A5019382344- Immune Cell Function and Interaction
- Asthma and respiratory diseases
- HIV Research and Treatment
- HIV-related health complications and treatments
- COVID-19 Clinical Research Studies
- HIV/AIDS Research and Interventions
- Immune Response and Inflammation
- SARS-CoV-2 and COVID-19 Research
- Respiratory viral infections research
- Luminescence and Fluorescent Materials
- Immune cells in cancer
- IL-33, ST2, and ILC Pathways
- Long-Term Effects of COVID-19
- HIV/AIDS drug development and treatment
- Immunotherapy and Immune Responses
- Nanoplatforms for cancer theranostics
- T-cell and B-cell Immunology
- Pneumocystis jirovecii pneumonia detection and treatment
- RNA Interference and Gene Delivery
- Liver Disease Diagnosis and Treatment
- Molecular Sensors and Ion Detection
- Neonatal Respiratory Health Research
- Hepatocellular Carcinoma Treatment and Prognosis
- Immunodeficiency and Autoimmune Disorders
- Click Chemistry and Applications
Huazhong University of Science and Technology
2017-2025
Zhejiang University
2024
Zhejiang Wanli University
2023-2024
Harbin Medical University
2024
Beijing Ditan Hospital
2013-2023
Capital Medical University
2013-2023
Beijing Shijitan Hospital
2022-2023
Genecast (China)
2023
Children's Hospital of Fudan University
2023
National Institute for Communicable Disease Control and Prevention
2017-2022
To understand the effects of substitution patterns on photosensitizing ability boron dipyrromethene (BODIPY), two structural variations that either investigate effectiveness various iodinated derivatives to maximize "heavy atom effect" or focus effect extended conjugation at 4-pyrrolic position red-shift their activation wavelengths were investigated. Compounds with less photocytotoxic than parent unconjugated compound, while those an BODIPY core presented better photocytotoxicity compounds...
With the capability of inducing elevated expression ACE2 (angiotensin-converting enzyme 2), cellular receptor for severe acute respiratory syndrome coronavirus 2, angiotensin II blockers (ARBs) or ACE inhibitors treatment may have a controversial role in both facilitating virus infection and reducing pathogenic inflammation. We aimed to evaluate effects ARBs/ACE on disease 2019 (COVID-19) retrospective, single-center study. One hundred twenty-six patients with COVID-19 preexisting...
Most patients with severe infection influenza A virus (IAV) progress to acute respiratory distress syndrome and even multiple organ dysfunction (MODS). Neutrophil extracellular traps (NETs) can be induced by pathogens are responsible for immune tissue damage. We conducted a prospective study on the production effects of NETs in H7N9 H1N1 patients. investigated NET plasma supernatant cultured neutrophils measuring cell-free deoxyribonucleic acid (DNA) myeloperoxidase (MPO)-DNA complexes...
The objective of this work was to form water-soluble 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene (BODIPY) derivatives. Sulfonation conditions were developed for several BODIPY dyes give the monosulfonated products 1a-3a and disulfonated 1b-3b. Compounds 1 are functionalized with an aryl iodide organometallic couplings. Similarly, 2 has aromatic bromide but also two chlorine atoms that could be replaced via SNAr reactions. amine 3 is amenable couple biomolecules acylation A diazotization/azide...
A molecule that transfers energy through bonds from a donor to an acceptor was prepared with pH sensitive function (fluorescein). At values above 6.5, minimal transfer occurred, and the probe emitted green fluorescence (ca. 520 nm) when excited at (488 nm). Below 6.0 however, is efficient; hence excitation causes emission part (600 This used image conjugate of bovine serum albumin imported into endosomes or in cytosol using noncovalently bound carrier, Pep-1, 37 4 degrees C, respectively....
Treatment of acute myeloid leukemia (AML) remains challenging. Enhancement anti-tumor responses by blocking negative immune regulators is a promising strategy for novel effective therapeutics. V-domain Ig suppressor T-cell activation (VISTA) recently defined regulator mediating evasion in cancer. To investigate the effect VISTA on anti-leukemia response AML, we initiated study using clinical samples collected from AML patients. Here report that highly expressed myeloid-derived cells (MDSCs)...
