A5014957319- Erythrocyte Function and Pathophysiology
- Fungal and yeast genetics research
- Blood properties and coagulation
- Fungal Biology and Applications
- Cellular Mechanics and Interactions
- RNA and protein synthesis mechanisms
- RNA Research and Splicing
- Click Chemistry and Applications
- Cell Adhesion Molecules Research
- Microtubule and mitosis dynamics
- Biotin and Related Studies
- Cardiomyopathy and Myosin Studies
- Biofuel production and bioconversion
- Vibrio bacteria research studies
- Immunodeficiency and Autoimmune Disorders
- Legionella and Acanthamoeba research
- Receptor Mechanisms and Signaling
- Virus-based gene therapy research
- Monoclonal and Polyclonal Antibodies Research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Bacteriophages and microbial interactions
- Genetics, Aging, and Longevity in Model Organisms
- Biochemical Analysis and Sensing Techniques
- Protein Tyrosine Phosphatases
- Blood groups and transfusion
University of Delaware
2020-2025
Biotechnology Institute
2024
University of California, Riverside
2013-2020
University of California, San Diego
2018
-Acetyl muramic acid (NAM) probes containing alkyne or azide groups are commonly used to investigate aspects of cell wall synthesis because their small size and ability incorporate into bacterial peptidoglycan (PG). However, copper-catalyzed alkyne-azide cycloaddition (CuAAC) reactions not compatible with live cells, strain-promoted (SPAAC) reaction rates modest and, therefore, as desirable for tracking the temporal alterations growth, remodeling, division. Alternatively, tetrazine-
Bacterial cells are surrounded by a dynamic cell wall which in part is made up of mesh-like peptidoglycan (PG) layer that provides the with structural integrity and resilience. In Gram-positive bacteria, this thick robust, whereas Gram-negative it thinner flexible as supported an additional outer membrane. PG undergoes continuous turnover, degradation products being recycled to maintain homeostasis. Some species can bypass de novo biosynthesis, relying instead on recycling sustain growth...
Abstract Protein phosphatases are integral components of the cellular signaling machinery in eukaryotes, regulating diverse aspects growth and development. The genome filamentous fungus model organism Neurospora crassa encodes catalytic subunits for 30 protein phosphatase genes. In this study, we have characterized 24 viable N. subunit knockout mutants phenotypes during growth, asexual development, sexual We found that 91% had defects at least one these traits, whereas 29% possessed all...
Previous genetic and biochemical studies from Saccharomyces cerevisiae have identified a critical ribosome-associated quality control complex (RQC) that facilitates resolution of stalled ribosomal complexes. While components the mammalian RQC been examined in vitro, systematic characterization protein interactions cells has yet to be described. Here we utilize both proximity-labeling proteomic approaches, BioID APEX, traditional affinity-based strategies identify interacting proteins members...
The filamentous fungus Neurospora crassa decomposes lignocellulosic biomass to generate soluble sugars as carbon sources. In this study, we investigated a role for heterotrimeric G-protein signaling in cellulose degradation. Loss of the Gα subunit genes gna-1 and gna-3, Gβ gnb-1 cpc-2, Gγ gene gng-1, or downstream effector adenylyl cyclase (cr-1) resulted loss detectable cellulase activity. This defect was also observed strains expressing constitutively active version gna-3 (gna-3Q208L ). We...
Receptor for Activated C Kinase-1 (RACK1) is a multifunctional eukaryotic scaffolding protein with seven WD repeat structure. Among their many cellular roles, RACK1 homologs have been shown to serve as alternative Gβ subunits during heterotrimeric G signaling in systems. We investigated genetic interactions between the homolog cpc-2, previously characterized subunit gnb-1 and other components multicellular filamentous fungus Neurospora crassa. Results from cell fractionation studies...
Abstract Mammalian red blood cells are generated via a terminal erythroid differentiation pathway culminating in cell polarization and enucleation. Actin filament polymerization is critical for enucleation, but the molecular regulatory mechanisms remain poorly understood. We utilized publicly available RNA-seq proteomics datasets to mine actin-binding proteins actin- nucleation factors differentially expressed during human discovered that focal adhesion protein—Tensin-1—dramatically...
Erythroid differentiation (ED) is a complex cellular process entailing morphologically distinct maturation stages of erythroblasts during terminal differentiation. Studies actin filament (F-actin) assembly and organization ED have revealed essential roles for the F-actin pointed-end capping proteins, tropomodulins (Tmod1 Tmod3). Tmods bind tropomyosins (Tpms), which enhance Tmod stabilization. can also nucleate assembly, independent Tpms. Tmod1 present in red blood cell (RBC) membrane...
Abstract Erythroid differentiation (ED) is a complex cellular process entailing morphologically distinct maturation stages of erythroblasts during terminal differentiation. Studies actin filament assembly and organization ED have revealed essential roles for the pointed-end capping proteins, tropomodulins (Tmod1 Tmod3). Additionally, tropomyosin (Tpm) binding to Tmods key feature promoting Tmod-mediated capping. Global deletion Tmod3 leads embryonic lethality in mice with impaired ED. To...