A5022547903- interferon and immune responses
- Hepatitis C virus research
- Hepatitis B Virus Studies
- MicroRNA in disease regulation
- Galectins and Cancer Biology
- Cytokine Signaling Pathways and Interactions
- SARS-CoV-2 and COVID-19 Research
- Inflammatory mediators and NSAID effects
- RNA Research and Splicing
- Cellular transport and secretion
- RNA Interference and Gene Delivery
- Viral Infections and Vectors
- Immune Response and Inflammation
- Graphene and Nanomaterials Applications
- RNA regulation and disease
- Virus-based gene therapy research
- Cancer Mechanisms and Therapy
- Renal Diseases and Glomerulopathies
- Glycosylation and Glycoproteins Research
- Systemic Lupus Erythematosus Research
- Inflammasome and immune disorders
- Advanced biosensing and bioanalysis techniques
- Virology and Viral Diseases
- Atherosclerosis and Cardiovascular Diseases
- Phagocytosis and Immune Regulation
University of Tennessee Health Science Center
2017-2023
Central South University
2013-2023
Le Bonheur Children's Hospital
2019-2023
Second Xiangya Hospital of Central South University
2023
Hunan Provincial Center for Disease Control and Prevention
2023
State Key Laboratory of Chemobiosensing and Chemometrics
2013-2019
Hunan University
2011-2019
Hunan Cancer Hospital
2013-2019
University of South China
2015
National Institutes of Health
1995
Nanographene oxide (NGO) are highly suitable to be the shells of inorganic nanomaterials enhance their biocompatibility and hydrophilicity for biomedical applications while retaining useful photonic, magnetic, or radiological functions. In this study, a novel nanostructure with gold nanorods (AuNRs) encapsulated in NGO is developed an ultraefficient chemophotothermal cancer therapy agent. The decrease toxicity surfactant-coated AuNRs provide anchor points conjugation hyaluronic acid (HA)....
Significance More than 500 million people are persistently infected with hepatitis B virus (HBV) and/or C (HCV) and at a risk of developing chronic hepatitis, cirrhosis, liver cancer. The absence robust cell culture systems for both viral infections limits the understanding lifecycle pathogenesis required development vaccine antivirals. We have established novel human hepatoma line termed “HLCZ01” that supports entire HBV HCV produced in clinically. This provides powerful tool addressing...
Surface modification of inorganic nanoparticles (NPs) is extremely necessary for biomedical applications. However, the processes conjugating ligands to NPs surface are complicated with low yield. In this study, a hydrophilic shell excellent biocompatibility was successfully constructed on individual gold or nanorods (NRs) by encapsulating NRs in graphene oxide (GO) nanosheets through electrostatic self-assembly. This versatile and facile approach remarkably decreased cytotoxicity capping...
ABSTRACT Activation of innate immunity is essential for host cells to restrict the spread invading viruses and other pathogens. However, attenuation or termination signaling also necessary preventing immune-mediated tissue damage spontaneous autoimmunity. Here, we identify nucleotide binding oligomerization domain (NOD)-like receptor X1 (NLRX1) as a negative regulator mitochondrial antiviral protein (MAVS)-mediated pathway during hepatitis C virus (HCV) infection. The depletion NLRX1...
Interferons (IFNs) restrict various kinds of viral infection via induction hundreds IFN-stimulated genes (ISGs), while the functions majority ISGs are broadly unclear. Here, we show that a high-IFN-inducible gene, ISG12a (also known as IFI27), exhibits nonapoptotic antiviral effect on hepatitis C virus (HCV) infection. Viral NS5A protein is targeted specifically by ISG12a, which mediates degradation ubiquitination-dependent proteasomal pathway. K374R mutation in domain III abrogates...
Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is an attractive death in targeted cancer therapy. Many cells are refractory to TRAIL-induced cell and the mechanisms underlying resistance unclear. The molecular of HCC gastric resistant apoptosis were explored using biological immunological methods. In vivo experiments conducted study effect interferon stimulated gene 12a (ISG12a) on human liver xenografts mice. ISG12a decreases TRAIL-resistant cells. regulates sensitivity...
Infection by Zika virus (ZIKV) is linked to microcephaly and other neurological disorders, posing a significant health threat. Innate immunity the first line of defense against invading pathogens, but relatively little understood regarding host intrinsic mechanisms that guard ZIKV. Here, we show tripartite motif-containing protein 56 (TRIM56) poses barrier ZIKV infection in cells neural, epithelial fibroblast origins. Overexpression TRIM56, not an E3 ligase-dead mutant or one lacking short...
Hepatitis C virus (HCV) core protein is essential for assembly. HCV was expressed and purified. Aptamers against were raised through the selective evolution of ligands by exponential enrichment approach. Detection infection aptamers antiviral activities characterized. The mechanism their anti-HCV activity determined. data showed that selected specifically recognize recombinant but also can detect serum samples from hepatitis patients. have no effect on RNA replication in infectious cell...
