affiliated Hospital of Xinxiang Medical University
2014-2024
First Hospital of Jilin University
2014-2024
International Peace Maternity & Child Health Hospital
2024
Shanghai University of Medicine and Health Sciences
2021-2022
IFNs are a family of cytokines that essential for the antiviral response in vertebrates. Not surprisingly, viruses have adapted to encode virulence factors cope with IFN response. Intriguingly, we show here all three types interferons, IFN-α, IFN-γ, and IFN-λ, efficiently promote infection by human coronavirus, HCoV-OC43, one major etiological agents common cold, through induction IFN-inducible transmembrane (IFITM) proteins. IFITMs typically exert their function inhibiting entry broad...
Covalently closed circular DNA (cccDNA) of hepadnaviruses exists as an episomal minichromosome in the nucleus infected hepatocyte and serves transcriptional template for viral mRNA synthesis. Elimination cccDNA is prerequisite either a therapeutic cure or immunological resolution HBV infection. Although accumulating evidence suggests that inflammatory cytokines-mediated virally hepatocytes does occur plays essential role acute infection, molecular mechanism by which cytokines eliminate...
ABSTRACT Interferon-induced transmembrane proteins (IFITMs) are restriction factors that inhibit the infectious entry of many enveloped RNA viruses. However, we demonstrated previously human IFITM2 and IFITM3 essential host facilitating coronavirus (HCoV) OC43. In a continuing effort to decipher molecular mechanism underlying IFITM differential modulation HCoV entry, investigated roles structural motifs important for protein posttranslational modifications, intracellular trafficking,...
The herpes simplex virus 2 (HSV-2) is one of the most important sexually transmitted pathogens, and can facilitate spread human immunodeficiency virus.The currently available antiviral drugs have certain limitations.Nanosilver has received increasing attention recently with respect to its antibacterial properties.The purpose this study was determine inhibiting effect mechanism silver nanoparticles (Ag-NPs) on HSV-2.The cytotoxicity Vero cells induced by different Ag-NP concentrations...
Hepatitis B virus (HBV) core protein assembles viral pre-genomic (pg) RNA and DNA polymerase into nucleocapsids for reverse transcriptional replication to take place. Several chemotypes of small molecules, including heteroaryldihydropyrimidines (HAPs) sulfamoylbenzamides (SBAs), have been discovered allosterically modulate structure consequentially alter the kinetics pathway assembly, resulting in formation irregularly-shaped aggregates or "empty" capsids devoid polymerase. Interestingly,...
Abstract Mutations in KRAS exon 2, BRAF and PIK3CA are commonly present colorectal cancer (CRC) worldwide, but few data about RAS mutations outside 2 available for Chinese CRCs. We, therefore, determined the mutation frequencies prognostic values of 3 4, NRAS 3, 9 20, 15 by PCR direct sequencing 353 CRC patients from two clinical centers. , were identified 42.2%, 4.5%, 12.3% cases, respectively. We found “rare mutations” genes nearly 14% CRCs-i.e., almost a quarter (24.0%) wild type CRCs,...
BackgroundAsparaginyl endopeptidase (AEP) has been implicated in human cancer development. However, the molecular mechanisms underlying AEP regulation, including role of pro-AEP activation, remain elusive.
// Jiu-Ming Zou 1, * , Jian Qin Yong-Chao Li 1 Yu Wang Dong Shu Chao Luo Shan-Shan Gang Chi Fang Guo Gui-Mei Zhang Zuo-Hua Feng Department of Biochemistry and Molecular Biology, School Basic Medicine, Tongji Medical College, Huazhong University Science Technology, Wuhan 430030, The People’s Republic China These authors have contributed equally to this work Correspondence to: Feng, email: fengzhg_tj@163.com Li, yongchaolee@outlook.com Keywords: IL-35, neutrophils, inflammation, tumor,...
To predict the American Joint Cancer Committee tumor-node-metastasis stage in patients with papillary thyroid carcinoma by evaluating relationship between preoperative neutrophil-to-lymphocyte ratio and stage.We retrospectively examined 161 a diagnosis of carcinoma. The Neutrophil-to-Lymphocyte Ratio was calculated according to absolute neutrophil counts lymphocyte on routine blood tests obtained prior surgery 2.0 or more were classified as high NLR group, while those less than low group....
Endoplasmic reticulum (ER)-resident glucosidases I and II sequentially trim the three terminal glucose moieties on N-linked glycans attached to nascent glycoproteins. These reactions are first steps of glycan processing essential for proper folding function many Because most viral envelope glycoproteins contain glycans, inhibition ER with derivatives 1-deoxynojirimycin, i.e., iminosugars, efficiently disrupts morphogenesis a broad spectrum enveloped viruses. However, like proteins, cellular...
