A5078658364- Monoclonal and Polyclonal Antibodies Research
- Protein purification and stability
- Viral Infectious Diseases and Gene Expression in Insects
- Virus-based gene therapy research
- RNA Interference and Gene Delivery
- Analytical Chemistry and Chromatography
- Chemical Synthesis and Analysis
- Viral Infections and Immunology Research
- Protein Interaction Studies and Fluorescence Analysis
- Blood groups and transfusion
- CAR-T cell therapy research
- Microfluidic and Capillary Electrophoresis Applications
- Glycosylation and Glycoproteins Research
- Platelet Disorders and Treatments
- Silk-based biomaterials and applications
- Bacteriophages and microbial interactions
- RNA and protein synthesis mechanisms
- CRISPR and Genetic Engineering
- Oxidative Organic Chemistry Reactions
- Semiconductor materials and devices
- Inhalation and Respiratory Drug Delivery
- Click Chemistry and Applications
- Advanced biosensing and bioanalysis techniques
- Nanofabrication and Lithography Techniques
- Hemophilia Treatment and Research
North Carolina State University
2020-2025
North Central State College
2023-2024
Zhejiang University
2015-2020
The growth of advanced analytics in manufacturing monoclonal antibodies (mAbs) has highlighted the challenges associated with clearance host cell proteins (HCPs). Of special concern is removal "persistent" HCPs, including immunogenic and mAb-degrading proteins, that co-elute from Protein A resin can escape polishing steps. Responding to this challenge, we introduced an ensemble peptide ligands target HCPs Chinese hamster ovary (CHO) culture fluids enable mAb purification via flow-through...
Abstract Adeno‐associated viruses (AAVs) are the vector of choice for delivering gene therapies that can cure inherited and acquired diseases. Clinical research on various AAV serotypes significantly increased in recent years alongside regulatory approvals AAV‐based therapies. The current purification platform hinges capture step, which several affinity resins commercially available. These adsorbents rely protein ligands—typically camelid antibodies—that provide high binding capacity...
Adeno-associated viruses (AAVs) have acquired a central role in modern medicine as delivery agents for gene therapies targeting rare diseases. While new AAVs with improved tissue targeting, potency, and safety are being introduced, their biomanufacturing technology is lagging. In particular, the AAV purification pipeline hinges on protein ligands affinity-based capture step. featuring excellent binding capacity selectivity, these require strong acid (pH <3) elution conditions, which can...
Abstract Exosomes are gaining prominence as vectors for drug delivery, vaccination, and regenerative medicine. Owing to their surface biochemistry, which reflects the parent cell membrane, these nanoscale biologics feature low immunogenicity, tunable tissue tropism, ability carry a variety of payloads across biological barriers. The heterogeneity exosomes' size composition, however, makes purification challenging. Traditional techniques, like ultracentrifugation filtration, afford product...
Adenoviral vectors (AdVs) are gaining prominence in cancer therapy and vaccine development, posing the need for a modern AdV manufacturing platform. Current purification by ion-exchange chromatography indeed struggles to achieve product's yield purity of processes that employ affinity technologies. Addressing these challenges, this study presents first affinity-based process delivers high product clearance host cell proteins DNA (HCPs hcDNA) two steps. The capture utilizes resins...
The use of benign stimuli to control the binding and release labile biologics for their isolation from complex feedstocks is a key goal modern biopharmaceutical technology. This study introduces cyclic azobenzene-peptide (CAP) hybrid ligands rapid discrete photo-responsive capture blood coagulation Factor VIII (FVIII). A predictive method - based on amino acid sequence molecular architecture CAPs was developed correlate conformation cis/trans CAP photo-isomers FVIII release. combined in...
Exosomes are gaining prominence as vectors for drug delivery, vaccination, and regenerative medicine. Owing to their surface biochemistry, which reflects the parent cell membrane, these nanoscale biologics feature low immunogenicity, tunable tissue tropism, ability carry a variety of payloads across biological barriers. The heterogeneity exosomes' size composition, however, makes purification challenging. Traditional techniques, like ultracentrifugation filtration, afford product yield...
Due to the shortage of blood donation and safe issues blood-derived pathogens, recombinant human serum albumin (rHSA) expressed by Pichia pastoris is a potential substitute for plasma-derived HSA (pHSA). In this work, four commercialized mixed-mode resins (Capto adhere, Capto MMC, MX-Trp-650M, Nuvia cPrime) two ion-exchange (Q Sepharose FF SP FF) were used rHSA separation from cell culture broth, loading elution conditions optimized via high-throughput process using microtiter filter plates....
Abstract Human serum albumin is widely used in clinical practice, and the development of new ligands with high affinity beneficial to improve its separation efficiency. The Site II human an active binding site various molecules such as l ‐tryptophan, which was studied molecular simulation obtain insights for design ligands. results showed that carboxyl indolyl groups ‐tryptophan were critical on II. Seven containing designed, indole‐3‐pentanoic acid best ligand. A ligand combined...
Adeno-associated viruses (AAVs) have acquired a central role in modern medicine as delivery agents for gene therapies targeting rare diseases. While new AAVs with improved tissue targeting, potency, and safety are being introduced, their biomanufacturing technology is lagging. The AAV purification pipeline, particular, hinges on protein ligands the affinity-based capture step: while featuring excellent binding capacity selectivity, these require strong acid (pH <3) elution conditions,...