A5089483150- Protein purification and stability
- Enzyme Structure and Function
- Protein Structure and Dynamics
- Microfluidic and Capillary Electrophoresis Applications
- Analytical Chemistry and Chromatography
- Monoclonal and Polyclonal Antibodies Research
- Viral Infectious Diseases and Gene Expression in Insects
- Crystallization and Solubility Studies
- Electrostatics and Colloid Interactions
- Polymer Surface Interaction Studies
- Material Dynamics and Properties
- Proteins in Food Systems
- Spectroscopy and Quantum Chemical Studies
- Microfluidic and Bio-sensing Technologies
- Force Microscopy Techniques and Applications
- Chromatography in Natural Products
- Nanopore and Nanochannel Transport Studies
- Lipid Membrane Structure and Behavior
- Mass Spectrometry Techniques and Applications
- Protein Interaction Studies and Fluorescence Analysis
- Gold and Silver Nanoparticles Synthesis and Applications
- Heart Rate Variability and Autonomic Control
- Fluid Dynamics and Turbulent Flows
- Heat and Mass Transfer in Porous Media
- Photonic Crystals and Applications
University of Delaware
2016-2025
Bioprocess Control (Sweden)
2023
Cell Biotech (South Korea)
2020
Bioengineering Center
2015
National Center for Genetic Engineering and Biotechnology
2015
John Wiley & Sons (United States)
2015
GTx (United States)
2007
Biotechnology Institute
2006
Johns Hopkins University
2005
United States Army
2002
Microstructured particles were synthesized by growing colloidal crystals in aqueous droplets suspended on fluorinated oil. The template highly ordered and smooth particle assemblies, which diffract light have remarkable structural stability. method allows control of size shape from spheres through ellipsoids to toroids varying the droplet composition. Cocrystallization mixtures yields anisotropic organic inorganic materials that can, for example, be oriented turned over magnetic fields....
ABSTRACT While the majority of host cell protein (HCP) impurities are effectively removed in typical downstream purification processes, a small population HCPs particularly challenging. Previous studies have identified that challenging for variety reasons. Lipoprotein lipase (LPL)—a Chinese hamster ovary (CHO) HCP functions to hydrolyze esters triglycerides—was one ten previous as being susceptible retention processing. LPL may degrade polysorbate 80 (PS‐80) and 20 (PS‐20) final product...
ADVERTISEMENT RETURN TO ISSUEPREVCommunicationNEXTAssembly of Gold Nanostructured Films Templated by Colloidal Crystals and Use in Surface-Enhanced Raman SpectroscopyPeter M. Tessier, Orlin D. Velev, Anand T. Kalambur, John F. Rabolt, Abraham Lenhoff, Eric W. KalerView Author Information Department Chemical Engineering Materials Science University Delaware, Newark, Delaware 19716 Cite this: J. Am. Chem. Soc. 2000, 122, 39, 9554–9555Publication Date (Web):September 16, 2000Publication History...
A novel scheme for using modified colloidal crystals as templates silica polymerization is reported. 3D close-packed of submicrometer latex spheres are assembled on a membrane surface by filtration. To induce polymerization, the particles adsorption surfactant hexadecyltrimethylammonium bromide. The then infused with solution, which polymerizes in cavities. In final stage, removed calcination, leaving behind porous very low density. Scanning electron microscopy images demonstrate that...
Abstract A boundary element method is developed to compute the electrostatic potential inside and around molecules in an electrolyte solution. set of integral equations are derived based on formulations Poisson equation linearized Poisson‐Boltzmann equation. The then solved numerically after discretizing molecular surface into a number flat triangular elements. applied spherical molecule for which analytical solutions available. Use made both constant linearly varying unknowns over elements,...
The second osmotic virial coefficients of seven proteins-ovalbumin, ribonuclease A, bovine serum albumin, alpha-lactalbumin, myoglobin, cytochrome c, and catalase-were measured in salt solutions. Comparison the interaction trends terms dimensionless coefficient b(2) shows that, at low concentrations, protein-protein interactions can be either attractive or repulsive, possibly due to anisotropy protein charge distribution. At high behavior depends on salt: In sodium chloride, generally show...
ABSTRACT Downstream processing of monoclonal antibodies (mAbs) has evolved to allow the specific process for a new product be developed largely by empirical specialization platform that enables removal impurities different kinds. A more complete characterization and itself would provide insights into rational design efficient downstream processes. This work identifies characterizes host cell protein (HCP) product‐associated impurities, is, HCP species carried through processes via direct...
ABSTRACT During biopharmaceutical manufacturing, Chinese hamster ovary (CHO) cells produce hundreds of extracellular host cell protein (HCP) impurities, which must be removed from the therapeutic product by downstream purification operations to ensure patient safety. A subset 118 these HCPs have been reported as exceptionally difficult remove during because they co‐purify due retention characteristics on chromatographic media and/or product‐association through strongly attractive...
Abstract While high‐throughput (HT) experimentation and mechanistic modeling have long been employed in chromatographic process development, it remains unclear how these techniques should be used concert within development workflows. In this work, a workflow based on HT experiments was constructed. The integration of approaches offers improved efficiency speed. This silico (HT‐IS) to develop Capto MMC polishing step for mAb aggregate removal. High‐throughput batch isotherm data first...
Two new methods of preparing thin porous metallic films, based on controlled co-crystallization latex microspheres and gold nanoparticles, have been developed by these authors. The materials show interesting optical properties excellent surface-enhanced Raman spectroscopy (SERS) activity. Figure shows a square pore array obtained at specific film thickness.
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTElectrostatic and van der Waals contributions to protein adsorption: computation of equilibrium constantsCharles M. Roth Abraham LenhoffCite this: Langmuir 1993, 9, 4, 962–972Publication Date (Print):April 1, 1993Publication History Published online1 May 2002Published inissue 1 April 1993https://doi.org/10.1021/la00028a015RIGHTS & PERMISSIONSArticle Views1051Altmetric-Citations139LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum...