A5030683908- 3D Printing in Biomedical Research
- Hydrogels: synthesis, properties, applications
- Cellular Mechanics and Interactions
- Click Chemistry and Applications
- Supramolecular Self-Assembly in Materials
- Chemical Synthesis and Analysis
- Silk-based biomaterials and applications
- Photochromic and Fluorescence Chemistry
- Electrospun Nanofibers in Biomedical Applications
- Polymer Surface Interaction Studies
- Innovative Microfluidic and Catalytic Techniques Innovation
- Cancer Cells and Metastasis
- Advanced Polymer Synthesis and Characterization
- Tissue Engineering and Regenerative Medicine
- RNA Interference and Gene Delivery
- Collagen: Extraction and Characterization
- Bone Tissue Engineering Materials
- Tendon Structure and Treatment
- Mesenchymal stem cell research
- Inhalation and Respiratory Drug Delivery
- Cell Adhesion Molecules Research
- Immune cells in cancer
- Genetics, Bioinformatics, and Biomedical Research
- Microtubule and mitosis dynamics
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
University of Delaware
2016-2025
DuPont (United States)
2016-2023
Newark Hospital
2015-2020
Georgetown University Medical Center
2019
Georgetown University
2019
Materials Science & Engineering
2014
University of Colorado Boulder
2007-2013
Howard Hughes Medical Institute
2009-2013
We report a strategy to create photodegradable poly(ethylene glycol)-based hydrogels through rapid polymerization of cytocompatible macromers for remote manipulation gel properties in situ. Postgelation control the was demonstrated introduce temporal changes, creation arbitrarily shaped features, and on-demand pendant functionality release. Channels photodegraded within hydrogel containing encapsulated cells allow cell migration. Temporal variation biochemical composition used influence...
Hydrogels are of growing interest for the delivery therapeutics to specific sites in body. For use as a vehicle, hydrophilic precursors usually laden with bioactive moieties and then directly injected site
Wound healing results from complex signaling between cells and their environment in response to injury. Fibroblasts residing within the extracellular matrix (ECM) of various connective tissues are critical for synthesis repair. Upon injury or chronic insult, these activate into wound-healing cells, called myofibroblasts, repair damaged tissue through enzyme protein secretion. However, misregulation persistence myofibroblasts can lead uncontrolled accumulation proteins, stiffening, ultimately...
A photocleavable, poly(ethylene-glycol)-based hydrogel is presented in which predictable, user-defined gradients the network's structure can be fabricated real time under cytocompatible conditions. This platform provides new opportunities to investigate how material influences cell function. Here, morphology directed spatially by degradation-induced local polymer density (see figure, scale bars: 50 μm). Detailed facts of importance specialist readers are published as "Supporting...
The relationship between polymeric hydrogel microstructure and macroscopic properties is of specific interest to the materials science polymer communities for rational design targeted applications. Specifically, research has focused on elucidating role network formation connectivity mechanical integrity degradation behavior. Here, we compared chain step polymerized, photodegradable hydrogels. Increased ductility, tensile toughness, shear strain yield were observed in polymerized hydrogels,...
Fibroblasts residing in connective tissues throughout the body are responsible for extracellular matrix (ECM) homeostasis and repair. In response to tissue damage, they activate become myofibroblasts, which have organized contractile cytoskeletons produce a myriad of proteins ECM remodeling. However, persistence myofibroblasts can lead fibrosis with excessive collagen deposition stiffening. Thus, understanding signals regulate de-activation during normal repair is critical. Substrate modulus...
Synthetic hydrogels have been widely adopted as well-defined matrices for three-dimensional (3D) cell culture, with increasing interest in systems that enable the co-culture of multiple types probing both cell–matrix and cell–cell interactions studies tissue regeneration disease. We hypothesized unique dynamic covalent chemistry self-healing could be harnessed not only encapsulation culture human cells but also subsequent construction layered 3D co-cultures. To test this, we formed using...
