A5054454622- Protein purification and stability
- Analytical Chemistry and Chromatography
- Monoclonal and Polyclonal Antibodies Research
- Viral Infectious Diseases and Gene Expression in Insects
- Advanced Chemical Sensor Technologies
- Metabolomics and Mass Spectrometry Studies
- Microfluidic and Capillary Electrophoresis Applications
- Nanofabrication and Lithography Techniques
- Granular flow and fluidized beds
- Chemical Synthesis and Analysis
- Chromatography in Natural Products
- Innovative Microfluidic and Catalytic Techniques Innovation
- Bacteriophages and microbial interactions
Rensselaer Polytechnic Institute
2020-2025
Abstract While high‐throughput (HT) experimentation and mechanistic modeling have long been employed in chromatographic process development, it remains unclear how these techniques should be used concert within development workflows. In this work, a workflow based on HT experiments was constructed. The integration of approaches offers improved efficiency speed. This silico (HT‐IS) to develop Capto MMC polishing step for mAb aggregate removal. High‐throughput batch isotherm data first...
Abstract Platforms have long been implemented for downstream process development of monoclonal antibodies (mAbs) to streamline and reduce timelines. These platforms are also increasingly being used other complex biologics modalities. While has traditionally conducted at the lab bench scale in a sequential manner, automated miniaturized parallelized approaches like RoboColumns resin plates chromatographic screening. Additionally, mechanistic modeling separations seen increased use...
While column modeling has been long studied for single-mode chromatographic systems, its application multimodal systems limited. Although a range of isotherm formalisms have applied to chromatography, no direct comparisons their efficacies performed. This results in lack clarity with respect which models should be employed and is clear obstacle chromatography. In this paper, set ranging complexities, accounting electrostatic interactions, hydrophobic thermodynamic activities, were examined...
While column modeling has been long studied for single-mode chromatographic systems, its application multimodal systems limited. Although a range of isotherm formalisms have applied to chromatography, no direct comparisons their efficacies performed. This results in lack clarity with respect which models should be employed and is clear obstacle chromatography. In this paper, set ranging complexities, accounting electrostatic interactions, hydrophobic thermodynamic activities, were examined...
The polishing step in the downstream processing of therapeutic antibodies is tasked with clearing residual contaminants from Protein A eluates. Among various classes impurities, antibody fragments are especially challenging to remove due broad biomolecular diversity generated by a multitude fragmentation patterns. current approach fragment removal relies on ion exchange or mixed-mode adsorbents operated bind-&-gradient-elution mode. However, that bear strong similarity intact product whose...
Column modeling, although having existed for decades, has seen limited application towards multimodal chromatographic systems. While a handful of isotherm formalisms have been developed and applied to chromatography, there not yet comprehensive investigation their relative merits. Without this kind comparison, it is still unclear which formalism appropriate use model development. This presents hurdle in modeling where prediction the already complex behavior further convoluted by lack...