A5100619210- Single-cell and spatial transcriptomics
- CRISPR and Genetic Engineering
- Cancer Genomics and Diagnostics
- HIV Research and Treatment
- Immune Cell Function and Interaction
- RNA modifications and cancer
- Cytomegalovirus and herpesvirus research
- Epigenetics and DNA Methylation
- SARS-CoV-2 and COVID-19 Research
- CAR-T cell therapy research
- Virus-based gene therapy research
- Cancer Cells and Metastasis
- Cancer, Hypoxia, and Metabolism
- Genomics and Phylogenetic Studies
- Immunotherapy and Immune Responses
- Microtubule and mitosis dynamics
- T-cell and B-cell Immunology
- Advanced biosensing and bioanalysis techniques
- Innovative Microfluidic and Catalytic Techniques Innovation
- Autophagy in Disease and Therapy
- Gut microbiota and health
- Microfluidic and Bio-sensing Technologies
- AI in cancer detection
- Neuroinflammation and Neurodegeneration Mechanisms
- Ubiquitin and proteasome pathways
Tsinghua University
2008-2025
Center for Life Sciences
2017-2024
Chinese Institute for Brain Research
2020-2024
Nanchang University
2015-2024
Yangzhou University
2023-2024
Fujian Medical University
2023-2024
Nankai University
2024
Fujian Provincial Hospital
2024
Gannan Medical University
2024
Beijing University of Technology
2024
Chinese hamster ovary (CHO)-derived cell lines are the preferred host cells for production of therapeutic proteins. Here we present a draft genomic sequence CHO-K1 ancestral line. The assembly comprises 2.45 Gb sequence, with 24,383 predicted genes. We associate most assembled scaffolds 21 chromosomes isolated by microfluidics to identify chromosomal locations Furthermore, investigate genes involved in glycosylation, which affect protein quality, and viral susceptibility genes, relevant...
Alzheimer's disease and other neurodegenerative disorders of aging are characterized by clinical pathological features that relatively specific to humans. To obtain greater insight into how brain has evolved, we compared age-related gene expression changes in the cortex humans, rhesus macaques, mice on a genome-wide scale. A small subset conserved all three species, including robust age-dependent upregulation neuroprotective apolipoprotein D (APOD) downregulation synaptic cAMP signaling...
Single-cell sequencing is emerging as an important tool for studies of genomic heterogeneity. Whole genome amplification (WGA) a key step in single-cell workflows and multitude methods have been introduced. Here, we compare three state-of-the-art on both bulk samples E. coli DNA: Multiple Displacement Amplification (MDA), Annealing Looping Based Cycles (MALBAC), the PicoPLEX WGA kit (NEB-WGA). We considered effects reaction gain coverage uniformity, error rates level background...
Significance Latent viral infection is a major obstacle for effective antiviral treatment and presents continuous risk to the host. The dormant genome during latent provides few therapeutic targets other than itself drug development. This study demonstrates clearance of Epstein–Barr virus genomes in subpopulation Burkitt’s lymphoma patient-derived cells with clustered regularly interspaced short palindromic repeat/Cas9 nuclease. Viral destruction leads proliferation arrest apoptosis...
Esophageal squamous-cell carcinoma (ESCC), one of the most prevalent and lethal malignant disease, has a complex but unknown tumor ecosystem. Here, we investigate composition ESCC tumors based on 208,659 single-cell transcriptomes derived from 60 individuals. We identify 8 common expression programs epithelial cells discover 42 cell types, including 26 immune 16 nonimmune stromal subtypes in microenvironment (TME), analyse interactions between cancer other among different types TME....
supported by the National Major ScienceTthe Chinese Academy of Medical Sciences Innovation Fund for (2020-I2M-Co V19-011 and 2016-I2M-1-014);the Natural Science Foundation China (21525521);the Beijing Advanced Center Structural Biology (ICSB);the Genomics(ICG)
Highlights•We corrected the mutant CFTR gene in cystic fibrosis iPSCs•Correction restored protein expression and function iPSC-derived epithelial cells•We observed an exquisitely sensitive, homology-dependent, allele-preferred targetingSummaryRecently developed reprogramming genome editing technologies make possible derivation of patient-specific pluripotent stem cell sources—potentially useful for development new therapeutic approaches. Starting with skin fibroblasts from patients diagnosed...
Abstract The construction of 3D porous structures and metamaterials (MMs) is the most promising approaches to achieve broadband electromagnetic wave (EMW) absorption. However, it still a huge challenge multispectral ultra‐broadband EMW absorption properties compatible with low‐frequency band (L‐ S‐band) due its ultralong wavelength strong bypass capability. Herein, magnetic–dielectric synergistic gradient MMs (MDSGM) consisting oriented flaky carbonyl iron (FCI) film periodical RGO/CNT/CNF...
Inflammatory diseases are often chronic and recurrent, current treatments do not typically remove underlying disease drivers1. T cells participate in a wide range of inflammatory such as psoriasis2, Crohn's disease3, oesophagitis4 multiple sclerosis5,6, clonally expanded antigen-specific may contribute to chronicity recurrence, part by forming persistent pathogenic memory. Chronic rhinosinusitis asthma airway that present comorbidities7. affects more than 10% the general population8. Among...
Abstract The CD16+ subset of peripheral blood monocytes (Mo) is expanded dramatically during inflammatory conditions including sepsis, HIV-1 infection, and cancer. express high levels CX3CR1, which mediates arrest onto CX3CL1-expressing endothelial cells (EC) under flow conditions. In contrast, attachment CD16− Mo cytokine-activated EC independent CX3CL1. Here, we investigate the ability to produce proinflammatory cytokines upon interaction with HUVEC. We demonstrate that but not IL-6, CCL2,...