Hydrogel-based depots are of growing interest for release biopharmaceuticals; however, a priori selection hydrogel compositions that will retain proteins and provide desired profiles remains elusive. Toward addressing this, in this work, we have established new tool the facile assessment protein from hydrogels applied it to evaluate effectiveness mesh size estimations on predicting retention or release. Poly(ethylene glycol) (PEG)-based were formed by photoinitiated step growth...
Hydrogels that respond to multiple stimuli (<italic>i.e.</italic>, light, reducing and aqueous microenvironment) were synthesized using thiol-Michael type reaction degradation kinetics along with release of nanobeads was characterized.
Thiol–ene photoclick hydrogels with tunable biomechanical and biochemical properties for biological applications, including controlled cell culture, regenerative medicine, drug delivery.
Photolabile moieties have been utilized in applications ranging from peptide synthesis and controlled protein activation to tunable dynamic materials. The photochromic properties of nitrobenzyl (NB) based linkers are readily tuned respond cytocompatible light doses widely cell culture other biological applications. While utilized, little is known about how the microenvironment, particularly confined aqueous environments (e.g., hydrogels), affects both mode rate cleavage NB moieties, leading...
Cell adhesion and detachment to from the extracellular matrix (ECM) are critical regulators of cell function fate due exchange mechanical signals between its microenvironment. To study this mechanobiology, researchers have developed several innovative methods investigate in vitro; however, most these culture platforms unnaturally stiff or static. better capture soft, dynamic nature ECM, we present a PEG-based hydrogel which context geometry space can be precisely controlled situvia...
Culturing mammalian neurons in three-dimensional (3D) microenvironments that more closely recapitulate critical biochemical and biophysical aspects of the developing or adult central nervous system (CNS) milieu remains a significant challenge neurobiological studies regenerative medicine. Here, we aimed to exploit recent advances poly(ethylene glycol) (PEG) hydrogel chemistries define synthetic niche capable supporting culture axonal outgrowth both aggregated dissociated mouse embryonic stem...
Crosslinks that can undergo click bond cleavage and ester hydrolysis were incorporated to design glutathione-sensitive, dually degradable hydrogels for degradation-mediated, controlled release of cargo molecules.
Mesenchymal stem cells (MSCs) are promising for the regeneration of tendon and ligament tissues. Toward realizing this potential, microenvironment conditions needed promoting robust lineage-specific differentiation into tenocytes/ligament fibroblasts. Here, we utilized a statistical design experiments approach to examine combinations matrix modulus, composition, soluble factors in human MSC tenogenic/ligamentogenic differentiation. Specifically, well-defined poly(ethylene glycol)-based...
Synthetic multifunctional assembling peptides were designed to mimic the structure of collagen and allow independent control hydrogel mechanical biochemical properties through covalent crosslinking, enabling long-term <italic>in vitro</italic> 3D cell culture.
Bacterial cells are surrounded by a dynamic cell wall which in part is made up of mesh-like peptidoglycan (PG) layer that provides the with structural integrity and resilience. In Gram-positive bacteria, this thick robust, whereas Gram-negative it thinner flexible as supported an additional outer membrane. PG undergoes continuous turnover, degradation products being recycled to maintain homeostasis. Some species can bypass de novo biosynthesis, relying instead on recycling sustain growth...
Journal Article Responsive culture platform to examine the influence of microenvironmental geometry on cell function in 3D Get access April M. Kloxin, Kloxin Chemical and Biological Engineering, University Colorado, Boulder, CO, USA. Tel: +1 (303)-735-5336 Search for other works by this author on: Oxford Academic Google Scholar Katherine J. R. Lewis, Lewis Cole A. DeForest, DeForest Gregory Seedorf, Seedorf Pediatric Heart Lung Center Laboratory, Denver, USA Mark W. Tibbitt, Tibbitt Vivek...