A5091509927- Esophageal Cancer Research and Treatment
- Hepatitis B Virus Studies
- Pancreatic and Hepatic Oncology Research
- Hepatitis C virus research
- Single-cell and spatial transcriptomics
- Cancer-related gene regulation
- Cancer Research and Treatments
- Cancer Genomics and Diagnostics
- Bacteriophages and microbial interactions
- Cancer, Hypoxia, and Metabolism
- Epigenetics and DNA Methylation
- Animal Virus Infections Studies
- Gastrointestinal Tumor Research and Treatment
- Viral gastroenteritis research and epidemiology
- Virus-based gene therapy research
- RNA modifications and cancer
- Genomic variations and chromosomal abnormalities
- Cancer Immunotherapy and Biomarkers
- HIV Research and Treatment
- Lattice Boltzmann Simulation Studies
- Genetic Associations and Epidemiology
- Nanofluid Flow and Heat Transfer
- Mycobacterium research and diagnosis
- DNA and Nucleic Acid Chemistry
- Advances in Oncology and Radiotherapy
Shanghai Jiao Tong University
2025
Second Affiliated Hospital of Nanjing Medical University
2024
Chinese Academy of Medical Sciences & Peking Union Medical College
2016-2022
Lanzhou Veterinary Research Institute
2021
Chinese Academy of Agricultural Sciences
2021
Southwest Minzu University
2020-2021
Academy of Medical Sciences
2020
Xi'an University of Technology
2020
National Clinical Research
2019
Dalian Medical University
2015-2018
Esophageal squamous-cell carcinoma (ESCC), one of the most prevalent and lethal malignant disease, has a complex but unknown tumor ecosystem. Here, we investigate composition ESCC tumors based on 208,659 single-cell transcriptomes derived from 60 individuals. We identify 8 common expression programs epithelial cells discover 42 cell types, including 26 immune 16 nonimmune stromal subtypes in microenvironment (TME), analyse interactions between cancer other among different types TME....
Abstract Approximately half of the world’s 500,000 new oesophageal squamous-cell carcinoma (ESCC) cases each year occur in China. Here, we show whole-genome sequencing DNA and RNA 94 Chinese individuals with ESCC. We identify six mutational signatures (E1–E6), Signature E4 is unique ESCC linked to alcohol intake genetic variants alcohol-metabolizing enzymes. discover significantly recurrent mutations 20 protein-coding genes, 4 long non-coding RNAs 10 untranslational regions. Functional...
Abstract Esophageal squamous cell carcinoma (ESCC) is prevalent in some geographical regions of the world. ESCC development presents a multistep pathogenic process from inflammation to invasive cancer; however, what critical these processes and how they evolve largely unknown, obstructing early diagnosis effective treatment. Here, we create mouse model mimicking human construct single-cell developmental atlas. We identify set key transitional signatures associated with oncogenic evolution...
Abstract This study investigates aberrant DNA methylations as potential diagnosis and prognosis markers for esophageal squamous-cell carcinoma (ESCC), which if diagnosed at advanced stages has <30% five-year survival rate. Comparing genome-wide methylation sites of 91 ESCC matched adjacent normal tissues, we identified 35,577 differentially methylated CpG (DMCs) characterized their distribution patterns. Integrating whole-genome RNA-sequencing data the same samples, found multiple...
Rationale: Whole-genome sequencing has identified many amplified genes in esophageal squamous-cell carcinoma (ESCC).This study investigated the role and clinical relevance of these ESCC.Methods: We collected ESCC non-tumor tissues from 225 individuals who underwent surgery.Clinical data were survival time was measured date diagnosis to last follow-up or death.Patient compared with immunohistochemical staining score using Kaplan-Meier methods hazard ratios calculated by Cox models.Cells gene...
Abstract Background This study aimed to investigate the interactions among three core elements of respiratory infection—pathogen, lung microbiome, and host response—and their avocation with severity outcomes Mycoplasma pneumoniae pneumonia (MPP) in children. Methods We prospectively collected bronchoalveolar lavage fluid from a cohort 41 children MPP, including general MPP (GMPP) complicated (CMPP), followed by microbiome transcriptomic analyses characterize association pathogen, response...
Adenocarcinoma at the gastroesophageal junction (ACGEJ) has dismal clinical outcomes, and there are currently few specific effective therapies because of limited knowledge on its genomic transcriptomic alterations. The present study investigates changes in ACGEJ from Chinese patients analyzes their drug vulnerabilities associations with survival time. Here we show that major chromosome instability promoted tumorigenic focal copy-number variations COSMIC Signature 17-featured single...
