In cancer progression, hypoxia, or low oxygen tension, is a major regulator of tumor aggressiveness and metastasis. However, how cells adapt to the hypoxia communicate with other mesenchymal in microenvironment during development remains be elucidated. Here, we investigated involvement exosomes modulating angiogenesis enhancing metastasis esophageal squamous cell carcinoma (ESCC).Differential centrifugation, transmission electron microscopy nanoparticle tracking analysis were used isolate...

To assess the feasibility of texture analysis (TA) based on spectral attenuated inversion-recovery T2 weighted magnetic resonance imaging (SPAIR T2W-MRI) for classification hepatic hemangioma (HH), metastases (HM) and hepatocellular carcinoma (HCC). The SPAIR T2W-MRI data 162 patients with HH (n=55), HM (n=67) HCC (n=40) were retrospectively analyzed. We used two independent cohorts training (n = 112 patients) validation 50 patients). TA was performed textual parameters derived from gray...

CD14(+)CD16(+) monocytes are an important cellular target for HIV-1 entry and expand in the peripheral blood of HIV-infected individuals. Because a heterogeneous population consist CD14(high)CD16(+) CD14(low)CD16(+) subsets, we evaluated effects HIV infection on distinct subsets CD16(+) monocytes.Untreated patients were recruited to investigate relationship between proportions monocyte with plasma viral loads CD4(+) T-cell counts. Patients receiving highly active antiretroviral therapy...

Esophageal squamous cell carcinoma (ESCC) is one of the most common types cancer and leading causes cancer-related mortality worldwide, especially in Eastern Asia. Here, we downloaded microarray data lncRNA expression profiles ESCC patients from Gene Expression Omnibus (GEO) The Cancer Genome Atlas (TCGA) sets divided into training, validation test set. random survival forest (RSF) algorithm Cox regression analysis were applied to identify a seven-lncRNA signature. Then predictive ability...

Abstract As one of the most frequently diagnosed cancers, esophageal squamous cell carcinoma ( ESCC ) remains leading cause malignancy‐related death worldwide. Many studies have focused on potential role cancer cells in educating B during progression. Here, we aim to explore circulating exosomes from generation two main regulatory (Breg) subsets, including interleukin‐10 + Bregs (B10) and programmed PD )‐1 high Bregs. Firstly, observed an elevated percentage B10 peripheral blood patients...

// Yu Mao 1, * , Jian Hao Zi-Qi Jin 2 Yang-Yang Niu 3 Xue Yang 1 Dan Liu Rui Cao 4 Xiong-Zhi Wu Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Key Laboratory of Prevention Therapy, Tianjin, 300060, China University, 300070, Children's 300134, Zhong-Shan-Men Inpatient Department, These authors have contributed equally to this study share first authorship Correspondence to: Wu, email: wuxiongzhi@163.com Keywords: metastatic breast...

Abstract Serum circulating micro RNA s (c‐mi s) are serving as useful biomarkers for cancer diagnosis. Here, we describe the development of a one‐step branched rolling circle amplification ( BRCA ) method to measure serum c‐mi levels early diagnosis breast cancer. Four s, 16 (c‐miR‐16), 21 (c‐miR‐21), 155 (c‐miR‐155), and 195 (c‐miR‐195) were isolated from 49 patients (stages I‐ IV 19 healthy controls, analyzed using . The c‐miR16, c‐miR21, c‐miR155, c‐miR195 higher P < 0.0001) in stage I...

Mutations of BRAFV600E and TERT promoters are associated with thyroid cancer development. This study further investigated association these mutations clinicopathological characteristics from patients papillary carcinoma (PTC).Tumor tissues 342 PTC were obtained for DNA extraction polymerase chain reaction amplification to detect the mutation using amplification-refractory system-polymerase reaction. promoter assessed Sanger sequencing. The gene was then statistically analyzed.Two hundred...

Emerging evidence has indicated that microRNAs (miRNAs) play an important role in cervical cancer (CC). However, the of miRNA (miR)-665 remains unclear. The aim present study was to investigate potential functions miR-665 CC and identify underlying mechanisms action. Herein, we show downregulated tissues cell lines, which is negatively correlated with tumor size, distant metastasis, advanced TNM stage poor prognosis. Functionally, inhibited proliferation, migration invasion resistance...

BackgroundCircular RNAs (circRNAs) have been reported to be involved in central nervous system (CNS) diseases and a close connection with neuronal development. However, the role of circRNAs neural stem cell (NSC) differentiation treatment ischaemic stroke remains unknown.MethodsIschaemic was induced mice using transient middle cerebral artery occlusion (tMCAO). NSCs were transducted circHIPK2 siRNA (si-circHIPK2-NSCs) or vehicle control (si-circCon-NSCs) microinjected into lateral ventricle...

