A5065103299- SARS-CoV-2 and COVID-19 Research
- interferon and immune responses
- COVID-19 Clinical Research Studies
- Hepatitis B Virus Studies
- Hepatitis C virus research
- Viral gastroenteritis research and epidemiology
- Animal Virus Infections Studies
- Viral Infections and Vectors
- Viral Infections and Outbreaks Research
- Virus-based gene therapy research
- Plant Virus Research Studies
- Viral Infections and Immunology Research
- Long-Term Effects of COVID-19
- Mosquito-borne diseases and control
- SARS-CoV-2 detection and testing
- Evolution and Genetic Dynamics
- Plant and Fungal Interactions Research
- vaccines and immunoinformatics approaches
- RNA and protein synthesis mechanisms
- Influenza Virus Research Studies
- Phytochemistry and Bioactivity Studies
- CNS Lymphoma Diagnosis and Treatment
- COVID-19 diagnosis using AI
- HIV/AIDS drug development and treatment
- Plant Pathogens and Resistance
Beijing Ditan Hospital
1970-2025
Capital Medical University
1970-2025
National Marine Environmental Forecasting Center
1970-2023
National Institute for Communicable Disease Control and Prevention
1970-2023
Beijing Center for Disease Prevention and Control
2019-2021
Baruch S. Blumberg Institute
2014-2020
Hepatitis B Foundation
2014-2020
Peking University
2015
Beijing University of Agriculture
2012-2014
Drexel University
2013-2014
IFNs are a family of cytokines that essential for the antiviral response in vertebrates. Not surprisingly, viruses have adapted to encode virulence factors cope with IFN response. Intriguingly, we show here all three types interferons, IFN-α, IFN-γ, and IFN-λ, efficiently promote infection by human coronavirus, HCoV-OC43, one major etiological agents common cold, through induction IFN-inducible transmembrane (IFITM) proteins. IFITMs typically exert their function inhibiting entry broad...
SARS-CoV-2 uses human ACE2 as a primary receptor for host cell entry. Viral entry mediated by the interaction of with spike protein largely determines range and is major constraint to interspecies transmission. We examined activity 14 orthologs found that wild-type mutant lacking furin cleavage site in S could utilize from broad animal species enter cells. These results have important implications natural hosts, transmission, models, molecular basis binding SARS-CoV-2.
ABSTRACT Chronic hepatitis B virus (HBV) infection, a serious public health problem leading to cirrhosis and hepatocellular carcinoma, is currently treated with either pegylated alpha interferon (pegIFN-α) or one of the five nucleos(t)ide analogue viral DNA polymerase inhibitors. However, neither pegIFN-α nor analogues are capable reliably curing infection. In order develop novel antiviral drugs against HBV, we established cell-based screening assay by using an immortalized mouse...
Covalently closed circular DNA (cccDNA) of hepadnaviruses exists as an episomal minichromosome in the nucleus infected hepatocyte and serves transcriptional template for viral mRNA synthesis. Elimination cccDNA is prerequisite either a therapeutic cure or immunological resolution HBV infection. Although accumulating evidence suggests that inflammatory cytokines-mediated virally hepatocytes does occur plays essential role acute infection, molecular mechanism by which cytokines eliminate...
ABSTRACT Interferon-induced transmembrane proteins (IFITMs) are restriction factors that inhibit the infectious entry of many enveloped RNA viruses. However, we demonstrated previously human IFITM2 and IFITM3 essential host facilitating coronavirus (HCoV) OC43. In a continuing effort to decipher molecular mechanism underlying IFITM differential modulation HCoV entry, investigated roles structural motifs important for protein posttranslational modifications, intracellular trafficking,...
Chronicity of hepatitis B virus (HBV) infection is due to the failure a host mount sufficient immune response clear virus. The aim this study was identify small-molecular agonists pattern recognition receptor (PRR)-mediated innate control HBV infection. To achieve goal, coupled mouse macrophage and hepatocyte culture system mimicking intrahepatic environment established used screen compounds that activate macrophages produce cytokines, which in turn suppress replication hepatocyte-derived...
Virus entry into host cells is one of the key determinants range and cell tropism subjected to control innate adaptive immune responses. In last decade, several interferon-inducible cellular proteins, including IFITMs, GILT, ADAP2, 25CH, LY6E, had been identified modulate infectious a variety viruses. Particularly, LY6E was recently as factor that facilitates human-pathogenic viruses, human immunodeficiency virus, influenza A yellow fever virus. Identification potent restriction...
The administration of COVID-19 vaccines is the primary strategy used to prevent further infections by COVID-19, especially in people living with HIV (PLWH), who are at increased risk for severe symptoms and mortality. However, vaccine hesitancy, safety, immunogenicity among PLWH have not been fully characterized. We estimated hesitancy status vaccination Chinese PLWH, explored safety impact on antiviral therapy (ART) efficacy compared an inactivated between healthy controls (HC). In total,...
Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) has become one major threat to human population health. The RNA-dependent RNA polymerase (RdRp) presents an ideal target of antivirals, whereas nucleoside analogs inhibitor is hindered by the proofreading activity coronavirus. Herein, we report that corilagin (RAI-S-37) as a non-nucleoside SARS-CoV-2 RdRp, binds directly effectively inhibits in both cell-free and cell-based assays, fully resists potently infection with low 50%...
ABSTRACT Interferon alpha (IFN-α) is an approved medication for chronic hepatitis B therapy. Besides acting as immunomodulator, IFN-α elicits a pleiotropic antiviral state in virus (HBV)-infected hepatocytes, but whether or not impedes the late steps of HBV life cycle, such secretion, remains elusive. Here we report that treatment HepAD38 cells with established replication selectively reduced virion release without altering intracellular viral secretion subviral particles and nonenveloped...
Endoplasmic reticulum (ER)-resident glucosidases I and II sequentially trim the three terminal glucose moieties on N-linked glycans attached to nascent glycoproteins. These reactions are first steps of glycan processing essential for proper folding function many Because most viral envelope glycoproteins contain glycans, inhibition ER with derivatives 1-deoxynojirimycin, i.e., iminosugars, efficiently disrupts morphogenesis a broad spectrum enveloped viruses. However, like proteins, cellular...
ABSTRACT Induction of interferon and proinflammatory cytokines is a hallmark the infection many different viruses. However, hepatitis B virus (HBV) does not elicit detectable cytokine response in infected hepatocytes. In order to investigate molecular mechanism underlying innate immune evasion, functional cyclic GMP-AMP (cGAMP) synthase (cGAS)-stimulator genes (STING) pathway was reconstituted human hepatoma cell line supporting tetracycline-inducible HBV replication. It demonstrated that...
Diverse SARS-like coronaviruses (SL-CoVs) have been identified from bats and other animal species. Like SARS-CoV, some bat SL-CoVs, such as WIV1, also use angiotensin converting enzyme 2 (ACE2) human entry receptor. However, whether these viruses can the ACE2 of species their receptor remains to be determined. We report herein that WIV1 has a broader tropism orthologs than SARS-CoV isolate Tor2. Among 9 examined, exhibited highest efficiency mediate infection pseudotyped virus. Our findings...
Spontaneous mutations introduce uncertainty into coronavirus disease 2019 (COVID-19) control procedures and vaccine development. Here, we perform a spatiotemporal analysis on intra-host single-nucleotide variants (iSNVs) in 402 clinical samples from 170 affected individuals, which reveals an increase genetic diversity over time after symptom onset individuals. Nonsynonymous are overrepresented the pool of iSNVs but underrepresented at polymorphism (SNP) level, suggesting two-step fitness...
Human coronavirus NL63 (NL63), a member of the group I coronaviruses, may cause acute respiratory diseases in young children and immunocompromised adults. Like severe syndrome (SARS-CoV), also employs human angiotensin-converting enzyme 2 (hACE2) receptor for cellular entry. To identify residues spike protein that are important hACE2 binding, this study first generated series S1-truncated variants, examined their associations with subsequently mapped minimal receptor-binding domain (RBD)...
After a short recovery period, COVID-19 reinfections could occur in convalescent patients, even those with measurable levels of neutralizing antibodies. Effective vaccinations and protective public health measures are recommended for the patients.
ABSTRACT The COVID-19 pandemic has caused an unprecedented global public health and economy crisis. origin emergence of its causal agent, SARS-CoV-2, in the human population remains mysterious, although bat pangolin were proposed to be natural reservoirs. Strikingly, comparing SARS-CoV-2-like CoVs identified bats pangolins, SARS-CoV-2 harbors a polybasic furin cleavage site spike (S) glycoprotein. uses ACE2 as receptor infect cells. Receptor recognition by S protein is major determinant host...
Interferons (IFNs) control viral infections by inducing expression of IFN-stimulated genes (ISGs) that restrict distinct steps replication. We report herein gamma-interferon-inducible lysosomal thiol reductase (GILT), a lysosome-associated ISG, restricts the infectious entry selected enveloped RNA viruses. Specifically, we demonstrated GILT was constitutively expressed in lung epithelial cells and fibroblasts its could be further induced type II interferon. While overexpression inhibited...
The yellow fever virus (YFV) recently reemerged in the large outbreaks Africa and Brazil, first imported patients into Asia have recalled concerns of YFV evolution. Here we show phylogenomics with serial clinical samples 2016 infections. Phylogenetics exhibited that strains were close to Angola 1971 only three amino acid changes presented new other lineages. Deep sequencing viral genomes discovered 101 intrahost single nucleotide variations (iSNVs) 234 polymorphisms (SNPs). Analysis iSNV...
Abstract Objectives To describe a high-sensitivity SARS-CoV-2 antigen test that is based on the fully automated light-initiated chemiluminescent immunoassay (LiCA ® ), and to validate its analytical characteristics clinical agreement detecting infection against reference molecular test. Methods Analytical performance was validated detection limits were determined using different types of nucleocapsid protein samples. 798-pair anterior nasal swab specimens collected from hospitalized patients...
Canine coronavirus-human pneumonia-2018 (CCoV-HuPn-2018) was recently isolated from a child with pneumonia. This novel human pathogen resulted cross-species transmission of canine coronavirus. It has been known that CCoV-HuPn-2018 uses aminopeptidase N (APN) canines, felines, and porcines, but not humans, as functional receptors for cell entry. The molecular mechanism entry in remains poorly understood. In this study, we demonstrated among the nine APN orthologs tested, Mexican free-tailed...