The STAT3 transcription factor is a major intracellular signaling protein and frequently dysregulated in the most common lethal brain malignancy adults, glioblastoma multiforme (GBM). Activation of GBM correlates with poor prognosis. phosphorylating signal transducer JAK2 activates response to cytokines growth factors. Currently there are no JAK-STAT pathway inhibitors clinical trials for GBM, so we sought examine anti-GBM activity SAR317461 (Sanofi-Aventis), newer generation, highly potent...
ABSTRACT Induction of interferon and proinflammatory cytokines is a hallmark the infection many different viruses. However, hepatitis B virus (HBV) does not elicit detectable cytokine response in infected hepatocytes. In order to investigate molecular mechanism underlying innate immune evasion, functional cyclic GMP-AMP (cGAMP) synthase (cGAS)-stimulator genes (STING) pathway was reconstituted human hepatoma cell line supporting tetracycline-inducible HBV replication. It demonstrated that...
Sperm motility is the main index used to assess quality of bull semen. It may also be evaluate fertility potential bulls. Protein-coding mRNA and long noncoding RNA (lncRNA) participate in regulation spermatogenesis. Here, we employed strand-specific sequencing profile semen transcriptome (mRNA lncRNA) six paired full-sibling Holstein bulls with divergent sperm determine functions lncRNA motility. Among 20,875 protein-encoding genes detected semen, 19 were differentially expressed between...
Tumor‐associated macrophages ( TAM ) play a critical role in promoting tumor development and metastasis. In the present study, we found that legumain, an asparaginyl endopeptidase, was highly expressed on surface of . A doxorubicin‐based prodrug specifically activated by legumain selectively ablated resulted significant reduction angiogenic factors related vessel growth. Treatment with also suppressed circulating cells myeloid immune suppressor Gr‐1+/CD11b+ tumor‐bearing animals. After...
Background The Macleaya spp., including cordata and microcarpa, are traditional anti-virus, inflammation eliminating, insecticide herb medicines for their isoquinoline alkaloids. They also known as the basis of popular natural animal food addictive in Europe. However, few studies especially at genomics level were conducted on them. Hence, we performed spp. transcriptome integrated it with iTRAQ proteome analysis order to identify potential genes involved alkaloids biosynthesis. Methodology...
Mutations in KRAS, BRAF and PIK3CA are the most common somatic alterations found colorectal cancer (CRC) patients from Western countries; but their prevalence prognostic value have not been adequately assessed Asian patients. The aim of this study was to determine mutation frequencies these genes Chinese CRC investigate impact on prognosis. sequences exon 2 15 exons 9 20 were evaluated by PCR direct sequencing using DNA extracted formalin-fixed paraffin-embedded (FFPE) tissues primary tumors...
Infection by Zika virus (ZIKV) is linked to microcephaly and other neurological disorders, posing a significant health threat. Innate immunity the first line of defense against invading pathogens, but relatively little understood regarding host intrinsic mechanisms that guard ZIKV. Here, we show tripartite motif-containing protein 56 (TRIM56) poses barrier ZIKV infection in cells neural, epithelial fibroblast origins. Overexpression TRIM56, not an E3 ligase-dead mutant or one lacking short...
ABSTRACT Although a highly effective vaccine is available, the number of yellow fever cases has increased over past 2 decades, which highlights pressing need for antiviral therapeutics. In high-throughput screening campaign, we identified an acetic acid benzodiazepine (BDAA) compound potently inhibits virus (YFV). Interestingly, while treatment YFV-infected cultures with μM BDAA reduced virion production by greater than logs, was not active against 21 other viruses from 14 different viral...
Significance BRUCE/Apollon is an unusual inhibitor of apoptosis protein containing a ubiquitin-conjugating domain. Here we show that BRUCE also autophagy by promoting the proteasomal degradation LC3-I, precursor LC3-II, which critical in autophagosome biogenesis and substrate recruitment. SIP/CacyBP, highly expressed neurons various tumors, promotes enhancing autophagic formation. DNA damage anticancer agents camptathecin etoposide caused monoubiquitination SIP ubiquitin ligase Nrdp1...
Salinomycin is a well-known inhibitor of human cancer stem cells (CSCs). However, the molecular mechanism(s) by which salinomycin targets colorectal CSCs poorly understood. Here, we have investigated underlying antitumour mechanisms in and three tumour models.