Autoimmune neuropathies are common peripheral nervous system (PNS) disorders. Environmental influences and dietary components known to affect the course of autoimmune diseases. Intestinal microorganisms can be dynamically regulated through diet, this study combines intestinal with diseases open up new therapeutic ideas.In Lewis rats, a model EAN was established P0 peptide, Lactobacillus were used as treatment, serum T-cell ratio, inflammatory factors, sciatic neuropathological changes,...

MicroRNAs are involved in tumor initiation and progression by regulating oncogenes suppressor genes. Here we found that miR-495 lower clinical ESCC tissues than adjacent non-tumor tissues. Moreover, the expression correlated with increased lymph node metastasis (LNM), invasion TNM stage. overexpression predicted a favorable outcome patients. targeted site 3'-UTR of Akt1, levels inversely Akt1 protein tissue samples. Overexpression suppressed cell proliferation, blocked G1/S phase transition,...

Tumors recruit bone mesenchymal stem cells (BMSCs) to localize tumor sites, which induces their conversion into cancer-associated fibroblasts (CAFs) that facilitate progression. However, this process is poorly understood on the molecular level. In study, we found 4T1 breast cancer promoted migration of BMSCs, and bFGF neutralizing antibody inhibited BMSCs induced by a tumor-conditioned medium. addition, exogenous enhanced in dose-dependent manner vitro. Furthermore, proliferation under...

Osteosarcoma is the most common primary solid malignancy of bone, and its high mortality usually correlates with early metastasis. In this study, we developed a risk score model to help predict metastasis at time diagnosis. We downloaded mined four expression profile datasets associated osteosarcoma from Gene Expression Omnibus. After data normalization, performed LASSO logistic regression analysis together 10-fold cross validation using GSE21257 dataset. A combination eight genes...

The lung-brain axis is an emerging area of study that got its basis from the gut-brain biological pathway. Using Respiratory Synctial Virus (RSV) as model respiratory viral pathogen, this aims to establish some pathways. After establishing mice model, inflammation in lung and brain were assayed using Hematoxylin-eosin staining, indirect immunofluorescence (IFA), quantitative reverse-transcription polymerase chain reaction. pathways between detected through metabolomics analysis. In lung, RSV...

Endoplasmic reticulum (ER) stress and autophagy dysfunction contribute to the establishment progression of diverse pathologies. Proteolytic activation transcription factor nSREBP1 is induced under ER stress; however, little known about how SREBP1 its nuclear active form influence unfolded protein response (UPR) in osteosarcoma cells. Our research focused on effect SREBP1/nSREBP1 upon apoptosis during molecular mechanisms involved. Here, we showed that binds promoter kinase RNA-like...

As a common cancer type in women, cervical remains one of the leading causes cancer-associated mortalities word wide. Recent evidence has demonstrated regulatory role large number long non-coding RNAs (lncRNAs) cancer. Here, we aimed to identify new biomarkers that related with recurrence through comprehensive bioinformatics analysis.Firstly, collected online lncRNA expression data patients which were divided into training, validation, and test set. Then developed nine-lncRNA signature from...

Family with sequence similarity 83 (FAM83) members play important roles in carcinogenesis and tumor progression several types. However, the mechanism by which cancer cells regulate FAM83F still remains unclear. In this study, we found that FAM84F protein messenger RNA (mRNA) levels were consistently upregulated esophageal squamous cell carcinoma (ESCC) tissues, suggests a post-transcriptional may be involved regulation of FAM83F. Since microRNAs (miRNAs) are powerful regulators gene...

The insulin-like growth factor 1 receptor (IGF1R) was recognized as a pivotal that facilitated the cellular entry of RSV. Small molecule inhibitors designed to target IGF1R exhibited potential potent antiviral agents. Through virtual screening, we conducted screening process involving small compounds derived from natural products, aiming protein against respiratory syncytial virus infection. molecular dynamics simulation analysis showed tannic acid and daptomycin interacted with IGF1R....

We introduce a reliable scheme for continuous-variable quantum key distribution (CV-QKD) by using orthogonal frequency division multiplexing (OFDM). As spectrally efficient technique, OFDM allows large number of closely spaced subcarrier signals used to carry data on several parallel streams or channels. place emphasis modulator impairments which would inevitably arise in the system and analyze how these affect OFDM-based CV-QKD system. Moreover, we also evaluate security asymptotic limit...