Background The global outbreak of coronavirus disease 2019 (COVID-19) has turned into a worldwide public health crisis and caused more than 100,000,000 severe cases. Progressive lymphopenia, especially in T cells, was prominent clinical feature COVID-19. Activated HLA-DR + CD38 CD8 cells were enriched over prolonged period from the lymphopenia patients who died Ebola influenza infection infected with SARS-CoV-2. However, population reported to play contradictory roles SARS-CoV-2 infection....
The administration of COVID-19 vaccines is the primary strategy used to prevent further infections by COVID-19, especially in people living with HIV (PLWH), who are at increased risk for severe symptoms and mortality. However, vaccine hesitancy, safety, immunogenicity among PLWH have not been fully characterized. We estimated hesitancy status vaccination Chinese PLWH, explored safety impact on antiviral therapy (ART) efficacy compared an inactivated between healthy controls (HC). In total,...
The long and the short of it: Luminol chemiluminesces with a beautiful blue color; however, to be useful for biotechnological applications, emission must shifted much longer wavelengths. Energy-transfer cassettes like that shown in picture provide one solution.
OBJECTIVE—The implication of innate immunity in type 1 diabetes development has long been proposed. High-mobility group box (HMGB1), an evolutionarily conserved chromosomal protein, was recently recognized to be a potent inflammatory mediator when released extracellularly. We sought test the hypothesis that HMGB1 acts as immune implicated pathogenesis. RESEARCH DESIGN AND METHODS—Eight- and 12-week-old NOD mice were treated with neutralizing antibody once week until 25 weeks age monitored...
CD14(+)CD16(+) monocytes are an important cellular target for HIV-1 entry and expand in the peripheral blood of HIV-infected individuals. Because a heterogeneous population consist CD14(high)CD16(+) CD14(low)CD16(+) subsets, we evaluated effects HIV infection on distinct subsets CD16(+) monocytes.Untreated patients were recruited to investigate relationship between proportions monocyte with plasma viral loads CD4(+) T-cell counts. Patients receiving highly active antiretroviral therapy...
Recent evidence indicates that human regulatory T cells (Tregs) are composed of three distinct subpopulations: CD25(++) CD45RA(+) resting Tregs (rTregs), CD25(+++) CD45RA(-) activated (aTregs), which suppressive, and cytokine-secreting (Fr III) with pro-inflammatory capacity.To evaluate the dynamic changes in circulating pulmonary Treg subpopulations smokers patients chronic obstructive disease (COPD), to explore their potential roles COPD pathogenesis.Blood samples were obtained from 57...
Abstract With the capability of inducing elevated expression ACE2, cellular receptor for SARS-CoV-2, angiotensin II blockers or angiotensin-converting enzyme inhibitors (ARBs/ACEIs) treatment may have a controversial role in both facilitating virus infection and reducing pathogenic inflammation. We aimed to evaluate correlation ARBs/ACEIs usage with pathogenesis COVID-19 retrospective, single-center study. 126 patients preexisting hypertension at Hubei Provincial Hospital Traditional Chinese...
Spontaneous mutations introduce uncertainty into coronavirus disease 2019 (COVID-19) control procedures and vaccine development. Here, we perform a spatiotemporal analysis on intra-host single-nucleotide variants (iSNVs) in 402 clinical samples from 170 affected individuals, which reveals an increase genetic diversity over time after symptom onset individuals. Nonsynonymous are overrepresented the pool of iSNVs but underrepresented at polymorphism (SNP) level, suggesting two-step fitness...
It has been widely accepted that monocytes are one of the central mediators contributing to inflammaging. However, it remains unclear whether aged monocytes, similar T cells, have characteristics hyperactivation and increased expression co-inhibitory molecules.Peripheral blood mononuclear cells (PBMCs) were isolated from young (21-40 years old), middle-aged (41-60 older human subjects (> 60 old). Flow cytometry was used monitor changes in surface molecules monocyte subsets cytokine-producing...
Chemoselective cross-coupling reactions were demonstrated for C-S bonds in the BODIPY dyes 1 and 4, similar applied to make two-dye cassette system 11.
Photophysical data and orbital energy levels (from electrochemistry) were compared for molecules with the same BODIPY acceptor part (red) perpendicularly oriented xanthene or donor fragments (green). Transfer of energy, hence photophysical properties cassettes, including pH dependent fluorescence in xanthene-containing molecules, correlates relative energies frontier orbitals these systems. Intracellular sensing protons is often achieved via sensors that switch off completely at certain...