A5060404458- Viral Infectious Diseases and Gene Expression in Insects
- Protein purification and stability
- Advanced Proteomics Techniques and Applications
- Monoclonal and Polyclonal Antibodies Research
- Virus-based gene therapy research
- CRISPR and Genetic Engineering
- RNA and protein synthesis mechanisms
- Multiple Myeloma Research and Treatments
- Mass Spectrometry Techniques and Applications
- Alzheimer's disease research and treatments
- Immunotherapy and Immune Responses
- Metabolomics and Mass Spectrometry Studies
- Microbial Metabolic Engineering and Bioproduction
- Barrier Structure and Function Studies
- BRCA gene mutations in cancer
- Nutrition, Genetics, and Disease
- Gene expression and cancer classification
- Peptidase Inhibition and Analysis
- RNA modifications and cancer
- Microfluidic and Capillary Electrophoresis Applications
- Bacterial Genetics and Biotechnology
- Bacteriophages and microbial interactions
- Chronic Lymphocytic Leukemia Research
- CAR-T cell therapy research
- Genetics, Bioinformatics, and Biomedical Research
University of Delaware
2016-2025
National Institute for Innovation in Manufacturing Biopharmaceuticals
2019-2024
Florida Hospital Medical Group
2024
Cell Biotech (South Korea)
2023
Indiana University Indianapolis
2023
Indiana University Melvin and Bren Simon Comprehensive Cancer Center
2023
Indiana University
2021-2023
Indiana University – Purdue University Indianapolis
2022-2023
Indiana University Health
2023
Biocat
2023
Chinese hamster ovary (CHO)-derived cell lines are the preferred host cells for production of therapeutic proteins. Here we present a draft genomic sequence CHO-K1 ancestral line. The assembly comprises 2.45 Gb sequence, with 24,383 predicted genes. We associate most assembled scaffolds 21 chromosomes isolated by microfluidics to identify chromosomal locations Furthermore, investigate genes involved in glycosylation, which affect protein quality, and viral susceptibility genes, relevant...
There is no practical and reliable premortem test for Creutzfeldt–Jakob disease the related transmissible spongiform encephalopathies. Two proteins, designated 130 131, which have been detected in low concentrations cerebrospinal fluid from patients with disease, appear to be sensitive specific markers disease. Attempts identify these however, unsuccessful. We hypothesized that they may present normal brain.
An 8-plex version of an isobaric reagent for the quantitation proteins using shotgun methods is presented. The relies on amine-labeling chemistry peptides similar to 4-plex reagents. MS/MS reporter ions at 113, 114, 115, 116, 117, 118, 119, and 121 m/z are used quantify protein expression. This technology which was first applied a test mixture consisting eight resulted in accurate quantitation, has potential increase throughput analysis quantitative proteomics experiments when compared 2-...
ABSTRACT While the majority of host cell protein (HCP) impurities are effectively removed in typical downstream purification processes, a small population HCPs particularly challenging. Previous studies have identified that challenging for variety reasons. Lipoprotein lipase (LPL)—a Chinese hamster ovary (CHO) HCP functions to hydrolyze esters triglycerides—was one ten previous as being susceptible retention processing. LPL may degrade polysorbate 80 (PS‐80) and 20 (PS‐20) final product...
The process of protein synthesis in biological systems resembles a one dimensional driven lattice gas which the particles have spatial extent, covering more than site. We expand well studied totally asymmetric exclusion process, typically cover single site, to include cases with extended objects. Exact solutions can be determined for uniform closed system. analyze open system through two approaches. First, continuum limit produces modified diffusion equation particle density profiles....
The expression of the V(D)J [variable (diversity) joining elements] recombination activating genes, RAG-1 and RAG-2, has been examined during T cell development in thymus. In situ hybridization to intact thymus RNA blot analysis isolated thymic subpopulations separated on basis receptor (TCR) demonstrated that both TCR- TCR+ cortical thymocytes express RAG-2 messenger RNA's. Within population, RAG was observed immature CD4+CD8+ (double positive) cells, but not more mature CD4+CD8- or...
It is becoming increasingly appreciated that the signal transduction systems used by eukaryotic cells to achieve a variety of essential responses represent highly complex networks rather than simple linear pathways. While significant effort being made experimentally measure rate constants for individual steps in these signaling networks, many parameters required describe behavior remain unknown, or at best, estimates. With goals and caveats mind, we use methods statistical mechanics extract...