High-mobility group box 1 (HMGB1) protein is a highly conserved nuclear involved in multiple human diseases, including infectious immune disorders, metabolic and cancer. HMGB1 comprised of two tandem HMG boxes (the A the B box) containing DNA-binding domains an acidic C-terminal peptide. It has been reported that enhances viral replication by binding to proteins. However, its role hepatitis C virus (HCV) unknown. Here, we show promoted HCV but had no effect on translation. RNA...
ABSTRACT Hepatitis C virus (HCV) envelope protein (E1E2) is essential for binding to host cells. Aptamers have been demonstrated strong promising applications in drug development. In the current study, a cDNA fragment encoding entire E1E2 gene of HCV was cloned. expressed and purified. were selected by method selective evolution ligands exponential enrichment (SELEX), antiviral actions aptamers examined. The mechanism their activity investigated. data show that specifically recognize...
It has been reported that IFN-λs inhibit HCV replication in vitro. But the mechanisms of how IL-28A conducts antiviral activity and functions IL-28A-induced ISGs (IFN-stimulated genes) are not fully understood. In this study, we found effect on life cycle including viral replication, assembly, release. IFN-α synergistically virus replication. EPSTI1 (epithelial-stromal interaction 1), one ISGs, plays a vital role IL-28A-mediated activity. Furthermore, forced expression effectively inhibits...
The interaction between hepatitis C virus (HCV) and human hepatic innate antiviral responses is unclear. aim of this study was to examine how hepatocytes respond HCV infection. An infectious isolate, JFH1, used infect a newly established hepatoma cell line HLCZ01. Viral RNA or NS5A protein examined by real-time PCR immunofluorescence respectively. mechanisms HCV-induced IFN-β apoptosis were explored. Our data showed that HLCZ01 cells supported the entire lifecycle interferon-stimulated genes...
NS2 protein is essential for hepatitis C virus (HCV) replication. was expressed and purified. Aptamers against were raised antiviral effects of the aptamers examined. The molecular mechanism through which exert their anti-HCV activity investigated. data showed that aptamer NS2-3 inhibited HCV RNA replication in replicon cell line infectious culture system. another NS2-2 demonstrated to inhibit production without cytotoxicity vitro. They did not affect B Interferon beta (IFN-β)...
Background and Aim The interaction between hepatitis C virus (HCV) innate antiviral defense systems in primary human hepatocytes is not well understood. objective of this study to examine how response HCV infection. Methods An infectious isolate JFH1 was used infect isolated hepatocytes. RNA or NS5A protein the cells detected by real-time PCR immunofluorescence staining respectively. Apoptosis examined with flow cytometry. Mechanisms HCV-induced IFN-β expression apoptosis were determined....
There are currently no effective therapies for COVID-19 or antivirals against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), and vaccines appear less new SARS-CoV-2 variants; thus, there is an urgent need to understand better the virulence mechanisms of host response develop therapeutic agents. Herein, we show that Neu1 regulates replication by controlling sialylation on nucleocapsid protein. Coronavirus proteins in patients HCoV-OC43-infected cells were heavily sialylated;...
The activation of interferon (IFN)-regulatory factor-3 (IRF3), characterized by phosphorylation and nuclear translocation the latent transcription factor, is central to initiating innate antiviral responses. Whereas much has been learned about upstream pathways signaling mechanisms leading IRF3 activation, how activated operates in nucleus control IFNs remains obscure. Here we identify EAP30 (a.k.a, SNF8/VPS22), an endosomal sorting complex required for transport (ESCRT)-II subunit, as...
Interferon-α (IFN-α) is an adjuvant to chemotherapy and radiotherapy for hepatocellular carcinoma (HCC), but some HCC patients do not respond treatment with IFN-α.We performed loss-of-function gain-of-function experiments examine the role of ISG15 in IFN-α sensitivity LH86, HLCZ01, SMMC7721, Huh7 cell lines tumor samples.The overexpression reduced apoptosis LH86 cells presence IFN-α, whereas shRNA-mediated knock down expression increased both cells. We identified a putative miR-370 target...
Hepatocellular carcinoma (HCC) is a leading cause of cancer-related mortality worldwide. Interferon-alpha (IFN-α) has recently been recognized to harbor therapeutic potential in the prevention and treatment HCC, but it remains controversial as whether IFN-α exerts direct cytotoxicity against HCC. Cyclooxygenase-2 (COX-2) overexpressed HCC considered play role hepatocarcinogenesis. Therefore, we aimed elucidate combined effect COX-2 inhibitor, celecoxib, on vitro growth suppression using...