A5025757696- Monoclonal and Polyclonal Antibodies Research
- Viral Infectious Diseases and Gene Expression in Insects
- Protein purification and stability
- Microfluidic and Bio-sensing Technologies
- Innovative Microfluidic and Catalytic Techniques Innovation
- Genital Health and Disease
- DNA and Biological Computing
- Chemical Synthesis and Analysis
- Advanced biosensing and bioanalysis techniques
- Herpesvirus Infections and Treatments
- Orthopedic Infections and Treatments
- Microfluidic and Capillary Electrophoresis Applications
- RNA Interference and Gene Delivery
- Virus-based gene therapy research
- Transgenic Plants and Applications
- Thyroid Disorders and Treatments
- 3D Printing in Biomedical Research
- Viral gastroenteritis research and epidemiology
- Periodontal Regeneration and Treatments
- CRISPR and Genetic Engineering
North Carolina State University
2019-2023
Biocat
2023
BridgeBio (United States)
2020-2023
North Central State College
2019
Infectious arthritis is difficult to treat in both human and veterinary clinical practice. Recent literature reports Staphylococcus aureus as well other gram-positive gram-negative isolates forming free-floating biofilms equine synovial fluid that are tolerant traditional antimicrobial therapy. Using an vitro model, we investigated the ability of platelet-rich plasma (PRP) formulations combat biofilm aggregates. Synovial was infected, aggregates allowed form over a 2-hour period. PRP...
Abstract Host‐cell proteins (HCPs) are the foremost class of process‐related impurities to be controlled and removed in downstream processing steps monoclonal antibody (mAb) manufacturing. However, some HCPs may evade clearance multiple purification reach final drug product, potentially threatening stability patient safety. This study extends prior work on HCP characterization persistence mAb process streams by using mass spectrometry (MS)‐based methods track through for seven mAbs that were...
The growing integration of quality-by-design (QbD) concepts in biomanufacturing calls for a detailed and quantitative knowledge the profile impurities their impact on product safety efficacy. Particularly valuable is determination residual level host cell proteins (HCPs) secreted, together with interest, by recombinant cells utilized production. Though often referred to as single impurity, HCPs comprise variety species diverse abundance, size, function, composition. clearance these complex...
While antibodies remain established therapeutic and diagnostic tools, other protein scaffolds are emerging as effective safer alternatives. Affibodies in particular a new class of small proteins marketed bio-analytic reagents. They feature tailorable binding affinity, low immunogenicity, high tissue permeation, expression titer bacterial hosts. This work presents the development affibody-binding peptides to be utilized ligands for their purification from lysates. Affibody-binding candidates...
Adeno-associated virus-based gene therapies have demonstrated substantial therapeutic benefit for the treatment of genetic disorders. In manufacturing processes, viral capsids are produced with and without encapsidated interest. Capsids devoid interest, or "empty" capsids, represent a product-related impurity. As result, robust scalable method to enrich full is crucial provide patients as much potentially active product possible. Anion exchange chromatography has emerged highly utilized...
The ability to rapidly and accurately evaluate bioactive compounds immobilized on porous particles is crucial in the discovery of drugs, diagnostic reagents, ligands, catalysts. Existing options for solid phase screening compounds, while highly effective well established, can be cost-prohibitive proof-of-concept early stage work, limiting its applicability flexibility new research areas. Here, we present a low-cost microfluidics-based platform enabling automated small beads from solid-phase...
The clearance of host cell proteins (HCPs) is crucial importance in biomanufacturing, given their diversity composition, structure, abundance, and occasional structural homology with the product. current approach to HCP manufacturing monoclonal antibodies (mAbs) relies on product capture Protein A followed by removal residual HCPs flow-through mode using ion exchange or mixed-mode chromatography. Recent studies have highlighted presence "problematic HCP" species, which are either difficult...
The rapid expansion of CRISPR in biotechnology, medicine, and bioprocessing poses an urgent need for advanced manufacturing Cas nucleases. lack Cas-targeting ligands, however, prevents the development platform processes purifying this class molecules. This work represents first effort at developing short synthetic Cas9-binding peptides demonstrates their applicability as affinity ligands purification a nuclease. Candidate Cas9-targeting were initially identified by screening solid-phase...
Screening solid-phase combinatorial libraries of bioactive compounds against fluorescently labeled target biomolecules is an established technology in ligand and drug discovery. Rarely, however, do screening methods include comprehensive strategies—beyond mere library blocking competitive screening—to ensure binding selectivity selected leads. This work presents a method for multiplexed peptide using ClonePix 2 Colony Picker that integrates (i) orthogonal fluorescent labeling positive...