Abstract Objective To find a panel of proteins in antemortem cerebrospinal fluid (CSF) that could be used to differentiate between samples from Alzheimer's disease (AD) patients and healthy neurological control subjects. Methods For test cohort, CSF 34 AD non‐AD were separated using two‐dimensional gel electrophoresis. The resulting protein patterns analyzed the random forest multivariate statistical method. Protein spots interest identified tandem time‐of‐flight mass spectrometry. A...
ABSTRACT Downstream processing of monoclonal antibodies (mAbs) has evolved to allow the specific process for a new product be developed largely by empirical specialization platform that enables removal impurities different kinds. A more complete characterization and itself would provide insights into rational design efficient downstream processes. This work identifies characterizes host cell protein (HCP) product‐associated impurities, is, HCP species carried through processes via direct...
Hydrogel-based depots are of growing interest for release biopharmaceuticals; however, a priori selection hydrogel compositions that will retain proteins and provide desired profiles remains elusive. Toward addressing this, in this work, we have established new tool the facile assessment protein from hydrogels applied it to evaluate effectiveness mesh size estimations on predicting retention or release. Poly(ethylene glycol) (PEG)-based were formed by photoinitiated step growth...
A laser-based hydrogel degradation technique is developed that allows for local control over porosity, fabrication of 3D vascular-derived, biomimetic, hydrogel-embedded microfluidic networks, and generation two intertwining, yet independent, networks in a single construct.
A number of food allergies (eg, fish, shellfish, and nuts) are lifelong, without any disease-transforming therapies, unclear in their underlying immunology. Clinical manifestations allergy largely mediated by IgE. Although persistent IgE titers have been attributed conventionally to long-lived IgE+ plasma cells (PCs), this has not directly comprehensively tested.We sought evaluate mechanisms allergic responses allergens.We used a model peanut anaphylaxis, various knockout mice, adoptive...
Accurate and complete genome sequences are essential in biotechnology to facilitate genome-based cell engineering efforts. The current assemblies for Cricetulus griseus, the Chinese hamster, fragmented replete with gap misassemblies, consistent most short-read-based assemblies. Here, we completely resequenced C. griseus using single molecule real time sequencing merged this Illumina-based This generated a more contiguous assembly than either technology alone, reducing number of scaffolds by...
ABSTRACT During biopharmaceutical manufacturing, Chinese hamster ovary (CHO) cells produce hundreds of extracellular host cell protein (HCP) impurities, which must be removed from the therapeutic product by downstream purification operations to ensure patient safety. A subset 118 these HCPs have been reported as exceptionally difficult remove during because they co‐purify due retention characteristics on chromatographic media and/or product‐association through strongly attractive...
The purpose of this study was to investigate the prognostic value tumour-associated macrophages with a focus on micro-anatomical localisation and determine whether molecular changes epidermal growth factor receptor (EGFR) are related macrophage infiltration in resected non-small cell lung cancer (NSCLC). One hundred forty-four patients were included study. Immunohistochemistry used identify CD68+ tumour islet surrounding stroma. Epidermal mutations studied by direct sequencing. EGFR gene...
In patients with chronic lymphocytic leukemia (CLL), treatment lenalidomide induces a unique, previously uncharacterized, immune response called tumor flare reaction (TFR). The clinical significance of this remains unknown.Forty-five CLL who were treated in phase 2 trial evaluated for the features, intensity, and duration TFR. Correlation was made cellular microenvironment. Steroids prophylaxis TFR not given to Group A (n = 29) whereas B 16) received low-dose prednisone as well slow dose...
A human cell-based in vitro model that can accurately predict drug penetration into the brain as well metrics to assess these models are valuable for development of new therapeutics. Here, induced pluripotent stem cells (hPSCs) differentiated a polarized monolayer express blood-brain barrier (BBB)-specific proteins and have transendothelial electrical resistance (TEER) values greater than 2500 Ω·cm2. By assessing permeabilities several known drugs, benchmarking system evaluate permeability...
ABSTRACT Downstream purification of monoclonal antibodies (mAbs) is normally performed using a platform process that empirically tuned to optimize impurity removal for each new product. A more fundamental understanding impurities and the product itself would provide insights into rational design efficient downstream processes. This work examines chromatographic properties Chinese hamster ovary host cell protein (HCP) in non‐affinity resins commonly used polishing steps antibody purification:...
The development of low-cost processes for hydrogen production from renewable energy is tremendous importance a truly sustainable hydrogen-based system. In this article, we demonstrate the ability to utilize sunlight produce potentially and abundant biomass derivative, glucose, using WO3-based tandem photoelectrochemical cell (PEC). Thin film WO3 photoelectrodes are shown exhibit excellent photocatalytic activity towards oxidation variety complex oxygenates, with photocurrents that nearly...