Infantile spasms syndrome (ISS) is an early-onset epileptic encephalopathy characterized by uncontrollable seizures, severe electroencephalogram abnormalities, as well delayed cognitive and behavioral development. Independent studies have shown that a variety of genes are involved in ISS genetic factors play critical role its pathogenesis. Here we report two de novo mutations the casein kinase 1 isoform alpha (CSNK1A1) gene which underlie epilepsy with similar clinical presentation patients....
Pancreatic ductal adenocarcinoma (PDAC) is one of the most aggressive cancers. Recent research has demonstrated that chronic pancreatitis (CP) associated with an increased risk PDAC, partly due to acinar‐to‐ductal metaplasia (ADM). Baicalein been shown exert anti‐inflammatory and anti‐tumor effects for CP or respectively. The aim our study was investigate effect baicalein, putative underlying mechanism, on inflammatory cytokines‐induced ADM rat pancreatic acinar cell line AR42J. To baicalein...
Abstract Esophageal squamous-cell carcinoma (ESCC) is one of the most lethal malignancies in world and occurs at particularly higher frequency China. While several genome-wide association studies (GWAS) germline variants whole-genome or whole-exome sequencing somatic mutations ESCC have been published, there no comprehensive database publically available for this cancer. Here, we developed Chinese Cancer Genomic Database-Esophageal Squamous Cell Carcinoma (CCGD-ESCC) database, which contains...
Genomic-scale somatic copy number alterations in healthy humans are difficult to investigate because of low occurrence rates and the structural variations’ stochastic natures. Using a Tn5-transposase-assisted single-cell whole-genome sequencing method, we sequenced over 20,000 single lymphocytes from 16 individuals. Then, with scale increased few thousand cells per individual, found that about 7.5% had large-size alterations. Trisomy 21 was most prevalent aneuploid event among all autosomal...
The capsid of the hepatitis B virus is an attractive antiviral target for developing therapies against chronic infection. Currently available core protein allosteric modulators (CpAMs) mainly affect one two major types protein-protein interactions involved in process assembly, namely, interaction between dimers. Compounds targeting monomers have not been rigorously screened due to lack screening models. We report here a cell-based assay which formation dimers indicated by split luciferase...
Esophageal squamous-cell carcinoma (ESCC), one of the most prevalent and lethal malignant disease, has a complex but unknown tumor ecosystem. Here, we decipher for first time full compositions ESCC based on analyzing 208,659 single-cell transcriptomes in derived from 60 individuals. We identify 8 essential expression programs epithelial cells discovered 43 cell types including 26 immune 17 nonimmune stromal subtypes microenvironment (TME), also explicate interactions between cancer other...
Genome-wide association studies (GWAS) have consistently identified PLCE1 as esophageal squamous cell carcinoma (ESCC) susceptibility gene; however, the functional role of variants remains to be verified. In this study, we performed fine mapping region using our previous ESCC GWAS data and 33 additional risk in locus. Here, report characterization a four-nucleotide insertion/deletion variation (rs71031566 C----/CATTT) that was associated with developing ESCC. We demonstrate for first time...
The dual-mesh hybrid numerical method is used to investigate the salt finger convection, as well heat and mass transfer, in saturated porous media. It found that at low normalized porosity of media, saline diffusion much faster than thermal diffusion. fingers grow straightly top bottom walls, which would lessen gradient stratified system. Also, it indicated perfect for dealing with small scale convection media because its great veracity, efficiency stability.
Adenocarcinoma at the gastroesophageal junction (ACGEJ) refers to a malignant tumor that occurs esophagogastric junction. Despite some progress in targeted therapies for HER2, FGFR2, EGFR, MET, Claudin 18.2 and immune checkpoints ACGEJ tumors, 5-year survival rate of patients remains poor. Thus, it is urgent explore genomic alterations neoantigen characteristics tumors identify CD8+ T-cell infiltration-associated genes find potential therapeutic targets develop risk model predict patients'...
Summary Whole-genome sequencing has identified many amplified genes in esophageal squamous-cell carcinoma (ESCC); however, their roles and the clinical relevance have yet elucidated. Here we show TP53-induced glycolysis apoptosis regulator (TIGAR) is a major player ESCC progression chemoresistance. TIGAR reprograms glucose metabolism from to glutamine pathway through AMP-activated kinase, its overexpression correlated with poor disease outcomes. Tigar knockout mice reduced growth tumor...